Treatment Strategy for Pancreatic Neuroendocrine Neoplasms (pNEN)

Article信州医学雑誌 68(5): 235-242(2020)journal articl

IL-15-High-Responder Developing NK Cells Bearing Ly49 Receptors in IL-15(-/-) Mice

信州大学博士（医学）・学位論文・平成24年3月31日授与（甲第910号）・好沢克Originally published in The Journal of Immunology. Yoshizawa, K; Nakajima, S; Notake, T; Miyagawa, SI; Hida, S; Taki, S. 2011. IL-15-High-Responder Developing NK Cells Bearing Ly49 Receptors in IL-15(-/-) Mice . J. Immunol. Vol.187(10): pp5162-pp5169. Copyright (C) 2011 The American Association of Immunologists, Inc.In mice lacking IL-15, NK cell development is arrested at immature stages, providing an opportunity to investigate the earliest developing NK cells that would respond to IL-15. We show in this study that immature NK cells were present in the spleen as well as bone marrow (BM) and contained IL-15-high-responder cells. Thus, mature NK cells were generated more efficiently from IL-15(-/-) than from control donor cells in radiation BM chimeras, and the rate of IL-15-induced cell division in vitro was higher in NK cells in the spleen and BM from IL-5(-/-) mice than in those from wild-type mice. Phenotypically, NK cells developed in IL-15(-/-) mice up to the minor but discrete CD11b(-)CD27(+)DX5(hi)CD51(dull)CD127(dull)CD122(hi) stage, which contained the majority of Ly49G2(+) and D+ NK cells both in the spleen and BM. Even among wild-type splenic NK cells, IL-15-induced proliferation was most prominent in CD11b(-)DX5(hi) cells. Notably, IL-15-mediated preferential expansion (but not conversion from Ly49(-) cells) of Ly49(+) NK cells was observed in vitro only for NK cells in the spleen. These observations indicated the uneven distribution of NK cells of different developing stages with variable IL-15 responsiveness in these lymphoid organs. Immature NK cells in the spleen may contribute, as auxiliaries to those in BM, to the mature NK cell compartment through IL-15-driven extramarrow expansion under steady-state or inflammatory conditions. The Journal of Immunology, 2011, 187: 5162-5169.ArticleJOURNAL OF IMMUNOLOGY. 187(10):5162-5169 (2011)journal articl

Adjuvant Chemotherapy with Gemcitabine for Resected Biliary Tract Cancer: A Single-Arm Phase 2 Study

Article信州医学雑誌 65(2): 99-111(2017)journal articl

Finite temperature effects on the structural stability of Si-doped HfO2_{2} using first-principles calculations

The structural stabilities of the monoclinic and tetragonal phases of Si-doped HfO$_{2}$ at finite temperatures were analyzed using a computational scheme to assess the effects of impurity doping. The finite temperature effects considered in this work represented lattice vibration and impurity configuration effects. The results show that 6% Si doping stabilizes the tetragonal phase at room temperature, although a higher concentration of Si is required to stabilize the tetragonal phase at zero temperature. These data indicate that lattice vibration and impurity configuration effects are important factors determining structural stability at finite temperatures.Comment: 5 pages, 3 figure

Variation of Heating Efficiency of Magnetically Sheared CHS Plasmas by Polarization Control of 106GHz EC-Wave

To clarify the effect of polarization on electron cyclotron heating (ECH) in magnetized plasmas, experiment controlling the polarization of injected EC-waves is carried out in Compact Helical System (CHS). In the experiment, plasmas are generated and sustained only with 106.4 GHz ECH power. Magnetic field at the magnetic axis is 1.9 T so that the wave frequency is second harmonic. The optimum direction of linear polarization for the shortest time-delay of density start-up from the start of power injection and the optimum direction for the highest electron temperature and plasma stored energy during plasma duration show clear difference. The difference is attributed to the CHS magnetic configuration with strong shear and the plasma volume expansion from magnetic axis to the last closed flux surface

Clinical characteristics, prognosis, and surgical outcomes of patients with non-HBV and non-HCV related hepatocellular carcinoma: three-decade observational study

