Designing and Developing Dynamic Decision Support Information for Disaster Response

Recently, Japan has been struck by extremely heavy rains and serious floods. To plan for disaster response, it is needed to consider various information such as precipitation, water level of rivers, weather forecasts, and so on. However, it requires high skill to integrate this information to utilize. Our study aims to provide the decision support information for both the national and the local governments by dynamic risk analysis to enforce disaster resilience. To demonstrate the effectiveness of disaster dynamics analysis, we have developed the “Dynamic Decision Support System for Disaster Response (DDS4D)”. DDS4D synthesizes natural observation data, social observation data, and geospatial data to provide decision support information that fits decision maker's the situational awareness in real time. Verifying several information products generated by DDS4D in the actual flood in Japan, we confirmed that they could support decision making of government.</p

Single nucleotide polymorphisms in surfactant protein A1 are not associated with a lack of responsiveness to antenatal steroid therapy in a pregnant sheep model

Treatment with antenatal steroids (ANS) is standard practice for reducing the risk of respiratory distress in the preterm infant. Despite clear overall benefits when appropriately administered, many fetuses fail to derive benefit from ANS therapies. In standardized experiments using a pregnant sheep model, we have demonstrated that around 40% of ANS-exposed lambs did not have functional lung maturation significantly different from that of saline-treated controls. Surfactant protein A is known to play an important role in lung function. In this genotyping study, we investigated the potential correlation between polymorphisms in SFTPA1, messenger RNA and protein levels, and ventilation outcomes in animals treated with ANS. 45 preterm lambs were delivered 48 h after initial ANS therapy and 44 lambs were delivered 8 days after initial ANS therapy. The lambs were ventilated for 30 min after delivery. SFTPA1 mRNA expression in lung tissue was not correlated with arterial blood PaCO2 values at 30 min of ventilation in lambs delivered 48 h after treatment. SFTPA1 protein in lung tissue was significantly correlated with PaCO2 at 30 min of ventilation in lambs ventilated both 48 h and 8 days after ANS treatment. Six different single nucleotide polymorphisms (SNPs) in the Ovis aries SFTPA1 sequence were detected by Sanger Sequencing. No individual SNPs or SNP haplotypes correlated with alterations in PaCO2 at 30 min of ventilation or SFTPA1 protein levels in the lung. For the subset of animals analyzed in the present study, variable lung maturation responses to ANS therapy were not associated with mutations in SFTPA1

Preparation of atomically clean and flat Si(100) surfaces by low-energy ion sputtering and low-temperature annealing

Si(100) surfaces were prepared by wet-chemical etching followed by 0.3-1.5keV Ar ion sputtering, either at elevated or room temperature. After a brief anneal under ultrahigh vacuum conditions, the resulting surfaces were examined by scanning tunneling microscopy. We find that wet-chemical etching alone cannot produce a clean and flat Si(100) surface. However, subsequent 300eV Ar ion sputtering at room temperature followed by a 973K anneal yields atomically clean and flat Si(100) surfaces suitable for nanoscale device fabrication.Comment: 13 pages, 3 figures, to be published in Applied Surface Scienc

Modelling of molecular genetic systems in bacterial cell 45 AROMATIC AMINO ACID BIOSYNTHESIS IN ESCHERICHIA COLI: GENERALIZED HILL FUNCTION MODEL OF THE TRYPTOPHAN- SENSITIVE 3-DEOXY-D-ARABINO- HEPTULOSONATE-7-PHOSPHATE SYNTHASE REACTION DEMONSTRATE COMPL

SUMMARY Motivation: Development of an in silico cell as a computer resource for simulation and analysis of processes within living cells is an urgent task of systems biology and computational biology. Results: By using the GeneNet technology, we reproduced the gene network of the regulation of aromatic amino acid biosynthesis in the E. coli cell. Mathematical models were constructed by the method of generalized Hill functions. The models describe the efficiency of enzymatic systems and regulation of expression of related genes. Mathematical model of the enzyme tryptophan-sensitive 3-deoxy-d-arabinoheptulosonate-7-phosphate synthase reaction demonstrate complicated mechanism. Availability: Models are available on request. The diagram of the gene network regulating aromatic amino acid biosynthesis in E. coli is available through the GeneNet viewer a

Betamethasone phosphate reduces the efficacy of antenatal steroid therapy and is associated with lower birth weights when administered to pregnant sheep in combination with betamethasone acetate

