REGIONAL FEATURES OF BIFIDOBACTERIA ANTAGONISTIC ACTIVITY IN INTESTINAL MICROBIOTA OF CHILDREN LIVING IN SIBERIA

Antagonistic properties of bifidobacteria to opportunistic microorganisms in vitro and mechanism of antagonistic activity decreasing were studied and. analyzed. Great percentage of bifidobacteria regional population strains with low antagonistic activity to transitional opportunistic microorganisms is registered. It results to colonization. resistance decreasing and. requires development of the preventive probiotics therapy methods to correct

SPECIES DIVERSITY OF BIFIDOBACTERIA IN CHILDREN WITH FUNCTIONAL DISORDERS OF GASTROINTESTINAL TRACT

The main aim of this research was to identify species ratio and compatibility in children with functional disorders of gastrointestinal tract (GIT) using PCR method, with, species-specific primers for bifidobacteria. The assessment of intestinal microbiota symbionts composition, was made simultaneously. Dysbiosis changes of I—II degree in large intestine microbiocenosis in 86 children examined, were detected. The definition, of bifidobacteria species profile with, the dominance of B. longum, B. catenulatum. and B. bifidum. will allow to optimize the treatment of this pathology

DETECTION OF GENETIC DETERMINANTS OF PATHOGENICITY OF STRAINS OF KLEBSIELLA SPP. ISOLATED FROM THE INTESTINAL BIOTOPE OF CHILDREN WITH FUNCTIONAL GASTROINTESTINAL DISORDERS

Functional gastrointestinal disorders (FGID) are one of the most common problems in children of the first year of life. The aim of the study was to assess the pathogenic potential of Klebsiella spp. strains, isolated from the colon in children of the first year of life with FGID. Material for the study included 61 coprological samples. The biological material was divided into comparison groups, depending on the type of Klebsiella excreted at a concentration of 105–108 CFU/g: 1st – with vegetation in the colon K. pneumoniae (n = 30); 2nd – with vegetation K. oxytoca (n = 31). Bacteriological study composition of the intestinal contents was carried out according to the Industry standard “Protocol of management of patients. Intestinal dysbiosis” (2003). Identification was carried out according to generally accepted schemes using commercial test systems for biochemical identification of bacteria. Statistical data processing was performed using licensed applications “MS Office Excel 2003 for Windows 7”. The data on the quantitative and qualitative changes in the composition microbiota in the comparison groups were obtained. The results of detection genetic determinants of pathogenicity in the samples of Klebsiella of two species show that among the strains of Klebsiella spp., vegetating in the intestines of children as a component of an allochthonous microbiota, a sufficiently high and virulent potential can be concentrated. Detection of pathogenicity genes in bacteria of the genus Klebsiella will expand and deepen the problem of finding the structures of adaptation of strains of bacteria that cause FGID in children of the first year of life

Analysis of phagoand antibiotic sensitivity of Enterobacteriaceae bacteria isolated from women of reproductive age

Pelvic inflammatory diseases occupy a special place in the structure of general morbidity, and are polymicrobial in nature with dominance of opportunistic microorganisms, in particular bacteria of the family Enterobacteriaceae. The aim was to study the composition of the vaginal microbiota in women of reproductive age with pelvic inflammatory diseases, as well as to determine the sensitivity of isolated microorganisms to antibiotics and bacteriophages. The study included 70 women of reproductive age, among them 37 were diagnosed with colpitis and cervicitis, 33 women in the comparison group (women screened for a diagnosis). Isolated microorganisms were identified by abdominoperineal methods, including the disk diffusion method to determine the sensitivity of microorganism cultures of Enterobacteriaceae family to antibiotics, and the method of crosses (evaluation of lytic activity of bacteriophages by the number of crosses) to determine the sensitivity to specific therapeutic bacteriophages. Vaginal biocenosis was characterized by deficit of lactobacilli (< 106 CFU/ml in 100 %), the presence of conditionally pathogenic microflora: bacteria of Enterobacteriaceae family, coccal flora and Candida fungi. From 60.0 to 89.3 % of Enterobacteria strains were resistant to aminoglycosides and quinolones, but also had a low level of sensitivity to therapeutic bacteriophages. The obtained data indicate the reduction of colonization resistance of vaginal mucosa in pelvic inflammatory diseases and specify the need to use medicinal drugs only under medical supervision to prevent clinically significant drug resistance

