15 research outputs found
Histochemical Expression of Inducible Nitric Oxide Synthase and Inflammatory Cytokines in Alveolar Macrophages in Patients with ARDS.
A Three-Dimensional Structural Dissection of Drosophila
Abstract. We have analyzed the three-dimensional structural details of Drosophila melanogaster polytene chromosome bands and interbands using three-dimen-sional light microscopy and a novel method of sample preparation that does not involve flattening or stretch-ing the chromosomes. Bands have been visualized in unfixed chromosomes stained with the DNA specific dye 4,6-Diamidino-2-phenylindole (DAPI). Interbands have been visualized using fixed chromosomes that have been immunostained with an antibody to RNA polymerase II. Additionally, these structures have been analyzed using in situ hybridization with probes from specific genetic loci (Notch and white). Bands are seen to be composed of ~36 substructural features that measure 0.2-0.4 txm in diameter. We sug