6 research outputs found
Oxidative stress and autologous IgG binding to band 3 dimers in erythrocytes of diabetic subjects
Fractal analysis of monocytes in diabetes
Fractal dimension ofpericellular membraneof monocytes was evaluated in diabetic patients and in control subjects.
Monocytes were collected from normal healthy volunteers (n= 6) and from diabetic (type 1 and type 2) patients (n= 9).
Monocytes from healthy volunteers were also stimulated in vitro with the ionophore A23187 or with the oligopeptide FMLP.
Monocytes,obtainedbyFicoll-Hypaque,wereexaminedwithaPhilips300transmissionelectronmicroscope.Thecellcontour
was extracted, resized to a standard dimension and converted to a single pixel outline. Box-counting method was then applied
to determine the fractal dimension. Fractal dimensions of monocytes appeared statistically increased in diabetic patients (type1 and type 2), compared with sex- and age-matched controls (p<0.01, p<0.01). The mechanism underlying the observed increased complexity of pericellular membrane may be explained by the in vivo activation of the circulating monocyte in
diabetes. In effect, fractal analysis of stimulated in vitro monocytes showed a significant increase of complexity of pericellular membrane, compared with their controls (p<0.001). Our approach was able to assess and quantitatively evaluate in diabetic patients morphological modifications of the monocyte linked to its activation, offering new parameters useful to follow the effects of therapeutical procedure