15 research outputs found

    新生児結腸破裂の一治験例

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    Multiparametric analysis using autostage cytofluorometry.

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    A Three-Dimensional Structural Dissection of Drosophila

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    Abstract. We have analyzed the three-dimensional structural details of Drosophila melanogaster polytene chromosome bands and interbands using three-dimen-sional light microscopy and a novel method of sample preparation that does not involve flattening or stretch-ing the chromosomes. Bands have been visualized in unfixed chromosomes stained with the DNA specific dye 4,6-Diamidino-2-phenylindole (DAPI). Interbands have been visualized using fixed chromosomes that have been immunostained with an antibody to RNA polymerase II. Additionally, these structures have been analyzed using in situ hybridization with probes from specific genetic loci (Notch and white). Bands are seen to be composed of ~36 substructural features that measure 0.2-0.4 txm in diameter. We sug

    From cytofluorometry to fluorescence image analysis.

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