Heterodera glycines Infection Increases Incidence and Severity of Brown Stem Rot in Both Resistant and Susceptible Soybean

Growth chamber experiments were conducted to investigate whether parasitism by Heterodera glycines, the soybean cyst nematode, increases incidence and severity of brown stem rot (BSR) of soybean, caused by Phialophora gregata, in both resistant and susceptible soybean cultivars. Soybean genotypes with various combinations of resistance and susceptibility to both pathogens were inoculated with P. gregata alone or P. gregataplus H. glycines. In most tests of H. glycines-susceptible genotypes, incidence and severity of internal stem discoloration, characteristic of BSR, was greater in the presence than in the absence of H. glycines, regardless of susceptibility or resistance to BSR. There was less of an increasing effect of H. glycines on stem symptoms in genotypes resistant to both BSR and H. glycines; however, P. gregata colonization of these genotypes was increased. Stems of both a BSR-resistant and a BSR-susceptible genotype were colonized earlier by P. gregata in the presence than in the absence of H. glycines. Our findings indicate that H. glycines can increase the incidence and severity of BSR in soybean regardless of resistance or susceptibility to either pathogen

Coupling Soybean Cyst Nematode Damage to CROPGRO-Soybean

The soybean cyst nematode (SCN) Heterodera glycines Ichinohe is responsible for substantial economic losses in soybean (Glycine max L. Merr.) production throughout the U.S. Results from past efforts to quantify the severity of crop damage resulting from SCN are often subject to variable experimental conditions resulting from differences in weather, soil type, and cultivar. Because of the difficulty in accounting for these variables, a process–oriented crop growth simulation model was chosen as a platform for studying the dynamics of SCN damage and for transferring knowledge between crop production scenarios. The objective of this study was to develop and evaluate hypotheses for coupling SCN damage to the process–oriented crop growth model CROPGRO–Soybean. A monomolecular function was used to relate daily SCN damage to initial population density of SCN eggs. The equation was incorporated into the crop model in order to test two hypotheses of how SCN damage occurs. The first hypothesis was that SCN reduce daily photosynthesis (Pg), while the second hypothesis was that SCN reduce daily potential root water uptake (RWU). Canopy biomass data collected in 1997 and 1998 from a site in Iowa were used to estimate damage function parameters for two distinct coupling points, one applied to reduce daily photosynthesis (Pg) and the other applied to reduce daily potential root water uptake (RWU). Function parameters were estimated by minimizing the log transformation of root mean square error (RMSE) between predicted and measured canopy biomass collected every 2 weeks during the season in Iowa. Biomass data collected in 1997 and 1998 from an independent site in Missouri were used to validate the SCN damage models. The minimum root mean squared errors (RMSE) of canopy and grain biomass were 0.245 and 0.198 log10(kg ha–1), respectively, for the RWU coupling point, and 0.238 and 0.193 log10(kg ha–1), respectively, for the Pg coupling point at the independent site in Missouri. The damage functions transferred very well to the independent site. Validation showed that the Pg coupling point represented the variability of both canopy and final yield data slightly better than the RWU coupling point

A New Greenhouse Method to Assay Soybean Resistance to Brown Stem Rot

Greenhouse, growth chamber, and field experiments were conducted to develop a method to assess resistance of soybeans to Cadophora gregata (Phialophora gregata), causal agent of brown stem rot (BSR). In the new method, C. gregata is introduced at the base of the stems of 2-week-old soybeans, and the presence of the fungus is assessed in the tips of the stems 5 weeks later. To test the effectiveness of the method, two populations of soybeans and 10 checks were inoculated at the stem base and then assayed for fungal colonization of the stem tips, percentage of symptomatic leaflets, and percent internal stem length discolored. The lines also were planted in naturally infested fields to assess for percent internal stem length discolored, and were tested for the presence/absence of a BSR-resistant molecular marker. Greenhouse, field, and molecular marker data were compared. Linear regression analysis suggested that percentage of plants with colonized stem tips explained 41 to 64% of the variability (P \u3c 0.0001) in percent stem length discolored in the field and 58 to 85% of the variability (P \u3c 0.0001) in molecular marker data for BSR resistance. Percent stem length discolored assessed in the greenhouse had the lowest correlation with percent stem length discolored in the field and with the molecular marker. Of three incubation temperatures tested, 22°C was the most conducive for distinguishing resistant/susceptible soybeans using the colonization method

