Elevated pCO2 enhances bacterioplankton removal of organic carbon.

Factors that affect the removal of organic carbon by heterotrophic bacterioplankton can impact the rate and magnitude of organic carbon loss in the ocean through the conversion of a portion of consumed organic carbon to CO2. Through enhanced rates of consumption, surface bacterioplankton communities can also reduce the amount of dissolved organic carbon (DOC) available for export from the surface ocean. The present study investigated the direct effects of elevated pCO2 on bacterioplankton removal of several forms of DOC ranging from glucose to complex phytoplankton exudate and lysate, and naturally occurring DOC. Elevated pCO2 (1000-1500 ppm) enhanced both the rate and magnitude of organic carbon removal by bacterioplankton communities compared to low (pre-industrial and ambient) pCO2 (250 -~400 ppm). The increased removal was largely due to enhanced respiration, rather than enhanced production of bacterioplankton biomass. The results suggest that elevated pCO2 can increase DOC consumption and decrease bacterioplankton growth efficiency, ultimately decreasing the amount of DOC available for vertical export and increasing the production of CO2 in the surface ocean

Post Feed-in Scheme Photovoltaic System Feasibility Evaluation in Italy: Sicilian Case Studies

Thanks to national energy policies, over recent years the Italian photovoltaic (PV) sector has undergone an extraordinary growth, also affecting the primary sector. In this context, Mediterranean greenhouses are well-adapted to photovoltaic systems because they represent one of the most energy-intensive sectors in agriculture. The Italian feed-in scheme ended at the beginning of 2013, making it necessary to investigate the feasibility of photovoltaic systems devoid of any electricity production-related incentives. In this paper, production cost and profitability analyses of photovoltaic electricity have been conducted, considering Mediterranean solar greenhouses in which, thanks to net metering, all the electricity produced by photovoltaic panels is self-consumed. Our results showed that grid parity is already reached for Sicilian PV systems with a capacity greater than 50 kW. Moreover, net present value, internal rate of return and discounted payback time all demonstrate the high economic convenience of all the photovoltaic investments analyzed, due to the huge savings on energy expenditures

Systematic review on spheroids from adipose‐derived stem cells: Spontaneous or artefact state?

Three‐dimensional (3D) cell cultures represent the spontaneous state of stem cells with specific gene and protein molecular expression that are more alike the in vivo condition. In vitro two‐dimensional (2D) cell adhesion cultures are still commonly employed for various cellular studies such as movement, proliferation and differentiation phenomena; this procedure is standardized and amply used in laboratories, however their representing the original tissue has recently been subject to questioning. Cell cultures in 2D require a support/substrate (flasks, multiwells, etc.) and use of fetal bovine serum as an adjuvant that stimulates adhesion that most likely leads to cellular aging. A 3D environment stimulates cells to grow in suspended aggregates that are defined as “spheroids.” In particular, adipose stem cells (ASCs) are traditionally observed in adhesion conditions, but a recent and vast literature offers many strategies that obtain 3D cell spheroids. These cells seem to possess a greater ability in maintaining their stemness and differentiate towards all mesenchymal lineages, as demonstrated in in vitro and in vivo studies compared to adhesion cultures. To date, standardized procedures that form ASC spheroids have not yet been established. This systematic review carries out an in‐depth analysis of the 76 articles produced over the past 10 years and discusses the similarities and differences in materials, techniques, and purposes to standardize the methods aimed at obtaining ASC spheroids as already described for 2D cultures

Cities' Identity Through Architecture and Art

Intended to be a guide for academics, scholars, and interested leaders, this book was designed to critically assess issues related to architectural identity, the city as a scene, the city as an organism, the city as a subject, and the planning or rather approaching of one. A pressing issue for many researchers in the field, the book discusses the negative repercussions resulting from globalization. Studies have indicated that globalization, despite all the positive effects, has resulted in a loss of identity within a city. As a city develops over time, its identity is evolving as well and may even be lost due to rapid and constant changes it is subjected to. Discussed as well are examples and tendencies in dealing with urban identities as well as the transformation of cities and urban cultures mentioned in terms of form, identity, and art. This book is a combination of innovative research submitted to a conference on Cities’ Identity Through Architecture and Arts (CITAA) whereas scholars from all over the world gather in one venue to discuss cultural, historical, and economic issues of the city. Thus, the book offers a collective and global solution that is applicable on a universal level. The research presented in this book was conducted by authors, or rather participants of the conference from, three different continents of the world and organized by IEREK. It was a distinct opportunity for them to share their thoughts with leading scholars and professionals in the field of Architecture, Arts, and Planning. The research and materials in this book are directed at those who are actively engaged in the decision-making processes and to a heterogeneous audience who has an interest to critically examine all the new literature available in the field. A special word of thanks should be made to the editors of this book and to all the authors and co-authors of the chapters who collectively provided the academic community with unique and increasingly valuable literature