Background Antenatal corticosteroid (ACS) therapy is standard of care for women at imminent risk of preterm labour. Despite this, much remains to be understood regarding an optimal (maximum benefit, minimal risk of side effects) ACS dosing strategy. Although conveying overall benefit when given to the right patient at the right time, ACS treatment efficacy is highly variable, and is not risk-free. Building on earlier findings, we hypothesized that when administered in combination with slow-release betamethasone acetate, betamethasone phosphate and the high materno-fetal betamethasone concentrations it generates are redundant for fetal lung maturation. Objective Using an established sheep model of prematurity and post-natal ventilation of the preterm lamb, we aimed to compare the pharmacodynamic effects of a low-dose treatment with betamethasone acetate only against a standard dose of betamethasone phosphate and betamethasone acetate as recommended by the American College of Obstetricians and Gynaecologists for women at risk of imminent preterm delivery between 24 and 35+6 weeks’ gestation. Methods Ewes carrying a single fetus at 122±1 d gestational age (term=150d) were randomized to receive either: i) maternal intramuscular injections of sterile saline (the Saline Negative Control Group, n=12), ii) two maternal intramuscular injections of 0.25 mg/kg betamethasone phosphate + acetate spaced by 24h (the Beta-P+Ac Group, n=12); or iii) two maternal intramuscular injections of 0.125 mg/kg betamethasone acetate spaced by 24h (the Beta-Ac Group, n=11). Fetuses were surgically delivered 48h after treatment initiation and ventilated for 30 minutes to determine functional lung maturation. Fetuses were euthanized after ventilation and lung were collected for analysis using quantitative polymerase chain reaction and western blot assays. Fetal plasma ACTH levels were measured in the cord blood samples taken at delivery. Results Preterm lambs were defined as either ACS treatment responders or non-responders using an arbitrary cut-off, being a PaCO2 level at 30 minutes of ventilation being more extreme than two standard deviations from the mean value of the normally-distributed Saline Control Group values. Relative to Saline Control Group animals, both ACS treatment group animals showed significantly improved lung physiological responses (blood gas and ventilation data) and had a biochemical signature (mRNA and surfactant protein assays) consistent with functional maturation. However, the Beta-Ac Group had a significantly higher treatment response rate than the Beta-P+Ac Group. These physiological results were strongly correlated to the amount of surfactant protein A. Birth weight was lower in Beta-P+Ac Group and the fetal HPA axis was supressed to a greater extent in the Beta-P+Ac Group. Conclusion Low dose ACS therapy solely employing Beta-Ac was sufficient for fetal lung maturation. The elevated materno-fetal betamethasone concentrations associated with the co-administration of betamethasone phosphate did not additionally improve lung maturation, but were associated with greater HPA axis suppression, a lower ACS treatment response rate, and lower birth weight – outcomes not desirable in a clinical setting. These data warrant a clinical investigation of sustained, low-dose ACS treatments that avoid high materno-fetal betamethasone exposures

Massive star-formation toward G28.87+0.07 (IRAS 18411-0338) investigated by means of maser kinematics and radio to infrared, continuum observations

We used the Very Long Baseline Array (VLBA) and the European VLBI Network (EVN) to perform phase-referenced VLBI observations of the three most powerful maser transitions associated with the high-mass star-forming region G28.87+0.07: the 22.2 GHz H$_{2}$O, 6.7 GHz CH$_{3}$OH, and 1.665 GHz OH lines. We also performed VLA observations of the radio continuum emission at 1.3 and 3.6 cm and Subaru observations of the continuum emission at 24.5 $\mu$m. Two centimeter continuum sources are detected and one of them (named "HMC") is compact and placed at the center of the observed distribution of H$_{2}$O, CH$_{3}$OH and OH masers. The bipolar distribution of line-of-sight (l.o.s) velocities and the pattern of the proper motions suggest that the water masers are driven by a (proto)stellar jet interacting with the dense circumstellar gas. The same jet could both excite the centimeter continuum source named "HMC" (interpreted as free-free emission from shocked gas) and power the molecular outflow observed at larger scales -- although one cannot exclude that the free-free continuum is rather originating from a hypercompact \ion{H}{2} region. At 24.5 $\mu$m, we identify two objects separated along the north-south direction, whose absolute positions agree with those of the two VLA continuum sources. We establish that $\sim$90% of the luminosity of the region ($\sim$\times10^{5} L_\sun$) is coming from the radio source "HMC", which confirms the existence of an embedded massive young stellar object (MYSO) exciting the masers and possibly still undergoing heavy accretion from the surrounding envelope.Comment: Accepted for publication in Ap