Some Issues Regarding the New Rural Development Program in Vietnam

The research focuses on presenting and analyzing several key concepts related to the New Rural Development Program in Vietnam. The presentation begins by defining crucial terms such as rural and new rural in Vietnam. Additionally, the article outlines the legal foundations and the government's target programs for building new rural areas, while discussing the necessity of constructing new rural areas in the current developmental context. This is a detailed and comprehensive article, offering readers an overview of the history and content of this program in enhancing the quality of life in rural Vietnam

Optimization of L-asparaginase production from Escherichia coli using response surface methodology

Among the antitumor drugs, bacterial enzyme L-asparaginase has been employed as the most effective chemotherapeutic agent in pediatric oncotherapy especially for acute lymphoblastic leukemia. In previous study, the L-asparaginase from Erwinia chrysanthermy was expressed in Escherichia coli BL21(DE3). The recombinant L-asparaginase was produced from recombinant E.coli BL21(DE3) under different cultivation conditions (inducer concentration, inoculum concentration and KH2PO4 concentration). The optimized conditions by response surface methodology using face centered central composite design. The analysis of variance coupled with larger value of R2 (0.9) showed that the quadratic model used for the prediction was highly significant (p 0.05). Under the optimized conditions, the model produced L-asparaginase activity of 123.74 U/ml at 1.03 mM IPTG, 3% (v/v) inoculum and 0.5% (w/v) KH2PO4. Recombinant protein was purified by two step using gel filtration and DEAE chromatography. The purified L-asparaginase had a molecular mass of 37 kDa with specific activity of 462 U/mg and identified by MALDI-TOF mass spectrometry. Results of MALDI-TOF analysis confirmed that recombinant protein was L-asparaginase II. Recombinant L-asparaginase has antiproliferative activity with K562 cell line. In conclusion, this study has innovatively developed cultivation conditions for better production of recombinant L-asparaginase in shake flask culture

Mutagenesis development of actinoplanes sp. KCTC 9161 by N-methyl-N'-nitro-N-nitrosoguanidine (NTG) and screening for acarbose production

Acarbose has been widely used in the therapy of type II diabetes (non-insulin dependent) because it controls blood sugar contents of patients after meals. Acarbose, a pseudo-oligosaccharide, acts as a competitive -glucosidase inhibitor. Acarbose is produced by the strains of Bacillus, Streptomyces and Actinoplanes sp. The aim of this study was to develop mutagenesis for an Actinoplanes sp. strain and screening for acarbose production. The spores of Actinoplanes sp. KCTC 9161 strain were subjected to be mutated by N-methyl-N'-nitro-N-nitrosoguanidine (NTG) for screening and finding mutant strains that were capable of production of higher acarbose (an inhibitor of α-glucosidase) higher than wild type strain. Firstly, the original NTG solution was prepared in phosphate buffer 0.05 M, pH 6.9 and the safety concentration of NTG was determined at 5 mg/ml. Then, the spores were incubated with different NTG amounts and duration. The living colonies were transferred to fermentation medium. The results obtained showed that 15 mutant strains were produced higher acarbose than wild type when used thin layer chromatography method for analysis and comparing with standard acarbose (Sigma). Three cell lines among total tested 15 mutant lines of Actinoplanes sp. KCTC 9161 produced acarbose at a higher level or indicated a higher inhibitory activity toward α-glucosidase than the original strain. Enzymatic inhibitory ativity of α-glucosidase of three mutant strains (Actinoplanes sp. KCTC- L4, L11, L14) was increased 1.3 fold higher than wild type and Actinoplanes sp. KCTC spores were very sensitive to NTG toxic, 98% spores could not survive at the treatment condition of 50 µg NTG for 30 minutes. In addition, an applicable protocol for mutating Actinoplanes sp. using NTG was suggested for further research

Determination of the Administration Routes, Doses and Appropriate Age to Vaccinate With Ornitin Triple Vaccine For Cross-breed Colored Broilers in Vietnam