Detection of genes of pathogenicity of symbiotic microflora in adjacent biotopes in women with chronic endometritis and reproductive disorders

Recent researches established microecological relationships between intestinal, vaginal and nasopharyngeal biocenoses in the composition of a macroorganism microbiome. This study included 57 women with chronic endometritis and 21 woman without it (control group). In women with chronic endometritis all studied pathogenicity genes (asal, cylA, stxl and stx2) were identified in representatives of symbiotic microflora in all three studied habitats - vaginal, intestinal and nasopharyngeal, which confirms the presence of a reservoir of potential pathogenicity. Studied genes of pathogenicity were diagnosed in women with CE in 8 cases of the bacteria of the species E. faecalis and E. faecium, which are "harbingers" of impending serious problem. This indicates the importance of these species in etiopathogenetic structure of microbiocenoses of studied habitats, and, possibly, their impact on the course of this disease

Microecological and associative structure of intestinal biocenosis in children with functional gastrointestinal disorders

The article is devoted to one of actual problems of gastroenterology - study of microbiocenosis of gastrointestinal tract in functional disorders. Functional gastrointestinal disorders (FGID) or minimal dysfunctions of digestion are frequent in the first months of child's life and provoke anxiety both in parents and in pediatricians. Aim: to explore the microbial landscape and the associative structure of the microbiota of the large intestine in children with functional disorders of the gastrointestinal tract. Subjects and methods: Intestinal microbiota composition of 225 children with functional gastrointestinal disorders and 100 healthy children was studied using bacteriological method. Results. The 88.4 % frequency of detection of intestinal eubiosis disorders is discussed. Information is provided on the species composition of the major opportunistic organisms that inhabit this biotope. According to the results, decrease of bifidobacteria amount in structure of large intestine microbiota increases rate of detection of opportunistic microorganisms - predominantly, Klebsiella genus bacteria and Staphylococcus aureus. The intestinal microbial community is dominated by multicomponent transient association. The frequency of detection of pathogenic Staphylococcus in children under one year is discussed in detail. Conclusions. A distinctive feature of the intestinal biocenosis microecological status is significantly greater spread of enterococci than in healthy children, which can be a risk for the emergence of strains with the presence of a number of pathogenecity factors that cause infectious processes

Comparison of microecological intestinal indices in children with different value of body mass index

The body mass index (BMI) is one of the key and at the same time simple and significant indicators of the state of the human body. Both large and small quantities of BMI is associated with high morbidity. The aim of the study was to analyze microecological disorders of intestinal biocenosis in children of different ages using the value of BMI as the criterion of comparison. We researched scatological material from 158 children and adolescents aged from 5 to 17 years (77 boys and 81 girls). The material was divided into three comparison groups depending on the BMI value: group 1 - body mass deficit (n = 34); group 2 - patients having body mass excess (n = 37); group 3 - with normal body mass (n = 87). It has been shown that more than 80 % of children with different BMI value were recorded to have dysbiotic disturbances of the intestinal microbiota of degrees 1 and 2. However, in the group of children with normal body mass, frequency of detection of intestinal dysbiosis was significantly lower; in the group of children with underweight dysbiotic disorders were found significantly more often than in overweight and obese children (p < 0.05). In children with body mass deficit we observed not only increasing concentration of opportunistic pathogenic bacteria, but also an increased occurrence of representative of Enterobacteriаceaе. family. 58.8 % of cases had two- and three-component associations of opportunistic pathogenic bacteria, which is an indication the expressed violations of the intestine microbiocenosis formation. The data obtained show that,you can use BMI, an important criterion reflecting the severity of the disease, in the assessment of intestinal microflora in combination with other studies