Regional Assessment of Soybean Brown Stem Rot, Phytophthora sojae, and Heterodera glycines Using Area-Frame Sampling: Prevalence and Effects of Tillage

The prevalence of brown stem rot (caused by Phialophora gregata), Heterodera glycines, and Phytophthora sojae in the north central United States was investigated during the fall of 1995 and 1996. Soybean fields were randomly selected using an area-frame sampling design in collaboration with the National Agricultural Statistics Service. Soil and soybean stem samples, along with tillage information, were collected from 1,462 fields in Illinois, Iowa, Minnesota, Missouri, and Ohio. An additional 275 soil samples collected from Indiana were assessed for H. glycines. For each field, the incidence and prevalence of brown stem rot was assessed in 20 soybean stem pieces. The prevalence and recovery (expressed as the percentage of leaf disks colonized) of P. sojae and the prevalence and population densities of H. glycines were determined from the soil samples. The prevalence of brown stem rot ranged from 28% in Missouri to 73% in Illinois; 68 and 72% of the fields in Minnesota and Iowa, respectively, showed symptomatic samples. The incidence of brown stem rot was greater in conservation-till than in conventional-till fields in all states except Minnesota, which had few no-till fields. P. sojae was detected in two-thirds of the soybean fields in Ohio and Minnesota, whereas 63, 55, and 41% of the fields in Iowa, Missouri, and Illinois, respectively, were infested with the pathogen. The recovery rates of P. sojae were significantly greater (P ≤ 0.05) in conservation-till than in conventional-till fields in all states except Iowa. H. glycines was detected in 83% of the soybean fields in Illinois, 74% in Iowa, 71% in Missouri, 60% in Ohio, 54% in Minnesota, and 47% in Indiana. Both the prevalence and population densities of H. glycines were consistently greater in tilled than in no-till fields in all states for which tillage information was available

Brown Stem Rot and its Interaction with the Soybean Cyst Nematode

Brown stem rot (BSR) of soybeans is caused by the fungal vascular pathogen Cadophora gregata (previously named Phialophora gregata). BSR is an economically important disease of soybeans in the north central United States, being prevalent in 68 to 73% of the soybean fields of Illinois, Iowa, and Minnesota (Workneh et al. 1999). There are two genetic types (called genotypes) of C. gregata that differ in their ability to cause foliar symptoms on susceptible soybeans (Chen et al. 2000). Infection by genotype A of the fungus can result in mild to severe brown discoloration of the pith and severe foliar symptoms on susceptible soybeans and mild or no foliar symptom on resistant soybeans. In contrast, infection by genotype B of the fungus causes mild to severe brown discoloration of the pith, but mild or no foliar symptoms. Soybeans can be colonized by both genotypes of the fungus without exhibiting stem or foliar symptoms (Taboret al. 2003a). Consequently, hidden yield loss due to BSR may frequently occur

Rising Conservation Tillage Acreage: Implications for Phytophthora Root and Stem Rot of Soybean

Cropping practices are shifting to conservation tillage to counter the growing threat of wind and water erosion due to excessive soil disturbances by conventional tillage practices. In addition, reduction in costs of production and the availability of effective herbicides and drill-planters have played a significant part in decision making. Conservation tillage is defined as any tillage systems that maintain at least 30% of the soil surface covered by residues after planting (National Resource Conservation Service). No-till, mulch-till and ridge-till tillage systems meet the residue level requirement, and their practice is currently on the rise both at the state and national level