NetSci High: Bringing Agency to Diverse Teens Through the Science of Connected Systems

This paper follows NetSci High, a decade-long initiative to inspire teams of teenage researchers to develop, execute and disseminate original research in network science. The project introduced high school students to the computer-based analysis of networks, and instilled in the participants the habits of mind to deepen inquiry in connected systems and statistics, and to sustain interest in continuing to study and pursue careers in fields involving network analysis. Goals of NetSci High ranged from proximal learning outcomes (e.g., increasing high school student competencies in computing and improving student attitudes toward computing) to highly distal (e.g., preparing students for 21st century science), with an emphasis on doing real-world research into relevant and ambiguous problems through technologically-infused and highly collaborative projects and defending them to a clear (and sometimes potentially intimidating) audience. The cognitive goals of the project covered broad areas including analyzing, synthesizing, and visualizing quantitative data, and understanding modeling and network statistics. Attitudinal outcomes included improving attitudes toward the statistical study of networks, self-efficacy, and a sense of agency for continuing to pursue further involvement in college studies and careers

Genetic analysis for sooty mold resistance and heart of palm yield in Archontophoenix.

Palmeiras do gênero Archontophoenix, utilizadas tanto como ornamentais quanto produtoras de palmito de qualidade, são susceptíveis à fumagina, doença associada à infestação por pulgões, que afeta a fotossíntese, o crescimento e a aparência das plantas. Foram avaliados a campo a resistência à fumagina conjuntamente com três caracteres associados ao crescimento em 24 famílias de meios-irmãos, 28 meses após o plantio, a fim de identificar a variabilidade genética para os caracteres resistência à fumagina, altura, diâmetro e número de folhas; estimar as correlações genotípicas e fenotípicas envolvendo esses quatro caracteres; e aplicar a estratégia de seleção usando o índice multiefeitos. Houve diferenças entre as famílias para os caracteres avaliados, sugerindo a possibilidade de seleção. O baixo coeficiente de variação observado para resistência à fumagina (9,48%) indica que o método de avaliação adotado, baseado em escala de notas após observação visual, foi eficiente e prático para comparar níveis de infestação do complexo fungo+pulgão em palmeiras do gênero Archontophoenix. As estimativas da herdabilidade no sentido restrito foram baixas a médias para os caracteres relacionados ao crescimento (0,10, 0,26 e 0,26 para número de folhas, diâmetro e altura da planta, respectivamente) e muito altas (0,91) para resistência à fumagina. Correlação genética positiva foi observada entre resistência à fumagina e altura da planta, indicando que a eliminação de plantas muito susceptíveis pode ser feita sem interferência na seleção indireta para produção de palmito. A estratégia de seleção pelo índice multiefeitos (com ganhos genéticos esperados variando de 6,23 a 11,83%) mostrou-se adequada para melhorar simultaneamente caracteres relacionados ao crescimento e à produção de palmito

Immunohistochemical expression of apoptotic factors, cytokeratins, and metalloproteinase-9 in periapical and epithelialized gingival lesions

The aim of the study was to assess the involvement of apoptotic factors, cytokeratins and metalloproteinase- 9 in the histogenesis of both Epithelialized Gingival Lesions (EGL) and Periapical Lesions (PAL). 55 consecutive patients, 30 with PAL and 25 with EGL, were selected for the study after clinical and radiological examinations. The PAL patients had severe periapical lesions and tooth decay with exposure of the pulp chamber. All PAL and EGL biopsies were surgically extracted, fixed in 10% buffered formalin, and processed for routine light microscopy. Ten biopsies of each category were processed for immunohistochemistry (IHC). Serial paraffin sections were stained by IHC with appropriate antibodies to detect cytokeratins (CKs) 1, 5, 8, 10 and 14, caspase-3 and -9, metalloproteinase-9, and for PCNA and TUNEL assays. Both PAL and EGL showed a high expression of the cytokeratin 1, 5 and 8 with higher expression in EGL. Moreover, CK10 was markedly less intense expressed in EGL compared to PAL, while CK14 was almost three times stronger expressed in EGL. The expression of caspase-3 and -9 was stronger in PAL compared to EGL, however, the difference was only significant for caspase-9. In PAL apoptosis detected by TUNNEL method and the expression of MMP-9 were higher than in EGL, whereas PCNA was significantly more expressed in EGL. The results clearly suggest that both lesions have exclusively an epithelial origin and that epithelial proliferation was correlated with the degree of apoptosis in both entities. PAL and EGL presented mostly similar cytokeratin expression except for CK10 and CK14, though with marked differences in the distribution and intensity of IHC reactions. Finally, the degradation of extracellular matrix in both lesions could be partially attributed to the strong presence of MMP-9. (Folia Histochemica et Cytobiologica 2012, Vol. 50, No. 4, 497\u2013503

