4 research outputs found
Liquid-liquid equilibrium and partitioning features of bovine trypsin in Ucon 50 HB5100 /sodium citrate aqueous two phase systems
The phase diagrams of Ucon 50-HB-5100, a non-ionic random copolymer of ethylene oxide and propylene
oxide (EOPO) and sodium citrate aqueous two-phase systems were determined at different pHs (5.20
and 8.20) and temperatures (5, 20 and 40潞 C). The binodal curves were determined by refractive index and
enzymatic assay of the solution and described using a four-parameter sigmoidal equation, the reliability
of the measured tie line compositions was ascertained by correlation equations given by Othmer Tobias
and Bancroft. The two-phase area was expanded by increasing both pH and temperature. The partitioning
of bovine trypsin and a-chymotrypsin, proteases of similar physico-chemical properties was investigated
in order to evaluate the applicability of partitioning as a putative method to isolate from pancreas and to
obtain any information about their partitioning mechanism. The effect of different factors such as pH, tie
line length and the presence of an inorganic salt on the protein partition coefficient were analyzed.Se caracterizaron las curvas binomiales correspondientes a los sistemas bif谩sicos acuosos formados por
Ucon 50-HB-5100, un copol铆mero al azar de 贸xido de etileno y 贸xido de propileno (EOPO) y citrato de
sodio a diferentes pHs (5,20 y 8,20) y temperaturas (5, 20 y 40潞 C). Las curvas binomiales se obtuvieron
por determinaci贸n del 铆ndice de refracci贸n y ensayos enzim谩ticos de las soluciones correspondientes; las
composiciones de las l铆neas de uni贸n se corroboraron por las ecuaciones propuestas por Othmer Tobias y
Bancroft. El aumento del pH y la temperatura condujeron a un aumento del 谩rea bif谩sica. Tambi茅n se
ensay贸 el comportamiento de reparto de dos proteasas tripsina y 伪-quimotripsina con el objetivo de
emplear los principios de partici贸n como m茅todo de aislamiento y purificaci贸n de Tripsina a partir de p谩ncreas
bovino y de obtener informaci贸n acerca del mecanismo de partici贸n de la misma. Se analiz贸 el efecto
del pH, longitud de la l铆nea de uni贸n y presencia de sales inorg谩nicas sobre el coeficiente de partici贸n
de las enzimas
Characterization of liquid-liquid equilibria of aqueous two-phase systems containing a termosensible polymer (UCON) and sodium citrate
CONICETALFA Valnatura - Programme European Commissio