34 research outputs found
Use of analysis of iron metabolism with CRP-dependent ferritin index and reticulocyte hemoglobin to determine treatment strategy for chemotherapy-related anemia
Preferential Interaction of α-tocopherol with PUFA-containing Lipids Characterized by Isothermal Titration Calorimetry
Hypochromic reticulocytes and ferritin index in the diagnosis of cancer- and chemotherapy-related anemia
Computational microscopy of cyclodextrin mediated cholesterol extraction from lipid model membranes
<p>Beta-cyclodextrins (beta-CDs) can form inclusion complexes with cholesterol, and are commonly used to manipulate cholesterol levels of biomembranes. In this work, we have used multiscale molecular dynamics simulations to provide a detailed view on the interaction between beta-CDs and lipid model membranes. We show that cholesterol can be extracted efficiently upon adsorption of beta-CD dimers at the membrane/water interface. However, extraction is only observed to occur spontaneously in membranes with high cholesterol levels. Free energy calculations reveal the presence of a kinetic barrier for cholesterol extraction in the case of low cholesterol content. Cholesterol uptake is facilitated in case of (poly) unsaturated lipid membranes, which increases the free energy of the membrane bound state of cholesterol. Comparing lipid/cholesterol compositions typical of liquid-disordered (L-d) and liquid-order (L-o) domains, we furthermore show that cholesterol is preferentially extracted from the disordered regions, in line with recent experimental data.</p>