Abstract Background The incidence of non-hepatitis B virus, non-hepatitis C virus hepatocellular carcinoma (non-B non-C-HCC) is increasing worldwide. We assessed the clinical characteristics and surgical outcomes of non-B non-C-HCC, versus hepatitis B (HBV-HCC) and hepatitis C (HCV-HCC). Methods Etiologies, fibrosis stages, and survival outcomes were analyzed of 789 consecutive patients who underwent surgery from 1990 to 2020 (HBV-HCC, n = 149; HCV-HCC, n = 424; non-B non-C-HCC, n = 216). Results The incidence of hypertension and diabetes mellitus was significantly higher in patients with NON-B NON-C-HCC than in those with HBV-HCC and HCV-HCC. Significantly more advanced tumor stages were observed in patients with non-B non-C-HCC; however, better liver function and lower fibrosis stages were observed. Patients with non-B non-C-HCC had significantly worse 5-year overall survival than patients with HBV-HCC; overall survival was comparable between patients with non-B non-C-HCC and HCV-HCC. Patients with HCV-HCC had significantly worse 5-year recurrence-free survival than patients with HBV-HCC and non-B non-C-HCC. In patients with non-B non-C-HCC, overall survival was comparable among three periods (1990–2000, 2001–2010, and 2011–2020) despite significant improvement in patients with HBV-HCC and HCV-HCC. Conclusion The prognosis of non-B non-C-HCC was similar to that of HBV-HCC and HCV-HCC regardless of tumor progression at surgery. Patients with hypertension, diabetes mellitus, and dyslipidemia require careful systematic follow-up and treatment

IL-15–High-Responder Developing NK Cells Bearing Ly49 Receptors in IL-15 −/−

信州大学博士（医学）・学位論文・平成24年3月31日授与（甲第910号）・好沢克Originally published in The Journal of Immunology. Yoshizawa, K; Nakajima, S; Notake, T; Miyagawa, SI; Hida, S; Taki, S. 2011. IL-15-High-Responder Developing NK Cells Bearing Ly49 Receptors in IL-15(-/-) Mice . J. Immunol. Vol.187(10): pp5162-pp5169. Copyright (C) 2011 The American Association of Immunologists, Inc.In mice lacking IL-15, NK cell development is arrested at immature stages, providing an opportunity to investigate the earliest developing NK cells that would respond to IL-15. We show in this study that immature NK cells were present in the spleen as well as bone marrow (BM) and contained IL-15-high-responder cells. Thus, mature NK cells were generated more efficiently from IL-15(-/-) than from control donor cells in radiation BM chimeras, and the rate of IL-15-induced cell division in vitro was higher in NK cells in the spleen and BM from IL-5(-/-) mice than in those from wild-type mice. Phenotypically, NK cells developed in IL-15(-/-) mice up to the minor but discrete CD11b(-)CD27(+)DX5(hi)CD51(dull)CD127(dull)CD122(hi) stage, which contained the majority of Ly49G2(+) and D+ NK cells both in the spleen and BM. Even among wild-type splenic NK cells, IL-15-induced proliferation was most prominent in CD11b(-)DX5(hi) cells. Notably, IL-15-mediated preferential expansion (but not conversion from Ly49(-) cells) of Ly49(+) NK cells was observed in vitro only for NK cells in the spleen. These observations indicated the uneven distribution of NK cells of different developing stages with variable IL-15 responsiveness in these lymphoid organs. Immature NK cells in the spleen may contribute, as auxiliaries to those in BM, to the mature NK cell compartment through IL-15-driven extramarrow expansion under steady-state or inflammatory conditions. The Journal of Immunology, 2011, 187: 5162-5169.ArticleJOURNAL OF IMMUNOLOGY. 187(10):5162-5169 (2011)journal articl

Accuracy and limitations of preoperative assessment of longitudinal spread of perihilar cholangiocarcinoma

Objective: Although surgical resection offers the only chance of cure of perihilar cholangiocarcinoma and R1 resection has a poor prognosis, there is no consensus on optimal preoperative assessment of its longitudinal spread. We aimed to establish the optimal means of achieving this goal. Methods: This was a retrospective, single-center study of 61 patients who had undergone multi-detector row computed tomography, endoscopic retrograde cholangiography, intraductal ultrasonography, and mapping biopsy prior to resection of perihilar cholangiocarcinomas in our institute from January 2010 and December 2021. Results: The most accurate single methods for assessing longitudinal spread were intraductal ultrasonography and mapping biopsy (both 72.1%). A combination of all four assessment methods was accurate in 51 (83.6%) of our patients. Independent risk factors for inaccuracy were Bismuth–Corlette Type IV and high histologic-grade tumors. The R0 resection rate was higher with accurate than inaccurate assessments (90.2% vs. 30.0%, P < 0.001). R0 resection was associated with significantly better relapse-free survival than R1 resection (P = 0.006). However, overall survival did not differ between these groups. Conclusion: Preoperative assessment of longitudinal spread of perihilar cholangiocarcinomas by four different modalities is optimal, achieving 83.6% accuracy and a 90.2% R0 resection rate