Co-expression of recombinant single chain variable fragment recognizing blood antigen fused with sumo and chaperones in Escherichia coli

Single chain variable fragments (scFv) have widely been used in research, diagnosis and treatment, but the scFv is considered as difficult protein for expression in E. coli. In previous studies, we expressed a construction of recombinant single chain variable fragments again antigen specific for blood type A (antiA-scFv) individually or fused with Trx or SUMO. However, soluble fraction was low abandant and only approximately 40% when fused with Trx, the other cases were expressed in form of inclusion body. Therefore, it was difficult for purification, refolding and activity assesment. In thispaper, we demonstrated a suitable construction for soluble production of antiA-scFv fused with SUMO (SM/antiA-scFv) in presence of chaparones. Under fermentation with 0.1 mM IPTG at 20oC, the SM/antiA-scFv was entirely expressed in soluble form. Importantly, after cleavage from SUMO with SUMOprotease, antiA-scFv was still maintained in the supernatant fraction. Therefore, it can help ensure bioactivity and is useful for purification process. To the best of our knowledge, this is the first report showing soluble recombinant scFv fused with SUMO in presence of chaperone for determination of blood group antigens. Thus, this result facilitates the optimal study of soluble expression, purification and bioactivity determination of the antiA-scFv recombinant antibody.

Expression of the recombinant single chain variable fragments recognizing blood antigen fused with thioredoxin in Escherichia coli

The technology of recombinant single chain variable fragments (scFvs) expression has been used in research, diagnosis and treatment of diseases. In the previous study, we studied the expression of a recombinant single chain variable fragment recognizing blood A antigen (antiA-scFv) in E. coli. However, the protein was insoluble form resulting in difficulty for purification, refolding and activity assesment. Here, we present the study on fused expression of the recombinant scFv -specific blood A antigen with thioredoxin (Trx) in the expression vector pET32a (+). The results showed that the Trx/antiA-scFv fusion protein was expressed with molecular weight of 49 kDa in a soluble form reaching 40% of the total recombinant protein. This result facilitates the optimal condition of soluble protein expression, purification and bioactivity determination of the antiA-scFv recombinant antibody.

Prevalence of Accidents and Injuries and Related Factors of Fishermen Fishing Offshore in the North of Vietnam

Introduction: Seafaring, particularly offshore fishing, exposes fishermen to various occupational risks leading to diseases and injuries. This study aimed to determine the prevalence of occupational risks, injury accidents, and contributing factors among offshore fishers in North Vietnam, to develop evidence-based recommendations to enhance their safety and well-being. Methods: A cross-sectional study was conducted involving 420 fishermen with a minimum of two years of experience. Interviews were conducted between 2018 and 2020. Results: The findings indicated that there is a 41.7% prevalence of accidents and an average injury rate of 280.2 per person per year. Most incidents occurred at night with 104 cases (59.4%), slips and falls 48 cases (27.4%), broken winch lines 40 cases (22.9%), and ship collisions 14 cases (8.0%) being the primary causes. The most common injuries included soft wounds in 92 cases (52.5%) and sprains/dislocations in 14 cases (8.0%). Fishermen with fewer than 10 years of experience exhibited a higher accident risk (odds ratio = 1.54; 95% confidence interval: 1.05-2.72), as did those in the role of a fisherman (odds ratio: 1.68; CI: 0.97-2.94) and those working without labor protection (odds ratio: 3.68; CI: 1.05-12.93). Conclusion: Lack of labor protection equipment increased the risk by 3.68 times, and fishermen in the friend group had a 2.02 times higher risk of injury. Addressing these risks requires adherence to labor protection regulations and safe working procedures

A problem of self-isolation in Japan: The relationship between self-isolation and COVID-19 community case

