Photoluminescence Spectroscopy for Estimating the Age of the Latent Fingerprints: A New Potential Approach

This study examined variations in spectral absorption and emission intensity of latent fingerprints over time, employing UV-Vis spectroscopy and photoluminescence spectroscopy. There was noticeable absorption at 296nm on the samples, and the fluorescence emission intensity showed variations.  A total of 1600 samples from various categories were analyzed to validate this hypothesis. The emission intensity observed under photoluminescence spectroscopy varied over time, showing greater intensities in controlled female and male samples compared to the uncontrolled samples that were exposed to environmental conditions. The emission intensity of the controlled female (CF) sample was the highest at 6341168 CPS, followed by the controlled male (CM) sample at 1270240 CPS. On the other hand, the uncontrolled female (UF) sample exhibited an emission intensity of 5431940 CPS, while the uncontrolled male (UM) sample had an intensity of 100764 CPS.  This emission intensity variation proved to be an efficient age profiling marker for the latent fingerprints

Quantifying the Effect of Seasonal Variations on the Latent Fingerprint Residues Through Photoluminescence Spectroscopy

Perspiration fluctuates with the season. Body mass, humidity, temperature, and other factors affect perspiration. Due to higher temperatures, warmer seasons produce more perspiration in the individuals which is the opposite with colder seasons. Seasonal perspiration regulates body temperature in different climates. Due to seasonal and environmental factors, fingerprint residues are subject to variations. Photoluminescence and UV-VIS spectroscopy were used to evaluate fingerprint residues and their response with respect to seasonal variations. To test the hypothesis, 1600 latent fingerprint residues were collected under various conditions spanning the summer and winter seasons cycle for a year. The collected samples were subjected to ultraviolet (UV) light from 200nm to 1600nm to examine their absorption spectra. Further, their fluorescence intensities were measured using photoluminescence spectroscopy. The research's positive outcomes demonstrate that photoluminescence spectroscopy may accurately detect seasonal resonances on latent fingerprint residues, which can be termed seasonal markers

Lighting up Mycobacteria with membrane-targeting peptides.

We report a series of fluorescent probes based on mycobacteria membrane-associated disruption peptide, containing either L- or D-amino acids which were originally designed to kill Mycobacterium tuberculosis via membrane disruption. These peptides were decorated with "always on" and environmentally sensitive fluorophores and showed the rapid and efficient labelling of Mycobacterium smegmatis, with labelling of Mycobacterium tuberculosis demonstrated by two of the probes

Advancement in Age Estimation in Forensic Science Through Molecular Fingerprinting Techniques – A Review Paper

Dactyloscopy has long been used for personal identification from the latent fingerprint residues, capable of providing an insight into various factors of an individual, such as age, sex, habits and lifestyle. Various studies have been conducted to precisely identify the changes in activity of sweat glands and the chemical breakdown of fingerprint residues with respect to time. However, a reliable method for accurately estimating or approximating the age of the fingerprint donor is yet to be established. The emerging field of molecular fingerprinting analyses latent fingerprint sweat residue and profiles the components present in it, which aids in personal identification as an individualistic marker specific to each individual. This review article highlights the advancements in estimating the age of the fingerprint donor from latent fingerprint residue and addresses the technical and technological research gaps in the timeline of molecular fingerprinting techniques, as this method holds potential in aiding forensic investigation and criminal profiling from the fingerprints retrieved from the scene of crime

Magnetic hydroxyapatite nanomaterial–cyclodextrin tethered polymer hybrids as anticancer drug carriers

Osteosarcoma, the most common bone cancer, leads to a poor survival rate of patients. Drug targeting employing hydroxyapatite (HAp)-based nanocarriers represents a fascinating choice for non-invasive treatment of osteosarcoma. Herein, we report strontium-doped (Sr-HAp) and iron- and strontium-co-doped (Sr,Fe-HAp) hydroxyapatite nanoparticles as novel materials that deliver doxorubicin to bone cancer cells. A platinum-complexed and cyclodextrin-functionalized chitosan derivative is utilized to coat the NPs. Sr-HAp (aspect ratio ∼20) and Sr,Fe-HAp (aspect ratio ∼3) nanoparticles are formed as nanowhiskers and nanorods, respectively, as revealed by transmission electron microscopy. Strontium ferrite NPs are synthesized and their properties are compared with those of the Sr/Sr,Fe-doped HAp NPs. These ferrite NPs show ferromagnetic behavior, as opposed to Sr-HAp and Sr,Fe-HAp. The latter two respectively display paramagnetic and superparamagnetic behaviors. The loading percentage of the anticancer drug, Doxorubicin (Dox), in the nanocarriers is high and the release of Dox is sustained at physiological pH. The Dox-loaded nanocarriers are tested for their in vitro cytotoxicity against lung, cervical, liver, and bone cancer cell lines. In general, the efficacy of Dox is not diminished on loading in the nanocarriers. In addition, the Dox-carriers demonstrate a time- and dose-dependent cytotoxicity. The efficacy is enhanced in the case of Dox-loaded carriers on MG-63 (osteosarcoma) cell lines. The anticancer activity is tested in vivo on both male and female albino mice. Enhanced chemotherapeutic potential is observed for Dox-loaded Sr,Fe-HAp in a metastatic model of MG-63. The platinum derivative polymer possesses its own therapeutic effect and contributes to the general activity. The novel polymer-HAp nanohybrid represents an effective nanocarrier for the treatment of osteosarcoma

Magnetic hydroxyapatite nanomaterial–cyclodextrin tethered polymer hybrids as anticancer drug carriers

Osteosarcoma, the most common bone cancer, leads to a poor survival rate of patients. Drug targeting employing hydroxyapatite (HAp)-based nanocarriers represents a fascinating choice for non-invasive treatment of osteosarcoma.</p