14 research outputs found
Bone mechanical properties in healthy and diseased states
The mechanical properties of bone are fundamental to the ability of our skeletons to support movement and to provide protection to our vital organs. As such, deterioration in mechanical behavior with aging and/or diseases such as osteoporosis and diabetes can have profound consequences for individuals’ quality of life. This article reviews current knowledge of the basic mechanical behavior of bone at length scales ranging from hundreds of nanometers to tens of centimeters. We present the basic tenets of bone mechanics and connect them to some of the arcs of research that have brought the field to recent advances. We also discuss cortical bone, trabecular bone, and whole bones, as well as multiple aspects of material behavior, including elasticity, yield, fracture, fatigue, and damage. We describe the roles of bone quantity (e.g., density, porosity) and bone quality (e.g., cross-linking, protein composition), along with several avenues of future research.Author manuscrip
Computational modeling of biological cells and soft tissues
Biological materials are complex hierarchical systems subjected to external
stimuli like mechanical forces, chemical potentials and electrical signals. A deeper
understanding of the behavior of these materials is required for the response
characterization of healthy and diseased conditions. The primary aim of this
dissertation is to study the mechanics of biological materials like cells and tissues from
a computational perspective and relate its behavior with experimental works, so as to
provide a framework for the identification and treatment of pathological conditions like
cancer and vascular diseases.
The first step towards understanding the behavior of a biological cell is to
comprehend its response to external mechanical stimuli. Experimentally derived
material properties of cells have found to vary by orders of magnitude even for the
same cell type. The primary cause of such disparity is attributed to the stimulation
process, and the theoretical models used to interpret the experimental data. The
variations in mechanical properties obtained from the experimental and theoretical studies can be overcome only through the development of a sound mathematical
framework correlating the derived mechanical property with the cellular structure.
Such a formulation accounting for the inhomogeneity of the cytoplasm due to stress
fibers and actin cortex is developed in this work using Mori-Tanaka method of
homogenization. Mechanical modeling of single cells would be extremely useful in
understanding its behavior in an experimental setup.
Characterization of in-vivo response of cells requires mathematical modeling of
the embedding environment like fibers and fluids, which forms the extra cellular matrix.
Studies on fluid-tissue interactions in biomechanics have primarily relied on either an
iterative solution of the individual solid or tissue phases or a sequential solution of the
entire domain using a coupled algorithm. In this dissertation, a new computational
methodology for the analysis of fluid-tissue interaction problem is presented. The
modeling procedure is based on a biphasic representation of fluid and tissue domain,
consisting of fluid and solid phases. The biphasic-fluid interaction model is also
implemented to study the transfer of low-density lipoprotein from the blood to the
arterial wall, and also the nutrient transfer in the tissue scaffolds of a bioreactor
Effects of body size and load carriage on lower-extremity biomechanical responses in healthy women
Abstract
Background
Musculoskeletal injuries, such as stress fractures, are the single most important medical impediment to military readiness in the U.S. Army. While multiple studies have established race- and sex-based risks associated with a stress fracture, the role of certain physical characteristics, such as body size, on stress-fracture risk is less conclusive.
Methods
In this study, we investigated the effects of body size and load carriage on lower-extremity joint mechanics, tibial strain, and tibial stress-fracture risk in women. Using individualized musculoskeletal-finite-element-models of 21 women of short, medium, and tall statures (n = 7 in each group), we computed the joint mechanics and tibial strains while running on a treadmill at 3.0 m/s without and with a load of 11.3 or 22.7 kg. We also estimated the stress-fracture risk using a probabilistic model of bone damage, repair, and adaptation.
Results
Under all load conditions, the peak plantarflexion moment for tall women was higher than those in short women (p < 0.05). However, regardless of the load condition, we did not observe differences in the strains and the stress-fracture risk between the stature groups. When compared to the no-load condition, a 22.7-kg load increased the peak hip extension and flexion moments for all stature groups (p < 0.05). However, when compared to the no-load condition, the 22.7-kg load increased the strains and the stress-fracture risk in short and medium women (p < 0.05), but not in tall women.
