11 research outputs found
Trends in the demographic and clinicopathological characteristics in Japanese patients with endometrial cancer, 1990–2010
Postoperative assessment after AVR and TAVI
Background and aims : Severe aortic stenosis (AS) has been normally treated with surgical aortic valve replacement (AVR) whereas recently, transcatheter aortic valve implantation (TAVI) has been introduced as a minimally invasive operation for patients with high surgical risk and frailty. In this study, we have evaluated postoperative physical function and nutrition intake in the patients following AVR and TAVI. Methods : This prospective observational study involved 9 patients with surgical aortic valve replacement (AVR) and 7 patients with transcatheter aortic valve implantation (TAVI). Body composition was measured one day prior surgery, postoperative day (POD) 1, POD 3, POD 5 and POD 7. Hand grip strength, calf circumference and gait speed were measured one day before surgery and on the day of discharge. Results : Skeletal muscle was significantly decreased in AVR patients at postoperative day 3 and 7, while there was no change in TAVI patients. Patients with TAVI showed higher dietary intake after surgery compared to patients with AVR, and they maintained hand grip strength and calf circumference at discharge. Conclusions : In elderly patients with AS, TAVI can improve post-operative recovery maintaining nutritional status and physical function even
Guanine- 5-carboxylcytosine base pairs mimic mismatches during DNA replication
The genetic information encoded in genomes must be faithfully replicated and transmitted to daughter cells. The recent discovery of consecutive DNA conversions by TET family proteins of 5-methylcytosine into 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine (5caC) suggests these modified cytosines act as DNA lesions, which could threaten genome integrity. Here, we have shown that although 5caC pairs with guanine during DNA replication in vitro, G·5caC pairs stimulated DNA polymerase exonuclease activity and were recognized by the mismatch repair (MMR) proteins. Knockdown of thymine DNA glycosylase increased 5caC in genome, affected cell proliferation via MMR, indicating MMR is a novel reader for 5caC. These results suggest the epigenetic modification products of 5caC behave as DNA lesions
Primary retroperitoneal cyst in pregnancy: A case report based on immunohistochemical analysis and literature review
Primary retroperitoneal cystic lesions (PRCLs) are extremely rare, and their histogenesis and clinical evolution remain unclear. Here, we report the case of a 38-years-old primigravida who was referred to our hospital for the delivery. Routine ultrasonographic examination at 36 weeks of gestation revealed the presence of a cystic mass close to the right side of the uterus. One month after the childbirth, we performed the surgery. The cystic mass was present on the right side of the retroperitoneal cavity. We successfully extracted the cyst without rupture. The cyst was 12 × 6 × 3 cm, unilocular, exhibited a thin wall, and contained mucinous liquid. Histological examination revealed that the lining of the cyst comprised two types of adjacent cells. The flat low cuboidal cells were positive for calretinin, cytokeratin (CK) 5/6, CK7, cancer antigen 125 (CA125), and D2-40, all of which are mesothelial markers. The tall columnar cells were also positive for the mesothelial marker CK7. However, the flat low cuboidal cells were negative for mucinous cell makers, while tall columnar cells were positive for these markers. The tall columnar cells contained mucin but were not positive for gastrointestinal epithelial-specific secreted mucin makers. This finding directly supports our hypothesis that the retroperitoneal cysts emerged from the mesothelium
Primary retroperitoneal cyst in pregnancy: A case report based on immunohistochemical analysis and literature review
Expression of p53 in endometrial polyps with special reference to the p53 signature
We herein examined the significance of the
p53 expression in endometrial polyps (EMPs). A total of
133 EMPs, including 62 premenopausal and 71
postmenopausal women with EMP, were immunohistochemically studied for the expression of estrogen
receptor (ER)-alpha, Ki-67 and p53. Apoptotic cells
were identified using a TUNEL assay. A DNA sequence
analysis of TP53 exons 5 to 9 was performed. Among
the premenopausal EMPs, a multivariate analysis
showed the labeling index (LI) for Ki-67 to correlate
significantly with that for p53 (P<0.001), but not that for
apoptosis. On the contrary, among the postmenopausal
EMPs, the LI for Ki-67 correlated significantly with that
for apoptosis (P<0.001). The p53 signature (p53S) was
defined by endometrial epithelial cells, which are
morphologically benign in appearance but display 12 or
more consecutive epithelial cell nuclei with strong p53
immunostaining. The p53S was found in nine (12.7%)
postmenopausal EMPs (mean age: 70.2 years). The
median Ki-67 index for the p53S was 7%, with no
significant difference from that of the glands of the
postmenopausal EMPs without the p53S (P=0.058). The
median apoptotic index for the p53S was 0%, which was
significantly lower than that of the postmenopausal
EMPs without the p53S (P=0.002). Two of four p53Ss
showed TP53 mutations according to the DNA sequence
analysis. The presence of the p53S is not rare in
postmenopausal EMPs with an advanced age. Among
postmenopausal EMPs, the LI of Ki-67 significantly
correlates with that of apoptosis. However, such a
positive correlation between the LI of Ki-67 and
apoptosis is not observed in p53S