The study was to determine the appropriate dose and administration route of Ornitin Triple vaccine in cross-breed coloured broilers in Vietnam by evaluating the antibody titer against Ornithobacterium rhinotracheale (ORT) and local reactions at injection sites on chickens after vaccination. The study was divided into 2 trials. Both trials were designed with 3 vaccine dose groups: 0.0ml (control group), 0.25ml and 0.5ml and 2 different administration routes: subcutaneous at neck (SC) and intramuscular at breast (IM) injection. The result showed that, no statistically significant difference was found between antibody titer of two administration routes as well as 2 vaccine doses until 13-week-old. Local reactions at the injection sites of IM route was less severe than SC at neck and in higher dose would produce a more severe swelling reaction. Daily weight gain was found to have a slight decrease in the vaccinated groups within 2 weeks after vaccination, however, no statistically significant difference was found in later stage (P > 0.05). In conclusion, Ornitin Triple can be used to vaccinate by IM with the dose of 0.25ml for coloured broilers at early age (3-week-old), or 0.5ml for older birds and should be careful for some reactions at the injection sites

ESP Students’ Perceptions of the Role of Community Language Learning on Learning Motivation: A Case at a College of Medicine and Pharmacy in the Mekong Delta

Community language learning (CLL) is a language teaching method in which students work together to develop what aspects of a language they would like to learn. It is based on the Counselling-approach in which the teacher acts as a counselor and the learner as a client. CLL techniques are believed to help learners alleviate anxiety, threat and the personal and language problems a person encounters in using the language. The research entitled: “An Investigation into ESP Students’ Perceptions of the Role of Community Language Learning on Learning Motivation: A study in a college of medicine and pharmacy in the Mekong Delta” was conducted to find out ESP students’ perception of the role of CLL on their learning motivation. It was conducted with the participation of 144 ESP students from a College in Can Tho city using the questionnaire. The result shows that ESP students’ perception of the role of CLL on their learning motivation was quite high although the level of perceptions on their interest was not high

Tannins: Extraction from Plants

The chapter presents mainly on different extraction methods of tannin. Some technical means required for effective extraction are also presented, for example, collection and treatment of plant and drying and storage of plant. Opportunity and challenges in application of extraction methods are also exhibited in the chapter

Cytotoxic naphthoquinones from Diospyros fleuryana leaves

In the search for anti-cancer plants in Vietnam, the leaves of Diospyros fleuryana were selected for chemical investigation. Phytochemical analysis of the ethyl acetate (EtOAc) extract led to the isolation of two naphthoquinones isodiospyrin (1), and 8'-hydroxyisodiospyrin (2), and one isoflavone 7-O-methylbiochanin A (3). The chemical structures of isolated compounds were determined by 1D-NMR (1H, and 13C-NMR), 2D-NMR spectra (HSQC, and HMBC), and MS spectroscopy. Compound 3 was isolated from genus Diospyros for the first time. Regarding the strong IC50 values of 2.27, and 8.0 µM against KB, and Hep cell lines respectively, cytotoxic examination suggested that compound 2 is a promising agent in anti-cancer treatment.Â

Child stunting is associated with child, maternal, and environmental factors in Vietnam

Child stunting in Vietnam has reduced substantially since the turn of the century but has remained relatively high for several years. We analysed data on children 6–59 months (n = 85,932) from the Vietnam Nutritional Surveillance System, a nationally representative cross‐sectional survey. Multivariable Poisson regression models were used to estimate relative risk (RR) of stunting, stratified by child age and ecological region. Covariates at the child, maternal, household, and environmental levels were included based on available data and the World Health Organization conceptual framework on child stunting. Among children 6–23 months, the strongest associations with child stunting were child age in years (RR: 2.49; 95% CI [2.26, 2.73]), maternal height < 145 cm compared with ≥150 cm (RR: 2.04; 95% CI [1.85, 2.26]), living in the Northeast compared with the Southeast (RR: 2.01; 95% CI [1.69, 2.39]), no maternal education compared with a graduate education (RR: 1.77; 95% CI, [1.44, 2.16]), and birthweight < 2,500 g (RR: 1.75; 95% CI [1.55, 1.98]). For children 24–59 months, the strongest associations with child stunting were no maternal education compared with a graduate education (RR: 2.07; 95% CI [1.79, 2.40]), living in the Northeast compared with the Southeast (RR: 1.94; 95% CI [1.74, 2.16]), and maternal height < 145 cm compared with ≥150 cm (RR: 1.81; 95% CI [1.69, 1.94]). Targeted approaches that address the strongest stunting determinants among vulnerable populations are needed and discussed. Multifaceted approaches outside the health sector are also needed to reduce inequalities in socioeconomic status.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/151838/1/mcn12826.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/151838/2/mcn12826_am.pd

