368 research outputs found
In Vivo Tracking of Transplanted Mononuclear Cells Using Manganese-Enhanced Magnetic Resonance Imaging (MEMRI)
BACKGROUND: Transplantation of mononuclear cells (MNCs) has previously been tested as a method to induce therapeutic angiogenesis to treat limb ischemia in clinical trials. Non-invasive high resolution imaging is required to track the cells and evaluate clinical relevance after cell transplantation. The hypothesis that MRI can provide in vivo detection and long-term observation of MNCs labeled with manganese contrast-agent was investigated in ischemic rat legs. METHODS AND FINDINGS: The Mn-labeled MNCs were evaluated using 7-tesla high-field magnetic resonance imaging (MRI). Intramuscular transplanted Mn-labeled MNCs were visualized with MRI for at least 7 and up to 21 days after transplantation in the ischemic leg. The distribution of Mn-labeled MNCs was similar to that of ¹¹¹In-labeled MNCs measured with single-photon emission computed tomography (SPECT) and DiI-dyed MNCs with fluorescence microscopy. In addition, at 1-2 days after transplantation the volume of the site injected with intact Mn-labeled MNCs was significantly larger than that injected with dead MNCs, although the dead Mn-labeled MNCs were also found for approximately 2 weeks in the ischemic legs. The area covered by CD31-positive cells (as a marker of capillary endothelial cells) in the intact Mn-MNCs implanted site at 43 days was significantly larger than that at a site implanted with dead Mn-MNCs. CONCLUSIONS: The present Mn-enhanced MRI method enabled visualization of the transplanted area with a 150-175 µm in-plane spatial resolution and allowed the migration of labeled-MNCs to be observed for long periods in the same subject. After further optimization, MRI-based Mn-enhanced cell-tracking could be a useful technique for evaluation of cell therapy both in research and clinical applications
Radiolytic Decomposition of the structurally similar 11C labelled PET radiopharmaceuticals for benzodiazepine receptors
15th International Symposium on Radiopharmaceutical Chemistr
Synthesis of 4-Amino-N-11CH3-phenylalanine Via Reductive Amination
5th International Peptide Symposiu
Involvement of organic anion transporter 3 in the efflux of 99mTc-L,L-ECD metabolite from mice brain
the 19th International Symposium on Radiopharmaceutical Science
Stability of 11C-radiopharmaceuticals against radiolysis and their synthesis at high radiochemical purity
第6回Nuclear and Radiochemistry カンファレンスにおいて上記のタイトルの発表を行った。また同時にドイツ ユーリッヒ研究所のスタッフおよびハンガリー ATOMKIのスタッフとそれぞれ放射性ヨウ素および励起関数測定に関する意見交換を行った。Sixth Conference on Niclear and Radiochemistr
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