Performance of food–feed maize and cowpea cultivars under monoculture and intercropping systems: Grain yield, fodder biomass, and nutritive value

Livestock feeding in Burkina Faso is characterized by a recurrent deficit in both the quality and the quantity of fodder during the dry season, which affects animal performance. To overcome this, quality fodder/forage production is an alternative. Therefore, this study evaluated food- and feed-improved cultivars of maize and cowpea in intercropping trials using the “mother and baby trials” approach with crop–livestock farmers. The mother trial comprised a randomized block design with eight treatments and four replicates: two cowpea (KVx745-11P and Tiligré) and two maize cultivars (Barka and Espoir), and grown under two cropping systems (monoculture and intercropping). Baby trials were established on-farm and involved 30 farmers during two seasons (2019 and 2020) in four villages in the South Sudan zone of Burkina Faso. Data were collected on (1) weed density and biomass, (2) grain yield and fodder biomass, (3) intercropping efficiency, and (4) fodder nutritive value. Data were analyzed using ANOVA and the least significant difference (LSD) means separation at a 5% threshold. The results revealed that maize and cowpea intercropping significantly reduced weed biomass (p ≤ 0.05). In monoculture, the maize cultivar Barka produced a greater grain yield (4980 kg/ha) and fodder biomass [6259 kg dry matter (DM)/ha] than the cultivar Espoir, which produced a grain yield of 2581 kg/ha and fodder biomass of 4952 kg DM/ha. The cowpea cultivars, KVx745-11P and Tiligré, were similar (p ≥ 0.05) in terms of fodder biomass (2435–2820 kg DM/ha) and grain yield (1152–1163 kg/ha). For the intercropping system, land equivalent ratios for fodder biomass (1.18:1.41) and grain yield (1.02:1.44) were greater than 1; intercropping also had better productivity system indexes than the monoculture cropping system. The crude protein concentration of fodder was greater for Barka maize (9.5%–9.8%) than for Espoir maize (8.5%–8.7%). The crude protein concentration was greater for cowpea KVx745-11P (19%–21.8%) than for cowpea Tiligré (15%–17%). Intercropping both Barka maize and cowpea KVx745-11P was the most productive cropping system for maximizing grain and fodder production for crop–livestock farmers in the South Sudan zone of Burkina Faso

Analysis of the Genetic Variation of the Fruitless Gene within the Anopheles gambiae (Diptera: Culicidae) Complex Populations in Africa

Targeting genes involved in sexual determinism, for vector or pest control purposes, requires a better understanding of their polymorphism in natural populations in order to ensure a rapid spread of the construct. By using genomic data from An. gambiae s.l., we analyzed the genetic variation and the conservation score of the fru gene in 18 natural populations across Africa. A total of 34,339 SNPs were identified, including 3.11% non-synonymous segregating sites. Overall, the nucleotide diversity was low, and the Tajima’s D neutrality test was negative, indicating an excess of low frequency SNPs in the fru gene. The allelic frequencies of the non-synonymous SNPs were low (freq < 0.26), except for two SNPs identified at high frequencies (freq > 0.8) in the zinc-finger A and B protein domains. The conservation score was variable throughout the fru gene, with maximum values in the exonic regions compared to the intronic regions. These results showed a low genetic variation overall in the exonic regions, especially the male sex-specific exon and the BTB-exon 1 of the fru gene. These findings will facilitate the development of an effective gene drive construct targeting the fru gene that can rapidly spread without encountering resistance in wild populations

Genetic Diversity and Population Structure of the Invasive Oriental Fruit Fly, Bactrocera dorsalis (Diptera: Tephritidae) in Burkina Faso

Bactrocera dorsalis Hendel is a highly invasive horticultural pest that is of major economic importance worldwide. In Burkina Faso, it is one of the main insect pests that affects the production and exportation of mangos. Understanding the biology and the genetic dynamics of this insect pest provides crucial information for the development of effective control measures. The aim of this study was to understand the distribution, diversity, and genetic structure of B. dorsalis in Burkina Faso. Male flies were collected transversally in Burkina Faso and analyzed by PCR using 10 microsatellite markers. The results showed an abundance of B. dorsalis varying from 87 to 2986 flies per trap per day at the different sampling sites. The genetic diversity was high at all sites, with an average Shannon’s Information Index (I) of 0.72 per site. The gene flow was high between study populations and ranged from 10.62 to 27.53 migrants. Bayesian admixture analysis showed no evidence of structure, while Discriminant Analysis of Principal Components identified three weakly separated clusters in the population of B. dorsalis in Burkina Faso. The results of this study could be used to optimize the effectiveness of current control interventions and to guide the implementation of new, innovative, and sustainable strategies

Genetic Diversity and Population Structure of the Invasive Oriental Fruit Fly, Bactrocera dorsalis (Diptera: Tephritidae) in Burkina Faso

Bactrocera dorsalis Hendel is a highly invasive horticultural pest of major economic importance worldwide. In Burkina Faso, it is one of the main insect pests constraining the mango production and export. Understanding the biology and the genetic dynamics of this insect pest provide cru-cial information for the development of effective control measures. The aim of this study was to understand the distribution, diversity and genetic structure of B. dorsalis in Burkina Faso. Male flies were collected transversally in Burkina Faso and analyzed by PCR using 10 microsatellite markers. The results showed an abundance of B. dorsalis varying from 87 to 2986 flies per trap per day in the different sampling sites. Genetic diversity was high in all sites with an average Shan-non's Information Index (I) of 0.72 per site. Gene flow was high between study populations and ranged from 10.62 to 27.53 number of migrants. Bayesian admixture analysis showed no evi-dence of structure while Discriminant Analysis of Principal Components identified three weakly separated clusters in the population of B. dorsalis in Burkina Faso. The results of this study could be used to optimize the effectiveness of current control interventions and to guide the imple-mentation of new, innovative and sustainable strategies