Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals &lt;1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Tectonic evolution of fault bounded continental blocks: Comparison of paleomagnetic and GPS data in the Corinth and Megara basins (Greece)

We report on new paleomagnetic and anisotropy of magnetic susceptibility (AMS) data from Plio-Pleistocene sedimentary units from Corinth and Megara basins (Peloponnesus, Greece). Paleomagnetic results show that Megara basin has undergone vertical axis CW rotation since the Pliocene, while Corinth has rotated CCW during the same period of time. These results indicate that the overall deformation in central Greece has been achieved by complex interactions of mostly rigid, rotating, fault bounded crustal blocks. The comparison of paleomagnetic results and existing GPS data shows that the boundaries of the rigid blocks in central Greece have changed over time, with faulting migrating into the hanging walls, sometimes changing in orientation. The Megara basin belonged to the Beotia-Locris block in the past but has now been incorporated into the Peloponnesus block, possibly because the faulting in the Gulf of Corinth has propagated both north and east. Paleomagnetic and GPS data from Megara and Corinth basins have significant implications for the deformation style of the continental lithosphere. In areas of distributed deformation the continental lithosphere behaves instantaneously like a small number of rigid blocks with well-defined boundaries. This means that these boundaries could be detected with only few years of observations with GPS. However, on a larger time interval the block boundaries change with time as the active fault moves. Paleomagnetic studies distinguishing differential rotational domains provide a useful tool to map how block boundaries change with time

An archaeometric and archaeological approach to Hellenistic–Early Roman ceramic workshops in Greece: Contribution to dating

The present article comprises a multidisciplinary archaeometric approach for the study of Hellenistic and Early Roman kilns in Greece. A collection of previously published and new archaeomagnetic data are combined with new results from mineralogical analytical experiments. The sampled material came from four areas, covering different geological contexts: Katerini, Olympiada, and Polymylos in mainland Greece, and the island of Paros. Extensive rock-magnetic experiments, including identification of the dominant ferromagnetic minerals present, their domain state, and mineralogical alterations during laboratory treatments, have been carried out in order to examine the magnetic properties of the studied materials and prove their suitability for reliable archaeomagnetic determinations. Magnetic cleaning provided well-defined archaeomagnetic directions, and archaeointensity measurements were carried out using both the Thellier-Thellier and Triaxe protocols. Information from both magnetic and mineralogical properties referring to firing conditions is further discussed along with archaeological information. Finally, a new dating of the four sites together with other structures of similar age was carried out using the Pavón-Carrasco model.Center for Mediterranean Archaeology and the Environment (CMATE) Special Issue, Joint publication of Radiocarbon and Tree-Ring Research, also cited as Kondopoulou, D., Zananiri, I., Rathossi, C., Marco, E., Spatharas, V., & Hasaki, E. (2014). An Archaeometric and Archaeological Approach to Hellenistic-Early Roman Ceramic Workshops in Greece: Contribution to Dating. Radiocarbon, 56(4), S27-S38.This item is part of the Tree-Ring Research (formerly Tree-Ring Bulletin) archive. For more information about this peer-reviewed scholarly journal, please email the Editor of Tree-Ring Research at [email protected]

New archaeointensity results from archaeological sites and variation of the geomagnetic field intensity for the last 7 millennia in Greece

International audienceIn this study six new intensity determinations are presented, obtained from five well dated archaeological sites, located in northern Greece and in Paros, Cyclades Islands. The fired structures consisted of ceramic and pottery kilns belonging to the Hellenistic, Roman and Byzantine periods. Between 8 and 21 samples of highly fired baked clays, tiles and bricks were taken, homogeneously distributed over the structures. The samples were analysed using the classical Thellier method, providing the past intensities and directions of the geomagnetic field recorded at each site. The intensity values have been corrected for anisotropy of thermal remanent magnetisation and cooling rate effects. Differences in the mean archaeointensities per site ranging from 1% to 11%, before and after TRM anisotropy and cooling rate corrections, were obtained. The new results indicate a decrease of 20% of the geomagnetic field strength in Greece, during the last four centuries BC. In order to compare our results with previously published data, a catalogue of archaeo- and palaeointensity results for the Aegean area has been established, covering the last 7 millennia. It consists of 336 data from Greece, western Turkey and Former Yugoslavia, collected from various authors. Weighting factors have been applied to these data, that then have been treated with a hierarchical Bayesian modelling, and a geomagnetic field intensity variation curve for Greece was constructed. A good agreement is observed when comparing the curve for Greece with the Bulgarian secular variation curve (SVC) for intensity. Satisfactory coincidence is also found with the archaeointensity data from Mesopotamia. Despite the presence of some time gaps, a more precise secular variation intensity curve has been constructed for Greece which, combined with a forthcoming directional SVC, will help for dating purposes

Tectonic evolution of fault-bounded continental blocks:

We report on new paleomagnetic and anisotropy of magnetic susceptibility (AMS) data from Plio-Pleistocene sedimentary units from Corinth and Megara basins (Peloponnesus, Greece). Paleomagnetic results show that Megara basin has undergone vertical axis CW rotation since the Pliocene, while Corinth has rotated CCW during the same period of time. These results indicate that the overall deformation in central Greece has been achieved by complex interactions of mostly rigid, rotating, fault bounded crustal blocks. The comparison of paleomagnetic results and existing GPS data shows that the boundaries of the rigid blocks in central Greece have changed over time, with faulting migrating into the hanging walls, sometimes changing in orientation. The Megara basin belonged to the Beotia-Locris block in the past but has now been incorporated into the Peloponnesus block, possibly because the faulting in the Gulf of Corinth has propagated both north and east. Paleomagnetic and GPS data from Megara and Corinth basins have significant implications for the deformation style of the continental lithosphere. In areas of distributed deformation the continental lithosphere behaves instantaneously like a small number of rigid blocks with well-defined boundaries. This means that these boundaries could be detected with only few years of observations with GPS. However, on a larger time interval the block boundaries change with time as the active fault moves. Paleomagnetic studies distinguishing differential rotational domains provide a useful tool to map how block boundaries change with time