A diet based on multiple functional concepts improves cardiometabolic risk parameters in healthy subjects

Background: Different foods can modulate cardiometabolic risk factors in persons already affected by metabolic alterations. The objective of this study was to assess, in healthy overweight individuals, the impact of a diet combining multiple functional concepts on risk markers associated with cardiometabolic diseases (CMD). Methods: Fourty-four healthy women and men (50-73 y.o, BMI 25-33, fasting glycemia <= 6.1 mmol/L) participated in a randomized crossover intervention comparing a multifunctional (active) diet (AD) with a control diet (CD) devoid of the "active" components. Each diet was consumed during 4 wk with a 4 wk washout period. AD included the following functional concepts: low glycemic impact meals, antioxidant-rich foods, oily fish as source of long-chain omega-3 fatty acids, viscous dietary fibers, soybean and whole barley kernel products, almonds, stanols and a probiotic strain (Lactobacillus plantarum Heal19/DSM15313). Results: Although the aim was to improve metabolic markers without promoting body weight loss, minor weight reductions were observed with both diets (0.9-1.8 +/- 0.2%; P < 0.05). CD did not modify the metabolic variables measured. AD promoted significant changes in total serum cholesterol (-26 +/- 1% vs baseline; P < 0.0001), LDL-cholesterol (-34 +/- 1%; P < 0.0001), triglycerides (-19 +/- 3%; P = 0.0056), LDL/HDL (-27 +/- 2%; P < 0.0001), apoB/apoA1 (-10 +/- 2%; P < 0.0001), HbA1c (-2 +/- 0.4%; P = 0.0013), hs-CRP (-29 +/- 9%; P = 0.0497) and systolic blood pressure (-8 +/- 1%, P = 0.0123). The differences remained significant after adjustment for weight change. After AD, the Framingham cardiovascular risk estimate was 30 +/- 4% (P < 0.0001) lower and the Reynolds cardiovascular risk score, which considers CRP values, decreased by 35 +/- 3% (P < 0.0001). Conclusion: The improved biomarker levels recorded in healthy individuals following the multifunctional regime suggest preventive potential of this dietary approach against CMD

Oat Polar Lipids Improve Cardiometabolic-Related Markers after Breakfast and a Subsequent Standardized Lunch : A Randomized Crossover Study in Healthy Young Adults

It has been suggested that intake of polar lipids may beneficially modulate various metabolic variables. The purpose of this study was to evaluate the effect of oat polar lipids on postprandial and second meal glycemic regulation, blood lipids, gastrointestinal hormones, and subjective appetite-related variables in healthy humans. In a randomized design, twenty healthy subjects ingested four liquid cereal-based test beverages (42 g of available carbohydrates) containing: i. 30 g of oat oil with a low concentration (4%) of polar lipids (PLL), ii. 30 g of oat oil containing a high concentration (40%) of polar lipids (PLH), iii. 30 g of rapeseed oil (RSO), and iv. no added lipids (NL). The products were served as breakfast meals followed by a standardized lunch. Test variables were measured at fasting and during 3 h after breakfast and two additional hours following a standardized lunch. PLH reduced glucose and insulin responses after breakfast (0-120 min) compared to RSO, and after lunch (210-330 min) compared to RSO and PLL (p < 0.05). Compared to RSO, PLH resulted in increased concentrations of the gut hormones GLP-1 and PYY after the standardized lunch (p < 0.05). The results suggest that oat polar lipids have potential nutraceutical properties by modulating acute and second meal postprandial metabolic responses

Relationship Between Microstructure and in Vitro Digestibility of Starch in Precooked Leguminous Seed Flours

Precooked flours (PCFs) were prepared by milling boiled and freeze-dried red kidney beans, white beans and lentils. As demonstrated by scanning electron microscopy, PCFs were rich in relatively large particles which contained cell structures filled with starch. In contrast, flours from raw seeds contained a large number of free starch granules. The in vitro a-amylolysis rate ofPCFs was remarkably low, but increased after physical and chemical treatments of the flours. Homogenization resulted in the largest increase of hydrolysis rate. The susceptibility to enzymatic hydrolysis was also enhanced when PCFs were preincubated with pepsin or submitted to additional boiling. These treatments promoted evident alterations in the microscopic appearance of the cotyledon cell walls of the PCFs, changes that ranged from an apparently thinner surface (pepsin effect), to an almost complete disruption (homogenization effect). A flour prepared from boiled and vacuum-dried red beans showed Jess structural integrity and greater rate of amylolysis than the corresponding PCF, indicating that the drying procedure may influence the microstructural and digestibility features of precooked legumi nous materials. Neither cell walls nor starch granules were observed after suspending PCFs in 2N KOH, giving support to the use of alkaline pre-treatment for the evaluation of to tal starch content of PCFs by enzymatic procedures. The present results suggest the persistence of starch granules enclosed in cotyledon cells as a primary reason for the limited enzymatic availability of starch in PCFs

