IR quantification of fenofibric acid in bulk and oral dosage form

Simple and sensitive infrared spectrophotometric method has been developed for the estimation of fenofibric acid in tablet dosage form.Beer?s concentration range was found to lie between 5 to 15 mg. The correlation coefficient for the method was found to be 0.9946 and the developed method was analyzed for specificity, linearity of response, precision and accuracy; thus the proposed method could be adopted for routine analysis of bulk drug and its formulation

Evaluation of In Vitro Anti-inflammatory Activity of Azomethines of Aryl Oxazoles

Abstract: Ability to inhibit erythrocyte hemolysis is often used as a characteristic of the membrane stabilising action of chemical compounds. Azomethines of aryl oxazoles were evaluated for anti-inflammatory by in vitro hemolytic membrane stabilising study. The effect of inflammation condition was studied on erythrocyte exposed to hypotonic solution. In this in vitro method the membrane stabilising action leads to anti-inflammatory activity and was compared with that produced by diclofenac sodium as the reference standard. Results of the evaluation indicate that the synthesised compounds found to exhibit membrane stabilising activity

Issue:4 Citation

ABSTRACT Melamine is a toxic triazine used as an adulterant in milk & milk related products to increase the protein content. RP-HPLC method has been developed using water as mobile phase to detect and quantify the melamine residue in powdered milk. Melamine was extracted by precipitation of milk protein at its isoelectric point with dilute acids. Samples were purified by using membrane filter of 0.45 µm pore size, AmChemteq ACI C 18 (4.6mmx250mm, 5µm) column as the stationary phase. The linearity curve was constructed for the concentration of melamine ranging from 1 mg/kg to 25 mg/kg. The correlation co-efficient was found to be 1.000. Ten marketed powdered milk brands were studied by this method. It was observed that all the ten brands were found to contain melamine residue ranging from 0.00001 mg/kg to 0.00006 mg/kg. The results were below the limits set by Food Safety and Standards Authority of India. This is the first study to confirm the existence of melamine residue in powdered milk marketed in Tamilnadu

Evaluation of In Vitro Anti-inflammatory Activity of Azomethines of Aryl Oxazoles

Ability to inhibit erythrocyte hemolysis is often used as a characteristic of the membrane stabilising action of chemical compounds. Azomethines of aryl oxazoles were evaluated for anti-inflammatory by in vitro hemolytic membrane stabilising study. The effect of inflammation condition was studied on erythrocyte exposed to hypotonic solution. In this in vitro method the membrane stabilising action leads to anti-inflammatory activity and was compared with that produced by diclofenac sodium as the reference standard. Results of the evaluation indicate that the synthesised compounds found to exhibit membrane stabilising activity

IN VITRO ANTIOXIDANT AND CYTOTOXIC ACTIVITY OF ISOLATED COMPOUNDS ON ROOTS OF CLERODENDRUM PHLOMIDIS LINN

 Objective: The main aim of the study was to screen the isolated compounds of Clerodendrum phlomidis roots for its in vitro antioxidant and anticanceractivity and its efficacy against HeLa cell lines.Methods: Pet ether, chloroform, ethyl acetate and ethanol extracts was prepared and assayed for the presence of phytochemicals. Threecompounds were isolated from ethanol extract of C. phlomidis by column chromatography such as ET1 (phenyl acetic acid), ET2 (ethyl-2-hydroxy-4-methylbenzoate), ET3 (3,6,7-trihydroxy-2-(3-methoxyphenyl)-4H-chromen-4-one) characterized by IR (KBr), 1H-nuclear magnetic resonance(NMR), 13C-NMR and Gas chromatography–mass spectrometry. The above isolated compounds were subjected to in vitro antioxidant activity against2,2-diphenylpicrylhydrazyl (DPPH) radical, superoxide radical scavenging assay and iron chelating activity. The effect of isolated compounds on HeLacancer cell line was also evaluated by 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide colorimetric assay.Results: ET2 has good in vitro antioxidant activity against DPPH radical, super oxide radical and iron chelating activity with an inhibitory concentration50% value of 360 μg/ml, 150 μg/ml and 130 μg/ml respectively. ET1 showed significant cytotoxic activity than the other two compounds on HeLacells with a percentage cell growth of 21.9% and a growth inhibition of 50% value of 180 μg, respectively.Conclusion: On the basis of obtained results, ET1 and ET2 isolated from the ethanolic extract of C. phlomidis represent a new group of cytotoxicagainst HeLa cell line and antioxidant agents.Keywords: Column chromatography, Clerodendrum phlomidis, In vitro antioxidant activity, 2,2-diphenylpicrylhydrazyl, Cytotoxicity,3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide assay.Â

GREEN SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES FROM AQUEOUS EXTRACT OF BASELLA ALBA AND THEIR IN-VITRO ANTIOXIDANT POTENTIALS

Objective: To synthesize silver nanoparticles (AgNps) from Basella alba(B. alba) aqueous extract using 3 mM AgNO3 solution, to characterize the resulted AgNps and also to study the antioxidant activity by DPPH and reducing assay. Methods: 150 ml of aqueous extract was mixed with 600 ml of 3 mM silver nitrate solutions and kept at room temperature for 1hr. A change from straw yellow to golden yellow was observed and absorption spectra were obtained by UV-VIS spectrometer. The resulted nanoparticles (NPs) were characterized by FT-IR spectrum, SEM-EDS, TEM and XPS techniques. The purity and nature of the particles were observed through TG-DSC analysis. Further, Antioxidant activity was performed by DPPH (2, 2-diphenyl-1-picrylhydrazyl) method and by Ferric chloride reducing assay. Results: UV-Vis spectral analysis shows a maximum absorption peak at 435 nm. FT-IR spectrum indicated the formation of reducing and capping agents in AgNps. The synthesized silver nanoparticles was spherical in shape and its size ranges from 22.6 to 25 nm. The synthesized silver nanoparticles possess excellent antioxidant activity by various methods. The phenol carboxylic acid present in B. alba leaf extract acted as a reducing agent which was identified by HPTLC technique. A Protein present in the B. alba extract was responsible for the capping of bio-reduced silver nanoparticles. Conclusion: It has been demonstrated that Basella alba plant extract could be used as a proficient green reducing agent for the synthesis of AgNPs. Further studies will be focused towards the mass scale production of formulation