<i>In vitro</i> cytotoxic effect of LfcinB (20–25)₄.

<p>Structural damage caused by LfcinB (20–25)₄ in CAL27 and HET-1A cell lines was estimated via transmission electron microscope. Images show sections of <b>(A)</b> CAL27 and <b>(B)</b> HET-1A cells, pre- and post- treatments. Scale bar = 2 μm.</p

Degree of leukocyte infiltration in OSCC tumors and quantification of peptide-specific IgG.

<p>Following five weeks of treatment, tumor leukocyte infiltration and the presence of peptide-specific IgG were studied. <b>(A)</b> Sections of the oral buccal pouch stained with H&E, showing the inflammatory infiltrate. Scale bar = 100 μm. <b>(B)</b> Quantitative analysis of the leukocyte infiltrate score: absent (-), mild (+), moderate (++) and severe (+++). <b>(C)</b> Quantitative analysis of the amount of hamster IgG in serum of animals treated with peptides or vehicle. <b>(D)</b> Increase of the levels of peptide-specific hamster IgG relative to normal hamster IgG levels (absorbance at 450 nm). Representative data of 5 animals per group. ^*indicates a significant difference (p < 0.05) relative to the peptide and vehicle treatments.</p

Histological characteristics of OSCC tumors.

<p>Following five weeks of treatment, the histological characteristics of OSCC tumors were evaluated<b>.</b> Images show sections of the oral buccal pouch stained with H&E, emphasizing the stroma area. Histological analysis showed acellularity in the stroma of animals treated with LfcinB(20–25)₄ and LfcinB(20–25). Scale bar = 100 μm. </p

Bovine Lactoferricin-derived peptides used in this study.

<p>Bovine Lactoferricin-derived peptides used in this study.</p