Cadmium down-regulates expression of XIAP at the post-transcriptional level in prostate cancer cells through an NF-κB-independent, proteasome-mediated mechanism

<p>Abstract</p> <p>Background</p> <p>Cadmium has been classified as a human carcinogen, affecting health through occupational and environmental exposure. Cadmium has a long biological half-life (>25 years), due to the flat kinetics of its excretion. The prostate is one of the organs with highest levels of cadmium accumulation. Importantly, patients with prostate cancer appear to have higher levels of cadmium both in the circulation and in prostatic tissues.</p> <p>Results</p> <p>In the current report, we demonstrate for the first time that cadmium down-regulates expression of the X-linked inhibitor of apoptosis protein (XIAP) in prostate cancer cells. Cadmium-mediated XIAP depletion occurs at the post-transcriptional level via an NF-κB-independent, proteasome-mediated mechanism and coincides with an increased sensitivity of prostate cancer cells to TNF-α-mediated apoptosis. Prolonged treatment with cadmium results in selection of prostate cancer cells with apoptosis-resistant phenotype. Development of apoptosis-resistance coincides with restoration of XIAP expression in cadmium-selected PC-3 cells.</p> <p>Conclusions</p> <p>Selection of cadmium-resistant cells could represent an adaptive survival mechanism that may contribute to progression of prostatic malignancies.</p

The WHI Corona from Differential Emission Measure Tomography

A three dimensional (3D) tomographic reconstruction of the local differential emission measure (LDEM) of the global solar corona during the whole heliosphere interval (WHI, Carrington rotation CR-2068) is presented, based on STEREO/EUVI images. We determine the 3D distribution of the electron density, mean temperature, and temperature spread, in the range of heliocentric heights 1.03 to 1.23 Rsun. The reconstruction is complemented with a potential field source surface (PFSS) magnetic-field model. The streamer core, streamer legs, and subpolar regions are analyzed and compared to a similar analysis previously performed for CR-2077, very near the absolute minimum of the Solar Cycle 23. In each region, the typical values of density and temperature are similar in both periods. The WHI corona exhibits a streamer structure of relatively smaller volume and latitudinal extension than during CR-2077, with a global closed-to-open density contrast about 6% lower, and a somewhat more complex morphology. The average basal electron density is found to be about 2.23 and 1.08 x 10^8 cm^-3, in the streamer core and subpolar regions, respectively. The electron temperature is quite uniform over the analyzed height range, with average values of about 1.13 and 0.93 MK, in the streamer core and subpolar regions, respectively. Within the streamer closed region, both periods show higher temperatures at mid-latitudes and lower temperatures near the equator. Both periods show beta>1 in the streamer core and beta<1 in the surrounding open regions, with CR-2077 exhibiting a stronger contrast. Hydrostatic fits to the electron density are performed, and the scale height is compared to the LDEM mean electron temperature. Within the streamer core, the results are consistent with an isothermal hydrostatic plasma regime, with the temperatures of ions and electrons differing by up to about 10% .. (continues)..Comment: 13 Figure

A Prospective Observational Study on the Role of Immunohistochemical Expression of Orphanin in Laryngeal Squamous Cell Carcinoma Recurrence

To date, histological biomarkers expressed by laryngeal cancer are poorly known. The identification of biomarkers associated with laryngeal squamous cell carcinoma (SCC), would help explain the tumorogenesis and prevent the possible recurrence of the lesion after treatment. For this reason, the aim of this study is to investigate, for the first time, the Orphanin expression in 48 human specimens of laryngeal SCC and evaluate its possible correlation with patients prognosis. We analyzed pathological specimens from 48 patients with laryngeal SCC to detect the presence of Orphanin by using an immunohistochemistry test. We compared the findings with healthy tissue acquired from patients who underwent surgery for mesenchymal benign tumours of the larynx. The specimens were stained with anti-Orphanin monoclonal antibodies. Results were processed through a computerised image analysis system to determine a scale of staining intensity. All the tumoural specimens examined showed a significant immunoreaction for Orphanin when compared with healthy tissues (p &lt; 0.05) but with a different immune reactivity related to clinical-pathological features. A high Orphanin expression was not significantly related to Histological Grading (HG), TNM, and stage (p &gt; 0.05). In the multivariate analysis, the Orphanin expression was significantly related only to the malignant recurrence (p &lt; 0.05). Our study suggests that Orphanin could have a role in tumorigenesis by increasing the recurrence of cancer; therefore, it should be further explored as a possible biomarker for laryngeal cancer