The Changes of Collagen Synthesis Following the Active Arthus Reaction in the Guinea Pig Skin

Active Arthus reactions were provoked by injecting 100 μg bovine serum albumin (BSA) into the skin of sensitized guinea pigs. Skin biopsy samples were taken 1, 4, 8, 24, 48, 72 and 120 hours after the provocation. Samples were labeled by intradermal injection of 10 μCi [3, 4- 3H]- proline for 1 hour and specific activity of radioactive proline per amount of protein was determined on 1 N NaOH extract of dermis as total protein synthesized. And the synthesized collagenous protein was measured as 3H-hydroxyproline by the method of Juva and Prockop. The results were compared with those obtained by the control injections of 100 μg BSA into the skin of non-sensitized guinea pigs. The synthesized total protein increased at 1, 4 and 8 hours, decreased at 24 hours and increased again at 72 hours. The time course of collagenous protein synthesis was nearly the same as that of total protein synthesis, however the synthesized collagenous protein was higher than the total protein synthesized 1 and 4 hours after the provocation. It was suggested that collagen synthesis increased in an active Arthus reaction up to 4 hours after the provocation

An Attempt to Suppress the Induction of Contact Sensitivity to 2,4-Dinitrochlorobenzene by Tape Stripping Treatment of Guinea Pig Skin

The effect of stripping treatment with cellophane tape on induction of contact sensitivity (CS) to 2,4-dinitrochlorobenzene (DNCB) was studied in inbred JY1 strain guinea pigs in order to determine whether Langerhans cells (LC) are relevant to production of CS. Tape stripping of ear skin achieved, to a considerable degree but not absolutely, depletion of epidermal LC, as measured by cell surface of LC dedetcted by ATPase staining. However, pretreatment of tape stripping on the induction site of contact sensitization with DNCB did not diminish the rate and intensity of challenge reactions to DNCB

Changes in Epidermal Langerhans Cells Following the Tuberculin Reaction in Guinea Pigs

Changes in Langerhans cells (LCs) with the tuberculin reaction were studied using EDTA (ethylenediaminetetraacetic acid) separation and ATPase (adenosine triphosphatase) staining techniques in guinea pig epidermis following intradermal PPD injections. The densities of ATPase positive LCs decreased significantly (p<0.001) from 24 hours to 7 days after injection with a maximum decrease at 72 hours. In addition, these cells became rather swollen or enlarged and lost their characteristic dendritic processes. The significance of these changes is discussed

The Effect of Tape Stripping Treatment of Guinea Pig Skin on the Induction of Delayed Hypersensitivity

The induction of CS to DNCB was impaired if DNP-EC was injected subcutaneously through the skin that was stripped with cellophane tape immediately before the antigen application. The suppressive effect of tape stripping on DNP-EC induced CS was abrogated when normal PM was mixed and injected subcutaneously with DNP-EC into stripped skin. By contrast, tape stripping treatment before BCG or OVA in FIA administration had not influence on development of these delayed hypersensitivity. Significance of these findings is discussed