Reduced expression of NFAT-associated genes in UCB versus adult CD4plus T lymphocytes during primary stimulation

The cellular and molecular mechanisms underlying the blunted allo-responsiveness of umbilical cord blood (UCB) T cells have not been fully elucidated. Protein expression of NFATc2 (nuclear factor of activated T cells c2), a critical transcription factor necessary for up-regulation of multiple cytokines known to amplify T-cell allogeneic responses, is reduced in UCB T cells. Affymetrix oligonucleotide microarrays were used to compare gene expression of primary purified CD4+ UCB T cells to adult peripheral blood CD4+ T cells (AB) at baseline, 6, and 16 hours of primary stimulation. NFAT-regulated genes exhibited lower expression in UCB CD4+ T cells including the following: granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon-{gamma} (IFN-{gamma}), tumor necrosis factor-{alpha} (TNF-{alpha}), interleukin 3 (IL-3), IL-4, IL-5, IL-13, IL-2 receptor {alpha} (IL-2R{alpha}; CD25), CD40L, and macrophage inflammatory protein 1 {alpha} (MIP-1{alpha}). Transcription factors involved in the NFAT pathway including C/EBP{beta}, JunB, and Fosl1 (Fra-1), as well as Th1- and Th2-related transcription factors STAT4 (signal transducers and activators of transcription 4), T-bet, and c-maf showed reduced expression in UCB compared with AB during primary stimulation. Reduced cytokine, chemokine, and receptor expression was also found in UCB. Gene array data were confirmed using RNase protection assays, flow cytometry, and quantitative multiplexed cytokine measurements. Reduced global expression of NFAT-associated genes, as well as cytokines and chemokines, in UCB CD4+ T cells may contribute to the decreased graft-versus-host disease (GVHD) observed after UCB transplantation

Measuring revisions to subjective expectations

This paper develops a new metric to measure revisions to subjective expectations and proposes a survey design strategy that enables the estimation of the metric. As an application, I analyze how women update their ex pectations about the effectiveness of contraception methods. The women interviewed exhibit substantial heterogeneity in the way they revise their expectations with receipt of the same information. When relating the heterogeneity in the updating process to observable characteristics, I find that schooling, having a regular sexual partner and knowledge and use of birth control methods have a large impact on the revision process about the effectiveness of contraceptives. © 2007 Springer Science+Business Media, LLC