85 research outputs found
System-Wide Immunohistochemical Analysis of Protein Co-Localization
Background: The analysis of co-localized protein expression in a tissue section is often conducted with immunofluorescence histochemical staining which is typically visualized in localized regions. On the other hand, chromogenic immunohistochemical staining, in general, is not suitable for the detection of protein co-localization. Here, we developed a new protocol, based on chromogenic immunohistochemical stain, for system-wide detection of protein co-localization and differential expression. Methodology/Principal Findings: In combination with a removable chromogenic stain, an efficient antibody stripping method was developed to enable sequential immunostaining with different primary antibodies regardless of antibody’s host species. Sections were scanned after each staining, and the images were superimposed together for the detection of protein co-localization and differential expression. As a proof of principle, differential expression and co-localization of glutamic acid decarboxylase67 (GAD67) and parvalbumin proteins was examined in mouse cortex. Conclusions/Significance: All parvalbumin-containing neurons express GAD67 protein, and GAD67-positive neurons that do not express parvalbumin were readily visualized from thousands of other neurons across mouse cortex. The method provided a global view of protein co-localization as well as differential expression across an entire tissue section. Repeate
Influence du niveau nutritionnel sur les neurones à corticolibérine (CRF) du noyau paraventriculaire. Etude immunohistochimique chez la brebis
National audienc
Presence of galanin in dopaminergic neurons of the sheep infundibular nucleus: a double staining immunohistochemical study
International audienc
Immunohistochemical characterization of the sheep suprachiasmatic nucleus
International audienc
Undernutrition and over-feeding effects on the galanin (GAL) immunoreactivity in the preoptic area and infundibular nucleus of ewes
International audienc
Immunohistochemical characterization of the sheep suprachiasmatic nucleus
International audienc
Sensitivity of galanin- and melanin concentrating hormone-containing neurones to nutritional status : an immunohistochemical study in the ovariectomized ewe
International audienc
In vivo activation of the interleukin-6 receptor/gp130 signaling pathway in pituitary corticotropes of lipopolysaccharide-treated rats
International audienc
Neuropeptides and nutrition in the sheep hypothalamus: a short review
International audienc
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