Biotreatment of Winery Wastewater Using a Hybrid System Combining Biological Trickling Filters and Constructed Wetlands

The objective of this work was to determine the ability of a pilot-scale hybrid system to treat real (non-synthetic) winery wastewater. The experimental treatment system consisted of two stages: An attached growth pilot-scale bioreactor (biological trickling filter with plastic support material) was initially used to remove a significant amount of dissolved chemical oxygen demand (d-COD) from winery wastewater, and then a pilot-scale, horizontal subsurface flow constructed wetland (CW) was examined as a post-treatment step for further d-COD removal. Results from the biofilter revealed that the recirculation rate of 1.0 L/min lead to higher d-COD removal rates than that of 0.5 L/min for all feed d-COD concentrations tested (3500, 7500, 9000 and 18,000 mg d-COD/L). Experiments in the CW were performed using feed d-COD concentrations of about 1500 mg/L (equivalent to biofilter effluent when initial filter feed d-COD concentrations are 18,000 mg/L). The wetland polishing stage managed to further remove d-COD and produced effluent concentrations below current legislation limits for safe disposal. Furthermore, the presence of zeolite in CW (one third of the length of CW) enhanced ammonium removal. The experimental results indicate that the combination of a biological trickling filter and a constructed wetland could effectively treat effluents originating from small wineries typical of the Mediterranean region

Zeolite as a Potential Medium for Ammonium Recovery and Second Cheese Whey Treatment

The efficiency of natural zeolite to remove ammonium from artificial wastewater (ammonium aqueous solutions) and to treat second cheese whey was examined, aiming to recover nitrogen nutrients that can be used for further applications, such as slow-release fertilizers. Sorption experiments were performed using artificial wastewater and zeolite of different granulometries (i.e., 0.71–1.0, 1.8–2.0, 2.0–2.8, 2.8–4.0, and 4.0–5.0 mm). The granulometry of the zeolite had no significant effect on its ability to absorb ammonium. Nevertheless, smaller particles (0.71–1.0 mm) exhibited quicker NH4+-N adsorption rates of up to 93.0% in the first 10 min. Maximum ammonium removal efficiency by the zeolite was achieved at ammonium concentrations ranging from 10 to 80 mg/L. Kinetic experiments revealed that chemisorption is the mechanism behind the adsorption process of ammonium on zeolite, while the Freundlich isotherm model fitted the experimental data well. Column sorption experiments under batch operating mode were performed using artificial wastewater and second cheese whey. Column experiments with artificial wastewater showed high NH4+-N removal rates (over 96% in the first 120 min) for all granulometries and initial NH4+-N concentrations tested (200 and 5000 mg/L). Column experiments with second cheese whey revealed that natural zeolite can remove significant organic loads (up to 40%, 14.53 mg COD/g of zeolite) and NH4+-N (about 99%). For PO43−-P, the zeolite appeared to saturate after day 1 of the experiments at a removal capacity of 0.15 mg P/g of zeolite. Desorption experiments with water resulted in low NH4+-N and PO43−-P desorption rates indicating that the zeolite could be used as a substrate for slow nitrogen release in soils

Biotreatment of Poultry Waste Coupled with Biodiesel Production Using Suspended and Attached Growth Microalgal-Based Systems

Poultry litter extract (PLE) was treated using a microbial consortium dominated by the filamentous cyanobacterium Leptolyngbya sp. in synergy with heterotrophic microorganisms of the poultry waste. Laboratory- and pilot-scale experiments were conducted under aerobic conditions using suspended and attached growth photobioreactors. Different dilutions of the extract were performed, leading to different initial pollutant (nitrogen, phosphorus, dissolved chemical oxygen demand (d-COD), total sugars) concentrations. Significant nutrient removal rates, biomass productivity, and maximum lipid production were determined for all the systems examined. Higher d-COD, nitrogen, phosphorus, and total sugars removal were recorded in the attached growth reactors in both laboratory- (up to 94.0%, 88.2%, 97.4%, and 79.3%, respectively) and pilot-scale experiments (up to 82.0%, 69.4%, 81.0%, and 83.8%, respectively). High total biomass productivities were also recorded in the pilot-scale attached growth experiments (up to 335.3 mg L−1d−1). The produced biomass contained up to 19.6% lipids (w/w) on a dry weight basis, while the saturated and monounsaturated fatty acids accounted for more than 70% of the total fatty acids, indicating a potential biodiesel production system. We conclude that the processing systems developed in this work can efficiently treat PLE and simultaneously produce lipids suitable as feedstock in the biodiesel manufacture