1 research outputs found
Analysis of pRb, p16INK4A proteins and proliferating antigens, PCNA, Ki-67 and MCM5 expression in aggressive fibromatosis (desmoid tumor)
Aggressive fibromatosis (desmoid tumor) is
a mesenchymal lesion originating from fascial,
aponeurotic and muscular connective tissue. It rarely
becomes histologically malignant. In this study we
analyzed the cell cycle regulation proteins: pRb, p16,
and proliferating antigens: Ki-67, PCNA, MCM5 with
immunohistochemical method in archival material
derived from 27 extra-abdominal (E-AD), 18 abdominal
(AD) and 5 intra-abdominal (I-AD) cases of desmoid
tumor.
None of the examined cases (n=50) of aggressive
fibromatosis was pRb-immunonegative. Heterogeneous
expression of pRb was observed in 51.85% (14/27) of
Group AD cases and in 5.56% (1/18) of Group E-AD
cases; positive expression in 48,15% (13/27) of Group
AD cases, in 94.44% (17/18) of Group E-AD cases, and
in 100% (5/5) of Group I-AD cases. There were no
negative cases for p16 staining in any of the examined
groups. The number of heterogeneous cases in individual
groups was: 33.33% (9/27) in Group AD, 50% (9/18) in
Group E-AD and 40% (2/5) in Group I-AD, and positive
cases: 66.67% (18/27), 50% (9/18) and 60% (3/5),
respectively. Overexpression of PCNA was noted in
98% (49/50) of cases. The positive staining for Ki-67
protein was noted in 25.93% (7/27) in Group AD, in
16.67% (3/18) in Group E-AD and in 60% (3/5) in
Group I-AD. None of the examined cases was
immunopositive for MCM5 protein.
The noted levels of pRb and p16 expression in
desmoid cells reflect their function in cell cycle regulation. Probably the unsettled cell cycle progression,
especially in G1 phase, is not the cause of aggressive
fibromatosis pathogenesis