economic sustainability of early potato production in the mediterranean area

The interactions between a given commercial enterprise, its territory and development, have become a key issue in recent years. Theories of economic development tell us that a territory may undergo development provided viable, economically sustainable commercial activities exist. Based on the latter postulate, the present paper analyzes the economic sustainability of farming enterprises growing early potatoes in a Mediterranean setting. In particular, the study was conducted in south-eastern Sicily, island in the centre of the Mediterranean. The results underscore their modest profitability, mainly due to a lack of entrepreneurial strategies aimed at enhancing production. This situation leads to shortcomings in terms of economic viability of the considered activities, which according to economic theory underlie an ultimate impoverishment of the territory

economic analysis of process innovations in the management of olive farms

Within a business enterprise, process innovations l ead to reduced production costs and to increased profit margins. In this study, we shall examine an olive farm that operates in a perfectly competitive market that has introduced a process innovation so as to contain labour costs and therefore production costs. So, the paper aimed at comparing economic co mpetitiveness of an olive farm that introduced mechanical olive pickers (process innovation) for h arvest respect to manual harvest. In the case under scrutiny, for the same price, the reduced productio n cost determined a shift from a situation of being at a loss to one of profit for the business. Economic analysis showed that introduction of mechanical olive pickers can determine a competitive advantage in small and medium-sized olive farms of Mediterranean areas which decide to mechanize the harvest

economic evaluation of aquaculture investments under conditions of risk and uncertainty in the mediterranean sea

This study aims to analyze, from a technical and ec onomic point of view, the choice between alternative investments in aquaculture under condit ions of risk and uncertainty. In particular, a comparative analysis has been conducted between an inshore and an offshore farm producing European sea bass in the Mediterranean Sea. The app lied model refers to the theory of subjective probability, assigning a different probability to e xpected incomes during the investment period. Resul ts show an economic convenience of inshore with respect to offshore farming

Cyto/Biocompatibility of Dopamine Combined with the Antioxidant Grape Seed-Derived Polyphenol Compounds in Solid Lipid Nanoparticles

none10The loss of nigrostriatal neurons containing dopamine (DA) together with the “mitochondrial dysfunction” in midbrain represent the two main causes related to the symptoms of Parkinson’s disease (PD). Hence, the aim of this investigation is to co-administer the missing DA and the antioxidant grape seed-derived proanthocyanidins (grape seed extract, GSE) in order to increase the levels of the neurotransmitter (which is unable to cross the Blood Brain Barrier) and reducing the oxidative stress (OS) related to PD, respectively. Methods: For this purpose, we chose Solid Lipid Nanoparticles (SLN), because they have been already proven to increase DA uptake in the brain. DA-SLN adsorbing GSE (GSE/DA-SLN) were formulated and subjected to physico-chemical characterization, and their cytocompatibility and protection against OS were examined. Results: GSE was found on SLN surface and release studies evidenced the efficiency of GSE in preventing DA autoxidation. Furthermore, SLN showed high mucoadhesive strength and were found not cytotoxic to both primary Olfactory Ensheathing and neuroblastoma SH-SY5Y cells by MTT test. Co-administration of GSE/DA-SLN and the OS-inducing neurotoxin 6-hydroxydopamine (100 μM) resulted in an increase of SH-SY5Y cell viability. Conclusions: Hence, SLN formulations containing DA and GSE may constitute interesting candidates for non-invasive nose-to-brain delivery.openAdriana Trapani, Lorenzo Guerra, Filomena Corbo, Stefano Castellani, Enrico Sanna, Loredana Capobianco, Anna Grazia Monteduro, Daniela Erminia Manno, Delia Mandracchia, Sante Di Gioia and Massimo ConeseTrapani, Adriana; Guerra, Lorenzo; Corbo, Filomena; Castellani, Stefano; Sanna, Enrico; Capobianco, Loredana; Monteduro, ANNA GRAZIA; Manno, Daniela Erminia; Mandracchia, Delia; Di Gioia and Massimo Conese, Sant

Native Mitral Valve Endocarditis Caused by Neisseria elongata subsp. nitroreducens in a Patient with Marfan Syndrome: First Case in Italy and Review of the Literature

Neisseria elongata (NE) is an aerobic Gram-negative organism that constitutes part of the commensal human normal oropharyngeal flora. Although previously considered not to be pathogenic, it has been recognized as an occasional cause of significant infections in humans. We report here the first case in Italy of infective endocarditis of a native prolapsing mitral valve in a patient with Marfan syndrome, caused by NE subspecies nitroreducens which has been rarely isolated from clinical specimens. The culprit organism has been confirmed by mass spectrometry directly from the positive blood culture, as previously reported. The amplified gene has been deposited in GenBank under accession number KT591873. In spite of the reported aggressive nature of NE, clinical remission was promptly obtained, there being no requirement for surgery