COMPARATIVE STUDY OF Î’-CYCLODEXTRIN PRODUCTION FROM THE CGTASE PRODUCING NOVEL STRAINS- ITS CHARACTERIZATION AND HOST-GUEST COMPLEX FORMATION

The enzymatic synthesis of β-CD by CGTase produced from different strains of alkaliphilic bacterial culture which was isolated from the cultivated sugarcane fields and standard MTCC cultures using starch as substrate. An alkalophilic bacteria were grown for six days at static conditions at pH 10.5. The time course of CGTase activity was studied with the maximum activity observed on 6th day. The activity check and its confirmation were done by Dextrinising and Phenolphthalein assay. The soluble starch was best substrate to produce the cyclodextrin. Extraction of β-CD was done using complexing agents, these agents binds only to β-CD and forms complex, the complex thus formed was recovered. The obtained β-CD was made inclusion complex with guest molecule and was further characterized using UV absorption spectrophotometer, FT-IR and melting point.   Keywords: Cyclodextrin glycosyltransferases, Cyclodextrins, Bacillus licheniformis