Using metagenomics tool to evaluate the enrichment efficiency of methanogens in marine sediment in Truong Sa archipelage, Khanh Hoa province, Vietnam

Methanogens (archaea bacteria that produce methan) play an important role in the anaerobic digestion of organic waste, however, in marine environment, the low density of methanogens makes anaerobic digestion very slow. In this study, we used methagenomics tool to evaluate the enrichment efficiency of methanogens communities in marine sediments as a basis for building a anaerobic microbial formulation to treat organic waste in marine environment. The results of determination of methanogen density by MPN method have shown that methanogen has been accumulated with higher density (up to 3.2.107 MNP/ml) through 2 times of enrichment in artificial seawater with CH3COONa 10 mM substrate. In addition, metagenomics data have also shown a decrease in the number of archaea species through each enrichment, indicating that methanogenic species have gradually dominated the microbial community. This is the first study on using metagnenomics tools to evaluate the enrichment of methanogens in marine sediment samples in Truong Sa archipelago, Vietnam. Metagenomics data help provide more reliable evidence in microbial studies, especially in obligate anaerobes such as methanogens

ĐẶC ĐIỂM TƯỚNG ĐÁ - CỔ ĐỊA LÝ PLEISTOCEN MUỘN-HOLOCEN KHU VỰC CỬA SÔNG BA LẠT

Lithofacies and paleogeographical characteristics of late Pleistocene-Holocene deposits are related to three sedimentary system tracts: Lowstand systems tract (LST) corresponds to the low regressive phase from 50 ka BP to 18 ka BP. The sedimentary accumulative space is situated from the boundary of weathering crust to the 100 m water depth. In Ba Lat rivermouth area, the lowstand systems tract is dominated by the alluvial silty sand facies group (arLST); Transgressive systems tract (TST) in Ba Lat rivermouth area constitutes a lithofacies section including three facies in upward direction as follows: (1) Transgressive alluvial silty - sand facies (atTST). (2) Transgressive estuary sandy - mud facies (amtTST). (3) Lagoonal maximum transgressive greenish clay facies (mtTST); Highstand systems tract (HST) in the downstream Red river delta area constitutes a deltaic plain structure consisting of three parts: High subaerial delta, low subaerial delta and subaqueous delta, which had been formed from 5 ka BP.Đặc điểm tuớng đá - cổ địa lý trầm tích Pleistocen muộn-Holocen khu vực cửa sông Ba Lạt gắn liền với ba miền hệ thống trầm tích: Miền hệ thống trầm tích biển thấp (LST) tương ứng với pha biển thoái thấp từ 50.000 năm BP đến 18.000 năm cách ngày nay. Không gian tích tụ của miền hệ thống này được giới hạn từ ranh giới miền xâm thực (vỏ phong hóa) đến độ sâu 100 m nước. Trong khu vực cửa Ba Lạt nhóm tướng aluvi biển thoái thống trị (arLST); Miền hệ thống trầm tích biển tiến (TST) trong khu vực cửa sông Ba Lạt cấu thành một mặt cắt gồm ba tướng từ dưới lên: (1) tướng cát bột aluvi biển tiến (atTST), (2) tướng bùn cát cửa sông biển tiến (amtTST) và (3) tướng sét xám xanh vũng vịnh biển tiến cực đại (mtTST); Miền hệ thống trầm tích biển cao (HST) trong khu vực hạ lưu châu thổ sông Hồng tạo nên một cấu trúc đồng bằng châu thổ bao gồm: Đồng bằng châu thổ cao, Đồng bằng châu thổ thấp và châu thổ ngập nước có tuổi từ 5.000 năm BP