Background: The Japanese government advised mild or asymptomatic　coronavirus disease-2019 (COVID-19) cases to self-isolate at home, while more severe individuals were treated at health posts. Poor compliance with self-isolation could be a potential reason for the new outbreak. Our study aimed to find out the correlation between the rising new cases of COVID-19 and home-based patients in Japan.Methods: A secondary data analysis study was conducted with the data from COVID-19-involved databases collected from Johns Hopkins University, Japanese Ministry of Health, Labour and Welfare, and Community Mobility Reports of Google. New community cases, stringency index, number of tests, and active cases were analyzed. Using a linear regression model, an independent variable was utilized for a given date to predict the future number of community cases.Results: Research results show that outpatient cases, the stringency, and Google Mobility Trend were all significantly associated with the number of COVID-19 community cases from the sixth day to the ninth day. The model predicting community cases on the eighth day (R2=0.8906) was the most appropriate showing outpatients, residential index, grocery and pharmacy index, retail and recreation index, and workplaces index were positively related (β1=24.2, 95% CI: 20.3–26.3, P<0.0001; β2=277.7, 95% CI: 171.8–408.2, P<0.0001; β3=112.4, 95% CI: 79.8–158.3, P<0.0001; β4=73.1, 95% CI: 53- 04.4, P<0.0001; β5=57.2, 95% CI: 25.2–96.8, P=0.001, respectively). In contrast, inpatients, park index, and adjusted stringency index were negatively related to the number of community cases (β6=-2.8, 95% CI: -3.9 – -1.6, P<0.0001; β7=-33, 95% CI: -43.6 – -27, P<0.0001; β8=-14.4, 95% CI: -20.1– -12, P<0.0001, respectively).Conclusion: Outpatient cases and indexes of Community Mobility Reports were associated with COVID-19 community cases

A Large-Scale Study of a Sleep Tracking and Improving Device with Closed-loop and Personalized Real-time Acoustic Stimulation

Various intervention therapies ranging from pharmaceutical to hi-tech tailored solutions have been available to treat difficulty in falling asleep commonly caused by insomnia in modern life. However, current techniques largely remain ill-suited, ineffective, and unreliable due to their lack of precise real-time sleep tracking, in-time feedback on the therapies, an ability to keep people asleep during the night, and a large-scale effectiveness evaluation. Here, we introduce a novel sleep aid system, called Earable, that can continuously sense multiple head-based physiological signals and simultaneously enable closed-loop auditory stimulation to entrain brain activities in time for effective sleep promotion. We develop the system in a lightweight, comfortable, and user-friendly headband with a comprehensive set of algorithms and dedicated own-designed audio stimuli. We conducted multiple protocols from 883 sleep studies on 377 subjects (241 women, 119 men) wearing either a gold-standard device (PSG), Earable, or both concurrently. We demonstrate that our system achieves (1) a strong correlation (0.89 +/- 0.03) between the physiological signals acquired by Earable and those from the gold-standard PSG, (2) an 87.8 +/- 5.3% agreement on sleep scoring using our automatic real-time sleep staging algorithm with the consensus scored by three sleep technicians, and (3) a successful non-pharmacological stimulation alternative to effectively shorten the duration of sleep falling by 24.1 +/- 0.1 minutes. These results show that the efficacy of Earable exceeds existing techniques in intentions to promote fast falling asleep, track sleep state accurately, and achieve high social acceptance for real-time closed-loop personalized neuromodulation-based home sleep care.Comment: 33 pages, 8 figure

Results of acute cerebral infarction treatment with hyperbaric oxygen therapy, 2020–2022

