Epidemic hepatitis E: serological evidence for lack of intrafamilial spread

To understand the dynamics of intrafamilial spread of the hepatitis E virus a study was conducted using blood samples collected during the 1988 and 1989 epidemics of viral hepatitis in Kudal and Atit villages of Maharashtra state, India; the epidemics were subsequently shown to be due to hepatitis E virus (HEV). The one-time collection carried out at the end of the Kudal epidemic was from 184 apparently healthy individuals irrespective of family history of jaundice during the epidemic. In the Atit epidemic, 153 family contacts of 49 IgM anti-HEV positive patients were bled. An additional 151 blood samples were collected from apparently healthy individuals irrespective of family history of jaundice during the epidemic. One month later, blood samples were collected from 64 of the 153 family contacts. Relevant history was recorded each time. All serum samples were tested for ALT levels and for IgM and IgG antibodies to hepatitis E virus employing ELISA. IgM anti-HEV positivity among persons with family history of jaundice was not different from those without such a history (8/62 (12.9%) and 11/122 (9%) at Kudal; 9/57 (15.8%) and 22/94 (23.4%) at Atit; P > 0.1). Excluding IgG anti-HEV positive samples from the analysis also yielded non-significant results. Of the 32 follow-up samples collected from family contacts without IgG or IgM antibodies to HEV in the initial blood sample, 31 remained IgM and IgG anti-HEV negative at the end of 1 month. One of the family contacts was found to be IgG anti-HEV positive in the second blood sample. The disease was not related to the index case. Intrafamilial spread of HEV is negligible

Exposure of STD patients and prisoners from Pune to hepatitis B and C viruses

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Insignificant prevalence of antibodies to hepatitis C in a rural area of Western Maharashtra

Objective: To determine the age-specific seroprevalence of hepatitis C virus (HCV) in a rural population in Maharashtra. Methods: 1054 serum samples collected from apparently healthy persons were tested by recombinant immunoblot assay for antibodies against HCV (anti-HCV). Anti-HCV positive samples were tested for HCV-RNA by nested reverse transcriptase polymerase chain reaction (RT-PCR). Results: One man tested positive for anti-HCV; his sample was also HCV-RNA positive. Conclusions: HCV infection is infrequent in this rural area in Maharashtra