Phylogenetic tree_JEV E region

Phylogenetic profile showing the relationships between JEV/Bo/Aichi/1/2010 and other JEV isolates based on a comparison of the E gene

Alignment_JEV full genome

Aligned nucleotide sequences of the complete genome of JEV/Bo/Aichi/1/2010 and other JEV isolates (aligned by using MEGA5)

Phylogenetic tree_JEV full genome

Phylogenetic profile showing the relationships between JEV/Bo/Aichi/1/2010 and other JEV isolates based on a comparison of the complete genome

Alignment_JEV E region

Aligned nucleotide sequences of the E gene of JEV/Bo/Aichi/1/2010 and other JEV isolates (aligned by using MEGA5)

Production of microRNA deficient mice with TALEN.

<p>(A) Schematic representation of TALENs that target microRNA. Wild type genomic sequence is shown with black line. miRNA-5p and -3p on the genomic sequence are with orange boxes. TALENs are indicated with green boxes and recognition sequences are indicated with light green boxes. Scissors show the location of cutting site of FokI endonuclease. Genomic sequence of mutated allele is indicated with black line at the bottom. Deleted sequence is shown with a dark orange box with a white waved line. (B) PCR genotyping of the miRNA mutants. Genomic sequence is shown at the top with the target sequences of a pair of TALEN colored in green. Blue lines and boxes indicate pre-miRNAs and mature-miRNAs sequences, respectively. Deleted and inserted nucleotides are indicated with red dashes and blue letters, respectively. The sizes of deletions and insertions are shown to the right of mutated alleles with ▵ and +, respectively. Alleles without mutation are indicated with wt (wild type). (C) Genotyping of F1 offspring of <i>mmu-mir-10a</i> KO (Mutant3) mouse by T7EI assay. Arrows indicate the digested PCR products containing mutation. DNA size marker is shown at left. Lane 1-9: F1 individuals; lane 10: founder; lane 11: Wt. (D) Genotyping of F1 offspring of <i>mmu-mir-10b</i> KO (Mutant1) mouse by T7EI assay. Arrows indicate the digested PCR products containing mutation. DNA size marker is shown at left. Lane 1-6: F1 individuals; lane 7: founder; lane 8: Wt.</p

Cell based assay of TALEN activities. Each sequence shows the result of PCR direct sequencing of cloned PCR products amplified from TALEN transfected cells.

<p>Green letters designate target sequences of TALENs. Red dashes and blue letters indicate deleted and inserted nucleotides, respectively. The sizes of deletions are shown to the right of mutated alleles with ▵ and/or +. Alleles without mutation are indicated with Wild type. The numbers in the parenthesis indicate the number of clones obtained.</p

Gene targeting efficiencies.

*<p>TALENs for <i>mmu-mir-10a</i> and <i>mmu-mir-10b</i> were mixed and microinjected. †Genotype of <i>mmu-mir-10a</i> and <i>mmu-mir-10b</i> were assayed and results were indicated separately in a cell.</p

Real-time RT-PCR analysis of miR-10a*.

<p>Genotype of each animal is indicated at bottom. Mut indicates mutant allele. Expression level of the wt animal was adjusted as 1.</p

PCR genotyping of the gene desert on chromosome 11 mutants.

<p>Genomic sequence is shown at the top with the target sequences of a pair of TALEN colored in green. Nucleotide sequences of mutated alleles are indicated with red dashes. The sizes of deletions are shown to the right of mutated alleles with ▵. Alleles without mutation are indicated with Wild type.</p

Bovine epizootic encephalomyelitis caused by Akabane virus in southern Japan-2

Asm of a neuron (an arrow) and nerve axons (arrow heads). Immunohistochemistry. Bar = 0.1 mm<p><b>Copyright information:</b></p><p>Taken from "Bovine epizootic encephalomyelitis caused by Akabane virus in southern Japan"</p><p>http://www.biomedcentral.com/1746-6148/4/20</p><p>BMC Veterinary Research 2008;4():20-20.</p><p>Published online 13 Jun 2008</p><p>PMCID:PMC2443122.</p><p></p