Hydrolysis of 5-Chloro-7-iodo-8-quinolinol Conjugates catalyzed by Metal Ions

Glucuronide (CF-G) and sulfate (CF-S) of 5-chloro-7-iodo-8-quinolinol were hydrolyzed in water in the presence of metal ions at pH 7. In this condition, CF-G was hydrolyzed more easily than CF-S. The rate of the hydrolysis, pseudo-first order reaction, was in parallel with the stability constants of 8-quinolinol-metal complexes. The rate of hydrolysis increased with reduction of concentration of H+ and tris buffer suppressed the hydrolysis of CF-G. These results suggest that metal catalyzed hydrolysis of CF-G and CF-S was derived from the formation of complexes between the conjugates and metal ions

Analysis of 5-Chloro-7-iodo-8-quinolinol Conjugates by High Performance Liquid Chromatography

An analytical method of 5-chloro-7-iodo-8-quinolinol (Clioquinol or Chinoform) (CF) conjugates was established by high performance liquid chromatography. The chromatographic conditions were as follows : column, SAX (50 cm×2 mm i.d.) ; mobile phase, I (0.02M borate buffer-0.1M KCl, pH9.5), II (0.02M borate buffer-0.5M NaClO4, pH 9.5) ; gradient, 0→25% (II/I+II) at 3%/min ; column pressure, 500 psi ; flow rate, 1 ml/min ; detector, ultraviolet-detector at 254 mμ. Urine samples of a man administered CF were injected directly to the column, and the major metabolite, CF glucuronide was determined, while very small amounts of CF sulfate was detected

A Superoxide Dismutase-like Substance in Rabbit Plasma

Superoxide dismutase (SOD)-like activity was found in rabbit plasma and the active substance was separated. Its properties were similar to those of copper-zinc SOD in cyanide and azide sensitivity and heat-stability, but its molecular weight was presumed to be 140000 by Sephadex G-150 column