A randomized trial involving a multifunctional diet reveals systematic lipid remodeling and improvements in cardiometabolic risk factors in middle aged to aged adults

BackgroundA multifunctional diet (MFD) combining foods and ingredients with proven functional properties, such as fatty fish and fiber-rich foods, among others, was developed and shown to markedly reduce cardiometabolic risk-associated factors.ObjectiveHere, we aim at examining metabolic physiological changes associated with these improvements.MethodsAdult overweight individuals without other risk factors were enrolled in an 8-week randomized controlled intervention following a parallel design, with one group (n = 23) following MFD and one group (n = 24) adhering to a control diet (CD) that followed the caloric formula (E%) advised by the Nordic Nutritional Recommendations. Plasma metabolites and lipids were profiled by gas chromatography and ultrahigh performance liquid chromatography/mass spectrometry.ResultsWeight loss was similar between groups. The MFD and CD resulted in altered levels of 137 and 78 metabolites, respectively. Out of these, 83 were uniquely altered by the MFD and only 24 by the CD. The MFD-elicited alterations in lipid levels depended on carbon number and degree of unsaturation.ConclusionAn MFD elicits weight loss-independent systematic lipid remodeling, promoting increased circulating levels of long and highly unsaturated lipids.Clinical trial registrationhttps://clinicaltrials.gov/ct2/show/NCT02148653?term=NCT02148653&amp;draw=2&amp;rank=1, NCT02148653

Update of the concept of type 5 resistant starch (RS5) : Self-assembled starch V-type complexes

Background: All starch fractions not digested and absorbed in the small intestine of healthy humans are considered resistant starch (RS), and their habitual intake has been associated with different beneficial effects on health. In particular, starch-lipid V-type complexes, such as starch-fatty acids and starch-monoglycerides, have traditionally been classified as type 5 RS. Scope and approach: Other starch V-type complexes have emerged more recently, such as starch-glycerol, starch-amino acids, starch-peptides, starch-proteins, starch-lipid-protein, starch-polyphenols, starch-other polysaccharides, among others, which do not fit well into the traditionally accepted nutritional classification of RS. Here, these complexes are analyzed in order to highlight the pertinence of redefining RS5 for the inclusion of this new type of RS. Key findings and conclusions: All those self-assembled starch V-type complexes can be classified as RS5. However, starch-polyphenol V-type complexes should particularly be further investigated to ensure their inclusion in this new extended RS5

A multifunctional diet improves cardiometabolic-related biomarkers independently of weight changes: an 8-week randomized controlled intervention in healthy overweight and obese subjects.

A multifunctional diet (MFD) was previously shown to reduce blood lipids, CRP and blood pressure in a 4-week intervention under weight-maintenance conditions. Here, MFD effects were evaluated in an 8-week intervention with no restriction for weight changes

Effect of Processing on Blood Glucose and Insulin Responses to Starch in Legumes

Postprandial glycemic and insulinemic responses to variously processed red kidney beans were evaluated in normal subjects. The dried seeds were (a) boiled; (b) autoclaved; (c) boiled, freeze-dried, and milled to obtain a precooked flour (PCF) rich in cell-enclosed starch; or (d) milled, steam-cooked, and freezedried to yield a flour containing free starch (FSF). All bean products elicited lower metabolic responses than white wheat bread, used as a reference. Judged from the glycemic and insulinemic indices and the postprandial peak concentrations, autoclaved beans, PCF, and FSF had a more “rapid” behavior than the boiled seeds. Responses to autoclaved beans and PCF were intermediate between boiled beans and the FSF. The insulinemic indices of a lentil PCF and white bread were similar, indicating that different legumes may have different susceptibilities to processing. It is concluded that both the cellular and the cotyledon tissue structures are important determinants of the metabolic responses to legumes

Starch digestibility in the diabetic rat

The digestibility of a mixture of starches was evaluated in balance experiments, with both normal and streptozotocin-induced diabetic rats treated with antibiotics to prevent colonic fermentation of unabsorbed material. Pancreatic amylase production in the diabetic animals was only 10% of the normal level. In spite of this, only a minor decrease in the total starch digestibility index was recorded for the diabetic group (91% vs 94% in control animals). Although small, such a decreased starch digestibility might aggravate gastrointestinal problems in diabetics and should be acknowledged