Manfaat Wisata Alam Danau Wisata Sejarah Sebaddang Kecamatan Sebawi Kabupaten Sambas

Danau Wisata Sejarah Sebaddang (DWSS) is one of the tourist attractions located in Sambas district, but most of the environmental services offered do not yet have a market value. Hence the need for an approach to determine the economic value of the benefits obtained in order to estimate the magnitude of demand and consumer surplus. The purpose of this study was to determine the benefits of recreation and makes predictions collecting admission price fixing. To achieve the purpose of the study used the Travel Cost Method. The object of research was the visitors who come to the DWSS. Recreation demand equation Y = 52,906 - 0,001X. At the ticket price Rp.6000,- /person has Rp.4.081,- consumer surplus value, visits to the value of the average willingness to pay Rp.10.081, - /person and the value of the economic benefits of Rp.72.772.392,- /year. This means that if the manager wants to improve tourist facilities it should at least be set ticket prices Rp.10.081,- /person. Key words : Sebaddang lake, benefit, natural recreatio

Pendekatan Kolaboratif dalam Pembelajaran Tematik Terpadu di Kelas III Sekolah Dasar Negeri 21 Kuala Mandor B

: This is a research about collaborative approach in integrated thematic learning on the third grade students of elementary school number 21 Kuala Mandor B. The research problems was how the design, implementation, evaluation and behavior of the students in the integrated thematic learning using collaborative approach on the third grade students of elementary school number 2 Kuala Mandor B. The purpose was to describe the design, implementation, evaluation and behavior of the students in the integrated thematic learning using collaborative approach on the third grade students of elementary school number 2 Kuala Mandor B. The results were (1) thematic lesson plan using collaborative approach done by the teacher was fulfil the thematic lesson plan acquirement in general; (2) thematic learning using collaborative was done according to the design and using varied method and strategy of learning; (3) the evaluation was not monotonous, and was used multiple assessment instrument; (4) students\u27 learning behavior in solving the various problem could be seen through the application of thematic learning using collaborative approach

Androgen Receptor Mutations Associated with Androgen Insensitivity Syndrome: A High Content Analysis Approach Leading to Personalized Medicine

Androgen insensitivity syndrome (AIS) is a rare disease associated with inactivating mutations of AR that disrupt male sexual differentiation, and cause a spectrum of phenotypic abnormalities having as a common denominator loss of reproductive viability. No established treatment exists for these conditions, however there are sporadic reports of patients (or recapitulated mutations in cell lines) that respond to administration of supraphysiologic doses (or pulses) of testosterone or synthetic ligands. Here, we utilize a novel high content analysis (HCA) approach to study AR function at the single cell level in genital skin fibroblasts (GSF). We discuss in detail findings in GSF from three historical patients with AIS, which include identification of novel mechanisms of AR malfunction, and the potential ability to utilize HCA for personalized treatment of patients affected by this condition

Pelatihan Peningkatan Keterampilan Belajar Peserta Didik Menggunakan Aplikasi Canva di Sekolah Perbatasan Indonesia-Malaysia

This service activity is to improve students' learning skills using the Canva application at SMA Negeri 1 Jagoi Babang, Bengkayang Regency (Indonesia – Malaysia Border School). The method of implementing this service uses training and assistance in making products. The evaluation instrument uses survey percentages and is analyzed descriptively. The results of this service show that training activities using the Canva application provide positive benefits and impacts for students, where students can understand the Canva application as an application that can support it in learning activities. Students are able to use this application and produce output in the form of design products, one of which is presentation material

Analysis of Linear Antibody Epitopes on Factor H and CFHR1 Using Sera of Patients with Autoimmune Atypical Hemolytic Uremic Syndrome

