Prion protein in the cerebrospinal fluid of healthy and naturally scrapie-affected sheep

The aim of this study was to characterize the cerebrospinal fluid (CSF) prion protein (PrP) of healthy and naturally scrapie-affected sheep. The soluble form of CSF PrPC immunoblotted with an anti-octarepeat and an anti-C terminus mAb showed two isoforms of approximately 33 and 26 kDa, corresponding to the biglycosylated and unglycosylated isoforms of brain PrPC. Neither the mean concentration nor the electrophoretic profile of CSF PrP differed between healthy and scrapieaffected sheep, whereas a slightly increased resistance of CSF PrP to mild proteolysis by proteinase K was evident in the CSF of scrapie-affected sheep. No difference in susceptibility to proteolysis was observed between the two ARR and VRQ genetic variants of the purified prokaryote recombinant PrP. It was concluded that the physicochemical properties of PrPC in the CSF could be altered during scrapie and that these changes might reflect the physiopathological process of prion disease

Biodegradation of PAHs by Trametes versicolor in presence of montanox

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Influence of adsorption-desorption phenomena on pesticide runoff measured under controlled conditions

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Relations entre les distributions granulometriques par granulométrie laser et par analyse d'images en microscopie électronique à transmission de la fraction argileuse d'un sol

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Influence of the formulation on the sorption and the mobility of diuron in soil

*INRA Unité de Phytopharmacie et Médiateurs Chimiques Versailles (FRA) Diffusion du document : INRA Unité de Phytopharmacie et Médiateurs Chimiques Versailles (FRA)International audienc

Dispositif "42 parcelles-Versailles" et adsorption des pesticides

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Heterogeneous dissolution of benzo(a)pyrene by surfactant solutions

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Transport pathways for viruses in a sandstone aquifer

Following the discovery of viable human enteric viruses at depth in a sandstone aquifer in the U.K., a set of tracer experiments was conducted between March 2004 and August 2005 at a test site in a similar sandstone aquifer, using bacteriophages as human virus surrogates. Initial tests showed a range of bacteriophages (PRD1, φX174, H40/1 and MS2) to be transported between two 50 m boreholes, but attempts to identify the transport pathway(s) by tracer tests in packered horizons between boreholes proved unsuccessful. A new inter-borehole tracer test with injection at one borehole and abstraction from a pumping borehole has now been designed with novel instrumentation to identify the lithological horizons transporting viruses. In addition to the detailed descriptions of core available, the array of boreholes has been subjected to extensive geophysical logging (resistivity, natural gamma, optical televiewer) and hydraulic testing (constant discharge tests, single-hole and cross-hole packer tests). On the basis of these data a detailed conceptualisation of the site has been constructed, with a number of hydraulically significant low permeability horizons clearly identified. The new instrumentation allows the viruses and a conservative tracer to be sampled at different horizons in the abstraction borehole while the hole is being pumped. Viruses are enumerated by plaque assay an