DETECTION OF CERTAIN GENETIC MARKERS OF THE PATHOGENIC FACTORS IN AUTOSTRAINS KLEBSIELLA SPP. IN INFANTS

The article presents the results of detection of genetic determinants of pathogenicity in 44 strains of Klebsiella (Klebsiella pneumoniae, Klebsiella oxytoca), isolated from the intestinal habitat of infants with intestinal dysbiosis. The presence of the uge gene was detected in 92 % of cases in K. oxytoca and in 90 % of K. pneumoniae. Kfu gene was detected twice as frequent (30 %) in K. pneumoniae strains than in K. oxytoca (12,5 %); bfp gene was detected in K. oxytoca 5 times more frequently (25 %) than in K. pneumoniae (5 %), as well as stx 1 - 29,2 % and 15 %, correspondingly. The presence of stx 2 gene wasn't recorded in any of the DNA samples. Registration of investigated determinants in DNA of Klebsiella spp. autostrains which are non-clinical isolates indicates pathogenicity factors circulation among them and therefore the risk of the formation of intestinal dysbiosis in children

MICROECOLOGICAL AND GENESPECIFIC CHARACTERISTICS OF VAGINAL BIOTOPE LACTOBACILLI IN WOMEN WITH NONSPECIFIC LOWER FEMALE REPRODUCTIVE TRACT INFLAMMATORY DISEASES

The study included 30 reproductive age women with lower female reproductive tract nonspecific inflammatory diseases. The analysis of the qualitative and quantitative pathogenic microorganisms as well as lactobacilli composition was conducted. It was shown that in women observed the opportunistic microflora detected often (73,3 %) in the rest part of patients (26,7 %) in case if opportunistic microflora was absent the disbiotic changes occurred in lower concentration of normal microflora of vagina represented mostly by lactoflora. Deficiency of lactobacilli was observed almost in all women of this group (96,6 %) and only in one case (3,4 %) the concentration of lactobacilli was consistent with normal physiological range. Molecular genetic methods (PCR amplification) with visualization by means of electrophoresis in agarose gel was made for identification of Lactobacilli species. Consistency index (c) and species saturation index (sri) for opportunistic microorganisms in women vaginal biotope examined were calculated. Prevalence of Lactobacillus plantarum and Lactobacillus jensoni isolating in 83 and 50 % respectively was shown. Also it was shown that in the structure of quantitative compatibility of studied species of lactobacilli the highest rate is characterized to the association of two types that makes 53 %

VAGINAL MICROECOLOGY IN WOMEN WITH THE NON-SPECIFIC GENITAL INFLAMMATORY DISEASES AND REPRODUCTIVE FUNCTION DISORDERS

We conducted the microbiological examination of vaginal microbiota in 324 women with non-specific inflammatory diseases of the genital tract and with reproductive dysfunction. According the condition of microecological balance of indigenous microflora (lactobacilli concentration) of women we have identified three types of vaginal microbiota: "normocenosis", " dysbiosis", "deep dysbiosis" We marked deficiency oflactobacilli in every fourth women (28%) with infertility and miscarriage, and deep deficit in every second (53% in the third group) - sharp depression of the indigenous microbiota and its replacement by opportunistic (facultative anaerobic and obligate anaerobic), what indicates microecological trouble in the vaginal biotope and can be one of the causes of infectious and inflammatory diseases of genitals and other reproductive disorders. It was shown that the dominant microorganisms of pathogenic microbiota (UPM) of inflammatory diseases in women are coagulase-negative staphylococci (CNS), fungi of genus Candida, Escherihia coli and Enterococcus spp. During the analysis of UPM representatives material of groups with "normocenosis", "dysbiosis" and "deep dysbiosis" an important microecological indicator was calculated which characterizing microbiocaenosis as a whole - index of species richness (ISR) - the average number of species in the composition