Introduction: In autoimmune atypical hemolytic uremic syndrome (aHUS), the complement regulator factor H (FH) is blocked by FH autoantibodies, while 90% of the patients carry a homozygous deletion of its homolog complement FH-related protein 1 (CFHR1). The functional consequence of FH-blockade is widely established; however, the molecular basis of autoantibody binding and the role of CFHR1 deficiency in disease pathogenesis are still unknown. We performed epitope mapping of FH to provide structural insight in the autoantibody recruitment on FH and potentially CFHR1. Methods: Eight anti-FH positive aHUS patients were enrolled in this study. With overlapping synthetic FH and CFHR1 peptides, we located the amino acids (aa) involved in binding of acute and convalescence stage autoantibodies. We confirmed the location of the mapped epitopes using recombinant FH domains 19-20 that carried single-aa substitutions at the suspected antibody binding sites in three of our patients. Location of the linear epitopes and the introduced point mutations was visualized using crystal structures of the corresponding domains of FH and CFHR1. Results: We identified three linear epitopes on FH (aa1157-1171; aa1177-1191; and aa1207-1226) and one on CFHR1 (aa276-290) that are recognized both in the acute and convalescence stages of aHUS. We observed a similar extent of autoantibody binding to the aHUS-specific epitope aa1177-1191 on FH and aa276-290 on CFHR1, despite seven of our patients being deficient for CFHR1. Epitope mapping with the domain constructs validated the location of the linear epitopes on FH with a distinct autoantibody binding motif within aa1183-1198 in line with published observations. Summary: According to the results, the linear epitopes we identified are located close to each other on the crystal structure of FH domains 19-20. This tertiary configuration contains the amino acids reported to be involved in C3b and sialic acid binding on the regulator, which may explain the functional deficiency of FH in the presence of auto antibodies. The data we provide identify the exact structures involved in autoantibody recruitment on FH and confirm the presence of an autoantibody binding epitope on CFHR1.Peer reviewe

Bexarotene: a Novel Modulator of AURKA and the Primary Cilium in VHL-deficient Cells

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Novel GLP-1 Fusion Chimera as Potent Long Acting GLP-1 Receptor Agonist

GLP-1 has a variety of anti-diabetic effects. However, native GLP-1 is not suitable for therapy of diabetes due to its short half-life (t1/2<2 min). To circumvent this, we developed a long-lasting GLP-1 receptor agonist by the fusion of GLP-1 with human IgG2 Fc (GLP-1/hIgG2). ELISA-based receptor binding assay demonstrated that GLP-1/hIgG2 had high binding affinity to the GLP-1R in INS-1 cells (Kd = 13.90±1.52 nM). Upon binding, GLP-1/hIgG2 was rapidly internalized by INS-1 cells in a dynamin-dependent manner. Insulin RIA showed that GLP-1/IgG2 dose-dependently stimulated insulin secretion from INS-1 cells. Pharmacokinetic studies in CD1 mice showed that with intraperitoneal injection (i.p.), the GLP-1/hIgG2 peaked at 30 minutes in circulation and maintained a plateau for >168 h. Intraperitoneal glucose tolerance test (IPGTT) in mice showed that GLP-1/hIgG2 significantly decreased glucose excursion. Furthermore, IPGTT performed on mice one week after a single drug-injection also displayed significantly reduced glucose excursion, indicating that GLP-1/hIgG2 fusion protein has long-lasting effects on the modulation of glucose homeostasis. GLP-1/hIgG2 was found to be effective in reducing the incidence of diabetes in multiple-low-dose streptozotocin-induced type 1 diabetes in mice. Together, the long-lasting bioactive GLP-1/hIgG2 retains native GLP-1 activities and thus may serve as a potent GLP-1 receptor agonist

Homology modeling and molecular dynamics simulations of MUC1-9/H-2Kb complex suggest novel binding interactions

International audienceHuman MUC1 is over-expressed in human adenocarcinomas and has been used as a target for immunotherapy studies. The 9-mer MUC1-9 peptide has been identified as one of the peptides which binds to murine MHC class I H-2K. The structure of MUC1-9 in complex with H-2K has been modeled and simulated with classical molecular dynamics, based on the x-ray structure of the SEV9 peptide/H-2K complex. Two independent trajectories with the solvated complex (10 ns in length) were produced. Approximately 12 hydrogen bonds were identified during both trajectories to contribute to peptide/MHC complex, as well as 1-2 water mediated hydrogen bonds. Stability of the complex was also confirmed by buried surface area analysis, although the corresponding values were about 20% lower than those of the original x-ray structure. Interestingly, a bulged conformation of the peptide's central region, partially characterized as a -turn, was found exposed form the binding groove. In addition, P1 and P9 residues remained bound in the A and F binding pockets, even though there was a suggestion that P9 was more flexible. The complex lacked numerous water mediated hydrogen bonds that were present in the reference peptide x-ray structure. Moreover, local displacements of residues Asp4, Thr5 and Pro9 resulted in loss of some key interactions with the MHC molecule. This might explain the reduced affinity of the MUC1-9 peptide, relatively to SEV9, for the MHC class I H-2K