ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF HEDYOTIS CORYMBOSA, INULA RACEMOSE, HOLOSTEMMA ADAKODIEN MEDICINAL PLANTS

Objective: To evaluate the antibacterial and antifungal activity of three different medicinal plants invitro. Oil extracts of the plants from three different families (Hedyotis corymbosa, Inula racemose, Holostemma adakodien) are used to find the antibacterial and antifungal activity. Methods: Study of anti-microbial activity using agar cup diffusion method, inoculums preparation, Anti-bacterial activity screening (well diffusion method), Anti-fungal activity screening (well diffusion method) are the different methods which are used to find the anti-bacterial and anti-fungal activities of the oil extracts of the above medicinal plants. The activity of these oil extracts and the selected antibiotics are evaluated against four bacterial strains (E. Coli, S. aureus and P. aeruginosa) and three Candida fungal strains (albicans, parapsilosis and tropicalis.). The anti microbial activity of Hedyotis corymbosa, Inula racemose, Holostemma adakodien oil extracts zone of inhibition evaluation was compared with positive control of fungal strain Fluconozole and bacterial strain Norfloxacin, Cefepime and Gatifloxacin. Results: The anti microbial activity of Hedyotis corymbosa, Inula racemose, Holostemma adakodien oil extracts zone of inhibition evaluation was compared with positive control of fungal strain Fluconozole and bacterial strain Norfloxacin, Cefepime and Gatifloxacin. Further studies are needed to explore the novel antibacterial bioactive molecules. Conclusion: The plants Hedyotis corymbosa, Inula racemose and Holostemma adakodien have shown significant anti-bacterial and anti-fungal activity

ANTIPYRETIC ACTIVITY OF ANNONA PLANTS LEAVES ON BREWER'S YEAST INDUCED FEBRILE RATS

Objective: To evaluate and compare the in vivo antipyretic activity of the methanolic extracts of selected three Annona plant leaves.Methods: The acute oral toxicity determined by OECD class method and the in vivo antipyretic activity was determined by brewer's yeast inducedpyrexia method.Results: The results showed that the methanolic extract of leaves of Annona plants are non-toxic and possessed significant antipyretic effect whichmay be attributed to the presence of flavonoids and saponins in the extracts.Conclusion: This study provides evidences for the antipyretic activity of Annona squamosa, Annona reticulata and Annona muricata possess antipyreticactivity at the tested doses 100 and 200 mg/kg body weight without any side effects, which could partly contribute to its ethno medical use.Keywords: Annona squamosa L, Annona reticulata L, Annona muricata L, Antipyretic activity, Brewer's yeast