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Sinus histiocytosis with massive lymphadenopathy (Rosai-Dorfman disease) : report of a patient with isolated renotesticular involvement after cure of non-Hodgkin's lymphoma
Intravascular lymphoma associated with haemophagocytic syndrome: a very rare entity in western countries
Prevalence of Hepatitis B Surface Antigen in Pregnant Women in Beheshti Hospital of Kashan, Isfahan
Features of primary extranodal lymphoma in Kanagawa, a human T-cell leukemia virus type 1 nonendemic area in Japan
Isolation and Characterization of CvIV4: A Pain Inducing α- Scorpion Toxin
Ashlee H. Rowe is with UT Austin, Yucheng Xiao is with Indiana University School of Medicine, Joseph Scales is with UT Austin, Klaus D. Linse is with UT Austin, Matthew P. Rowe is with Sam Houston State University, Theodore R. Cummins is with Indiana University School of Medicine, Harold H. Zakon is with UT Austin.Background -- Among scorpion species, the Buthidae produce the most deadly and painful venoms. However, little is known regarding the venom components that cause pain and their mechanism of action. Using a paw-licking assay (Mus musculus), this study compared the pain-inducing capabilities of venoms from two species of New World scorpion (Centruroides vittatus, C. exilicauda) belonging to the neurotoxin-producing family Buthidae with one species of non-neurotoxin producing scorpion (Vaejovis spinigerus) in the family Vaejovidae. A pain-inducing α-toxin (CvIV4) was isolated from the venom of C. vittatus and tested on five Na+ channel isoforms. Principal Findings -- C. vittatus and C. exilicauda venoms produced significantly more paw licking in Mus than V. spinigerus venom. CvIV4 produced paw licking in Mus equivalent to the effects of whole venom. CvIV4 slowed the fast inactivation of Nav1.7, a Na+ channel expressed in peripheral pain-pathway neurons (nociceptors), but did not affect the Nav1.8-based sodium currents of these neurons. CvIV4 also slowed the fast inactivation of Nav1.2, Nav1.3 and Nav1.4. The effects of CvIV4 are similar to Old World α-toxins that target Nav1.7 (AahII, BmK MI, LqhIII, OD1), however the primary structure of CvIV4 is not similar to these toxins. Mutant Nav1.7 channels (D1586A and E1589Q, DIV S3–S4 linker) reduced but did not abolish the effects of CvIV4. Conclusions -- This study: 1) agrees with anecdotal evidence suggesting that buthid venom is significantly more painful than non-neurotoxic venom; 2) demonstrates that New World buthids inflict painful stings via toxins that modulate Na+ channels expressed in nociceptors; 3) reveals that Old and New World buthids employ similar mechanisms to produce pain. Old and New World α-toxins that target Nav1.7 have diverged in sequence, but the activity of these toxins is similar. Pain-inducing toxins may have evolved in a common ancestor. Alternatively, these toxins may be the product of convergent evolution.This work was supported, in part or in whole, by Department of The Army Grants W911NF-06-1-0213 and W911NF-09-1-0355 from the Army Research Office (ARO) Life Sciences Division (to Dr. Rowe and Dr. Zakon) and by National Institutes of Health Ruth L. Kirschstein National Research Service Award Postdoctoral Fellowship from the National Institute of General Medical Sciences (NIGMS) (to Dr. Rowe). This research was also supported by National Institutes of Health Grant NS053422 from the National Institute of Neurological Disorders and Stroke (NINDS) (to Dr. Cummins), and by a University of Texas at Austin College of Natural Sciences Undergraduate Research Fellowship (to Dr. Scales). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Biological Sciences, School o