Camelina Oil Supplementation Improves Bone Parameters in Ovariectomized Rats

The aim of the present study was to determine the effect of administration of Camelina sativa oil (CO) as a source of polyunsaturated fatty acids (PUFA) on bone parameters in ovariectomized rats (OVX). Overall, 40 10-week-old healthy female Wistar rats were divided into 4 groups with 10 animals in each. Rats in the control group (SHO) were subjected to a sham operation, whereas experimental rats (OVX) were ovariectomized. After a 7-day recovery period, the SHO the rats received orally 1 mL of physiological saline for the next 6 weeks. The OVX rats received orally 1 mL of physiological saline (OVX-PhS), 5 g/kg BW (OVX-CO5), or 9 g/kg BW (OVX-CO9) of camelina oil. The use of camelina oil had a significant effect on body weight, lean mass, and fat mass. The camelina oil administration suppressed the decrease in the values of some densitometric, tomographic, and mechanical parameters of femur caused by estrogen deficiency. The CO treatment increased significantly the serum level of osteocalcin and decreased the serum level of C-terminal telopeptide of type I collagen in the OVX rats. In conclusion, camelina oil exerts a positive osteotropic effect by inhibiting ovariectomy-induced adverse changes in bones. Camelina oil supplementation can be used as an efficient method for improving bone health in a disturbed state. However, further research must be carried out on other animal species supplemented with the oil

Age-Related Changes in Acute Phase Reaction, Cortisol, and Haematological Parameters in Ewes in the Periparturient Period

A well-functioning immune system is the basis for protection against infectious and metabolic diseases, and a smooth return to homeostasis. The periparturient period is considered critical because major changes in the endocrine, behavioural, digestive, and immune systems dysregulate immune function, leading to immunosuppression. With age, the immune system could become dysregulated. The purpose of the present investigation was to compare changes in plasma concentrations of acute phase proteins, cortisol, and haematological parameters in the peripheral blood of two age-related groups of healthy ewes to get a better understanding of changes around lambing. Two groups of ewes were enrolled in the study: 3-year-old (young; n = 9) and 7-year-old ewes (old; n = 9). All females were synchronised and inseminated. In blood plasma, serum amyloid A (SAA) and cortisol concentrations were measured using ELISA tests, a spectrophotometric method to determine haptoglobin (Hp), and a thrombin clottable estimation to determine the fibrinogen (Fb) concentration. The blood parameters were examined using an automated haematological analyser. In clinically healthy ewes, no significant effect of age was observed in SAA, Hp, Fb and cortisol concentration in most of analysed terms. SAA, Hp, Fb, and cortisol fluctuations typical for the periparturient period were observed. There were no age-associated differences in red or white blood cell parameters

Interleukin-6 as a Milk Marker of Clinical and Subclinical Intramammary Infections (IMI) in Cows Caused by <i>Streptococcus</i> spp.

The aim of the study was to evaluate the concentrations of Interleukin-6 (IL-6) in milk and serum of healthy cows (HE) and cows with mastitis caused by Streptococcus spp. The blood and milk samples were obtained from Holstein-Friesian cows (Lublin region, Poland). A total of 43 milk and serum samples from 28 cows with mastitis and 15 healthy cows were selected for study. IL-6 levels in milk from HE cows ranged from 6.09–80.24 pg/mL (median 26.6 pg/mL) and were significantly lower than in milk from both cows with clinical and subclinical mastitis (487.09 pg/mL vs. 26.6 pg/mL in CM, p p Streptococcus spp. develop a local immune response in the mammary gland in response to the pathogen. Monitoring of IL-6 levels in milk can allow early detection of mastitis, which is especially important in cases of subclinical inflammation

Cytokine inflammatory response in dairy cows with mastitis caused by Streptococcus agalactiae

The aim of the study was evaluation of the concentrations of interleukin (IL)-1β, IL-8, IL-12β and tumour necrosis factor alpha (TNF-α) in the serum and milk of cows with mastitis caused by Streptococcus agalactiae