Genetic analyses of some central anatolian domestic duck populations with inter simple sequence repeat (ISSR): A preliminary study

The aim of this study is to investigate the genetic structure of some domestic duck populations from Kirsehir and Yozgat provinces of the Central Anatolia region of Turkey. Blood samples were obtained from the venae cutenea ulnaris of 76 ducks from four different locations. Eleven ISSR primers produced 73 reproducible and bright bands. The number of polymorphic loci was 72 and the percentage of polymorphic loci was 98.6%. Gene diversity (HT) in total population and magnitude of differentiation among populations (GST) were 0.198 and 0.183, respectively. The genetic distances between regions under investigation were found among 0.0157 and 0.0991. Analysis of molecular variance (AMOVA) reflected that 83% of within variation and 17% among population variation. First three given values of principle coordinate analysis explained 69.8% of total variation as 45.6, 13.8 and 10.4%, respectively. The dendrogram showed two main branches: one contains Kirsehir (L1, L2, L3), the other includes Yozgat region (L4). Shannon's Information index (I) value was 0. 331. The gene flow (Nm) among populations was analyzed and Nm value was estimated as 2.23 with the low level of differentiation among populations. The results indicated that genetic variations of some Central Anatolian domestic duck populations are determined using ISSR marker and might provide information for future breeding strategies. Copyright 2015 Zoological Society of Pakistan

Genetic variation in honey bee (Apis mellifera L.) populations from Bulgaria [Zmiennosc Genetyczna w populacjach pszcoly miodnj(Apis mellifera L.)w Bulagartoii]

Genetic variation of honey bee populations from 9 different locations of Bulgaria was studied using 4 enzymic systems (MDH, ME, EST and ALP) corresponding to 4 genetic loci and PCR- RFLP's analysis of 16s rDNA, COI, and ND5 gene segments of mtDNA. Allozyme analysis revealed that all loci were polymorphic in almost all studied populations. The observed heterozygosity was found to range from 0.146 to 0.258, and Nei's genetic distance between 0.006 and 0.057 among the populations. Bulgarian honey bees are clustered into three groups in neighbor-joining and UPGMA dendrograms. The Pomorie and Slivovik populations are in a separate clade while all other populations are grouped together. The mtDNA analyses revealed no variation in the Bulgarian honey bees. A comparison was made of the results of similar analyses on honey bees from the neighboring countries of Greece and Turkey. The results showed that honey bee populations from Bulgaria and Greece considered to belong to A. m. macedonica subspecies are discriminating, which means the bees show differences

The effect of different feeding strategies on honey bee gut microbiota and the presence of Nosema

Colony development in honey bees depends on both the environmental conditions and their genetic structure. Nutrition is the one of the most important factors in the honey bee’s health. This study was carried out in 48 colonies and included six groups (a control and five feeding groups). In the experiment, the effect of different feeding strategies on intestinal flora and the presence of Nosema was investigated. Pantoea agglomerans, Pseudomonas luteola, Burkholderia cepacia, Brevibacillus nitrificans, Sphingomonas paucimobilis, Aeromonas hydrophila, and P. alcalidimonas were detected in the intestinal microflora of the bee samples by morphological and phenotypic identification. The results of phenotypic identification were confirmed using 16S rRNA sequence analysis for P. agglomerans and B. nitrificans strains. The presence of Nosema was simultaneously investigated in all groups. Only Nosema cerenae was detected using DNA analysis in the positive Nosema spore samples. © 2022, South African Journal for Animal Sciences. All rights reserved.This article was produced by the project “The Pollen Preferences of Honeybees and the Effects of Pollen Use in Winter on Colony Dynamics” (TAGEM/HAYSÜD/B/20/A4/P5/1890), supported by The Turkish Ministry of Agriculture and Forestry, General Directorate of Agricultural Research and Policies (TAGEM). We would like to acknowledge TAGEM and the Aegean Agricultural Research Institute for their support.ÜD/B/20/A4/P5/1890; Agricultural Research Institute, ΙΓ