Conclusion
These results show that women of different statures adjust their gait mechanisms differently when running with external load. This study can educate the development of new strategies to help reduce the risk of musculoskeletal injuries in women while running with external load
An Integrated Musculoskeletal- Finite-Element Model to Evaluate Effects of Load Carriage on the Tibia During Walking
Prior studies have assessed the effects of load carriage on the tibia. Here, we expand on these studies and investigate the effects of load carriage on joint reaction forces (JRFs) and the resulting spatiotemporal stress/strain distributions in the tibia. Using full-body motion and ground reaction forces from a female subject, we computed joint and muscle forces during walking for four load carriage conditions. We applied these forces as physiological loading conditions in a finite-element (FE) analysis to compute strain and stress. We derived material properties from computed tomography (CT) images of a sex-, age-, and body mass index-matched subject using a mesh morphing and mapping algorithm, and used them within the FE model. Compared to walking with no load, the knee JRFs were the most sensitive to load carriage, increasing by as much as 26.2% when carrying a 30% of body weight (BW) load (ankle: 16.4% and hip: 19.0%). Moreover, our model revealed disproportionate increases in internal JRFs with increases in load carriage, suggesting a coordinated adjustment in the musculature functions in the lower extremity. FE results reflected the complex effects of spatially varying material properties distribution and muscular engagement on tibial biomechanics during walking. We observed high stresses on the anterior crest and the medial surface of the tibia at pushoff, whereas high cumulative stress during one walking cycle was more prominent in the medioposterior aspect of the tibia. Our findings reinforce the need to include: (1) physiologically accurate loading conditions when modeling healthy subjects undergoing short-term exercise training and (2) the duration of stress exposure when evaluating stress-fracture injury risk. As a fundamental step toward understanding the instantaneous effect of external loading, our study presents a means to assess the relationship between load carriage and bone biomechanics
Elastic Anisotropy of Trabecular Bone in the Elderly Human Vertebra
Knowledge of the nature of the elastic symmetry of trabecular bone is fundamental to the study of bone adaptation and failure. Previous studies have classified human vertebral trabecular bone as orthotropic or transversely isotropic but have typically obtained samples from only selected regions of the centrum. In this study, the elastic symmetry of human vertebral trabecular bone was characterized using microfinite element (μFE) analyses performed on 1019 cubic regions of side length equal to 5 mm, obtained via thorough sampling of the centrums of 18 human L1 vertebrae (age = 81.17 ± 7.7 yr; eight males and ten females). An optimization procedure was used to find the closest orthotropic representation of the resulting stiffness tensor for each cube. The orthotropic elastic constants and orientation of the principal elastic axes were then recorded for each cube and were compared to the constants predicted from Cowin's fabric-based constitutive model (Cowin, 1985, “The Relationship Between the Elasticity Tensor and the Fabric Tensor,” Mech. Mater., 4(2), pp. 137–147.) and the orientation of the principal axes of the fabric tensor, respectively. Deviations from orthotropy were quantified by the “orthotropic error” (van Rietbergen et al., 1996, “Direct Mechanics Assessment of Elastic Symmetries and Properties of Trabecular Bone Architecture,” J. Biomech., 29(12), pp. 1653–1657), and deviations from transverse isotropy were determined by statistical comparison of the secondary and tertiary elastic moduli. The orthotropic error was greater than 50% for nearly half of the cubes, and the secondary and tertiary moduli differed from one another (p < 0.0001). Both the orthotropic error and the difference between secondary and tertiary moduli decreased with increasing bone volume fraction (BV/TV; p ≤ 0.007). Considering only the cubes with an orthotropic error less than 50%, only moderate correlations were observed between the fabric-based and the μFE-computed elastic moduli (R(2 )≥ 0.337; p < 0.0001). These results indicate that when using a criterion of 5 mm for a representative volume element (RVE), transverse isotropy or orthotropy cannot be assumed for elderly human vertebral trabecular bone. Particularly at low values of BV/TV, this criterion does not ensure applicability of theories of continuous media. In light of the very sparse and inhomogeneous microstructure found in the specimens analyzed in this study, further work is needed to establish guidelines for selecting a RVE within the aged vertebral centrum
Elastic Anisotropy of Trabecular Bone in the Elderly Human Vertebra
Knowledge of the nature of the elastic symmetry of trabecular bone is fundamental to the study of bone adaptation and failure. Previous studies have classified human vertebral trabecular bone as orthotropic or transversely isotropic but have typically obtained samples from only selected regions of the centrum. In this study, the elastic symmetry of human vertebral trabecular bone was characterized using microfinite element (lFE) analyses performed on 1019 cubic regions of side length equal to 5 mm, obtained via thorough sampling of the centrums of 18 human L1 vertebrae (age ÂĽ 81.17 6 7.7 yr; eight males and ten females). An optimization procedure was used to find the closest orthotropic representation of the resulting stiffness tensor for each cube. The orthotropic elastic constants and orientation of the principal elastic axes were then recorded for each cube and were compared to the constants predicted from Cowin's fabric-based constitutive mode