Background: Cerebral stroke is the third leading cause of death after cardiovascular disease, cancerand the leading cause of disability for patients. Hyperbaric oxygen is a non-drug treatment that hasthe potential to improve brain function for patients with ischaemic stroke. The objective of this study wasto evaluate the results of treatment of acute cerebral infarction with hyperbaric oxygen therapy (HBOT).Materials and methods: This was a case-control study. One hundred ninety-five patients diagnosed withcerebral infarction, with signs of onset within 24 hours, were treated at the Centre for Underwater Medicineand Hyperbaric Oxygen of Vietnam National Institute of Maritime Medicine during the period from January2020 to December 2022. Study group included 100 patients with acute cerebral infarction treated witha combination of HBOT and medication and reference group included 95 patients treated by medicationonly (antiplatelets drugs, statins, control of associated risks factors)Results: After 7 days of treatment with hyperbaric oxygen (HBO), symptoms such as headache, dizziness,nausea, sensory disturbances, and Glasgow score of the study group improved better than that of the referencegroup (p &lt; 0.01). Movement recovery in the study group was better than the reference group:the percentage of patients with mild and moderate paralysis in the study group increased higher than thatof the reference group (86.0% and 68.4%), the degree of complete paralysis of the study group decreasedmore than that of the reference group (14.0% and 31.6%). The degree of independence in daily activitiesin the study group was better than the reference group. In the study group, the percentage of patients withcomplete independence in daily life increased from 27.0% to 84.0%. In the reference group, the rate of patientswho were independent in their daily activities increased from 37.9% to 51.6%. The average numberof treatment days of the study group was 10.32 ± 2.41 days and it the reference group 14.51 ± 3.24 days.Conclusions: Hyperbaric oxygen therapy is a non-drug treatment with many good effects in the treatmentof cerebral infarction, especially acute cerebral infarction. HBOT reduces and improves functional symptoms,improves mobility, and reduces treatment time for patients

EFFECT OF SOME DISPERSING AUXILIARIES ON NANOSILICA DISPERSION INTO PASSIVE CHROME TRIVALENT SOLUTION

In this paper, the dispersion of nanosilica in conversion solution trivalent chromium using variety of dispersion aids namely: SDS, PVP, AE-7, OP-10 and epomin. The results showed that the nanosilica could disperse in conversion solution in low pH (pH = 1.5). The solution containing nanosilica was uniform, having no agglomeration with SDS, PVP and AE-7 after 7-day preparation. The results of zeta potential and size distribution illustrated that AE-7 was the most effective for nanosilica dispersion in passive solution trivalent chromium with medium size of nanosilica in C2-nanosilica solution using AE-7 equal ~ 60 nm. However, there was a much diffirence size between the size of nanosilica in passive solution and initial nanosilica. As a result, nanosilica could disperse in passive solution at low pH with AE-7 but this was not effective enough to held dispersed solution in stable state

NGHIÊN CỨU TỐI ƯU ĐIỀU KIỆN BIỂU HIỆN INTERLEUKIN-3 NGƯỜI DUNG HỢP VỚI PelB TRONG E. coli

Interleukin-3 (IL-3) is a multifunctional cytokine which modulates the proliferation, differentiation and maturation of various types of hematopoietic cells. Gene coding for IL-3 linked with pelB signal were incorporated into pET22b(+) for  expression of il3 gene in E. coli BL21; but IL-3 was synthesized at very low levels and still in fusion with PelB. To facilitate purification process, finding the appropriate fermentation conditions plays a key role in order to enhance gene il-3 expression and cleavage of PelB. In this study, we have optimized the conditions for the expression of IL-3, which included E. coli host strain JM109, LB cultivation medium, induction temperature was 25oC; induction with 0.05 mM IPTG at OD600 = 1. The cell biomass increases at about 2.3 times after optimization. IL-3 protein was expressed in the form of inclusion body and the PelB signal was cleaved. This result is conducive for purification of large amount of IL-3 to determine characteristics of protein. Interleukin-3 người (IL-3) là một cytokine đa chức năng tham gia vào các quá trình tự đổi mới, nhân lên, biệt hóa và trưởng thành của nhiều loại tế bào máu. Sau khi đưa gen il-3 gắn thêm tín hiệu tiết pelB vào vector pET22b(+) và tiến hành biểu hiện ở chủng E. coli BL21, chúng tôi nhận thấy IL-3 được tổng hợp ở mức rất thấp và còn gắn với PelB. Để thuận tiện cho khâu tinh sạch thì vấn đề then chốt là nghiên cứu tìm ra các điều kiện phù hợp làm tăng lượng IL-3 được tổng hợp, đồng thời cắt được PelB. Trong nghiên cứu này, chúng tôi so sánh khả năng sinh tổng hợp IL-3 của các chủng E. coli BL21, JM109, Soluble và Rossetta2; sau đó tối ưu hóa điều kiện biểu hiện gen il-3 về thành phần môi trường, nhiệt độ, nồng độ IPTG, thời điểm cảm ứng và kiểm tra trạng thái tồn tại của IL-3. Kết quả thu được cho thấy gen il-3 biểu hiện tốt và ổn định nhất ở chủng E. coli JM109. Dưới các điều kiện lên men thích hợp trong môi trường LB, ở 25oC, cảm ứng 0,05 mM IPTG tại OD600=1, IL-3 biểu hiện tốt, cắt khỏi PelB và tồn tại ở trạng thái không tan trong tế bào chất. Sinh khối tế bào tăng lên khoảng 2,3 lần sau khi tối ưu. Kết quả này là tiền đề cho bước tinh sạch lượng lớn IL-3 cho nghiên cứu tính chất của protein

