Historical basins sediments

Modifications of small water reservoirs in the past focused primarily on addressing issues of capacity and stability of the reservoir capture. By the impact of changes in the use of reservoir surroundings, by acceleration of the erosion processes on the adjecent land and non-periodical maintenance of the capture leads to morphological changes in the reservoir, which causes changes in the in the reduced flowage of the basin and thus in changes of the flood protection degree of adjacent land. This reduces the accumulation part of the basin and subsequently its economic use in fish rearing Bottom sediments of dams are products of erosion of agricultural and forest soils, the main flow of the river basin and tributaries connected to the dam or a system of dams. They have the basic properties of surface layers of soil erosion

Point of care colourimetric and lateral flow LAMP assay for the detection of

In this study, we present an optimised colourimetric and a lateral flow LAMP assay for the detection of Haemonchus contortus in small ruminant faecal samples. Using a previously published LAMP primer set, we made use of commercially available colourimetric LAMP and lateral flow kits and combined this into an optimised diagnostic assay which was then tested on field faecal samples from Eastern and South-Eastern Hungary as well as a pure H. contortus egg faecal sample from Košice, Slovakia. Both assays showed no conflicts in visual detection of the results. Additionally, we modified and tested several centrifuge-free DNA extraction methods and one bead-beating egg lysis DNA extraction method to develop a true point of care protocol, as the source of the starting DNA is the main rate-limiting step in farm-level molecular diagnosis. Out of the various methods trialed, promising results were obtained with the magnetic bead extraction method. Sample solutions from the Fill-FLOTAC® technique were also utilised, which demonstrated that it could be efficiently adapted for field-level egg concentration to extract DNA. This proof of concept study showed that isothermal amplification technologies with a colourimetric detection or when combined with a lateral flow assay could be an important step for a true point of care molecular diagnostic assay for H. contortus