Synthesis, Biological Evaluation, and Molecular Modeling Studies of 1-Aryl-1H-pyrazole-Fused Curcumin Analogues as Anticancer Agents

Addressing the growing burden of cancer and the shortcomings of chemotherapy in cancer treatment are the current research goals. Research to overcome the limitations of curcumin and to improve its anticancer activity via its heterocycle-fused monocarbonyl analogues (MACs) has immense potential. In this study, 32 asymmetric MACs fused with 1-aryl-

THÀNH PHẦN LOÀI VÀ SINH KHỐI CỎ BIỂN TẠI ĐẦM SAM CHUỒN VÀ HÀ TRUNG, TỈNH THỪA THIÊN HUẾ

This research was conducted at Sam Chuon and Ha Trung lagoons, Thua Thien Hue province. The research has identified 5 seagrass species including Halophila beccarii, Zostera japonica, Halodule pinifolia, Halodule uninervis and Ruppia maritima in Sam Chuon and Ha Trung lagoons. The total biomass of seagrass fluctuates seasonally. In the dry season, the total biomass of seagrass at research station S6 is highest at 136.23 g/m2 and lowest at research stations H7 and H8 at 0.64 g/m2. In the rainy season, sampling station S6 also recorded the highest total biomass with a value of 113.20 g/m2, the lowest at two stations H7 and H8 with 0.64 g/m2. Halodule uninervis and Ruppia maritima are the dominant species, which appeared in 11/14 stations in total. While Ruppia maritima is only recognized at 3/14 stations in total.Nghiên cứu này được tiến hành khảo sát tại đầm Sam Chuồn và Hà Trung thuộc hệ đầm phá Tam Giang - Cầu Hai, tỉnh Thừa Thiên Huế. Nghiên cứu đã xác định được năm loài cỏ biển là Halophila beccarii (cỏ Nàn), Zostera japonica (cỏ Lươn nhật), Halodule pinifolia (cỏ Hẹ tròn), Halodule uninervis (cỏ Hẹ ba răng) và loài Ruppia maritima (cỏ Kim biển) phân bố tại đầm Sam Chuồn và Hà Trung. Tổng sinh khối của cỏ biển có sự biến động theo mùa. Vào mùa khô, tổng sinh khối của cỏ biển tại trạm nghiên cứu S6 cao nhất với 136,23 g/m2 và thấp nhất tại trạm nghiên cứu H7 và H8 với 0,64 g/m2. Vào mùa mưa tại trạm thu mẫu S6 cũng ghi nhận tổng sinh khối của cỏ biển cao nhất với giá trị đạt được 113,20 g/m2, thấp nhất tại hai trạm nghiên cứu H7 và H8 với 0,64 g/m2. Loài Halodule uninervis (cỏ Hẹ ba răng) chiếm ưu thế hơn cả với sự ghi nhận tại 11/14 trạm thu mẫu. Và loài Ruppia maritima (cỏ Kim biển) chỉ ghi nhận xuất hiện tại 3/14 trạm thu mẫu