Ferrocenylnaphthalene Diimide-Based Electrochemical Detection of Aberrant Methylation in hTERT Gene

Since aberrant methylation at CpG sites is linked to the silencing of tumor suppressor genes, DNA methylation analysis is important for cancer diagnosis. We developed ferrocenylnaphthalene diimide (FND), which has two ferrocenyl moieties at the substituent termini, as an electrochemical indicator for hybridized DNA duplexes. In this study, we attempted to detect aberrant methylation of human telomerase reverse transcriptase gene (hTERT), an efficient cancer marker, using FND-based hybridization coupled with electrochemical detection via a multi-electrode chip

Induction of cyclooxygenase-2 in human synovial cells by β2-microglobulin

Induction of cyclooxygenase-2 in human synovial cells by β2-microglobulin.BackgroundProstaglandins (PGs) are important mediators of inflammation in arthritis. We evaluated the role of the cyclooxygenase-2 (COX-2) enzyme, which regulates PG biosynthesis, in osteoarthropathy associated with hemodialysis-associated amyloidosis (HAA) by characterizing COX-2 expression in β2-microglobulin–treated human synovial cells.MethodsWe examined the effects of β2-microglobulin (β2m), a major constituent protein of amyloid fibrils in HAA, on the COX-2 protein and mRNA expression in human synovial cells using Western blot and reverse transcriptase-polymerase chain reaction.Resultsβ2m selectively increased the biosynthesis of COX-2 protein and induction of COX-2 mRNA in a dose-dependent manner. Immunoabsorption of β2m–containing media by anti-β2m–specific antibody abrogated β2m–mediated COX-2 expression on synovial cells. On the other hand, dexamethasone markedly suppressed the induction of COX-2 protein and mRNA in β2m–stimulated synovial cells.ConclusionsOur results suggest that induction of COX-2 expression by β2m may be an important component of the inflammatory process in hemodialysis-associated osteoarthropathy

Optical follow-up observation of Fast Radio Burst 151230

The origin of fast radio bursts (FRBs), bright millisecond radio transients, is still somewhat of a mystery. Several theoretical models expect that the FRB accompanies an optical afterglow (e.g., Totani 2013; Kashiyama et al. 2013). In order to investigate the origin of FRBs, we perform $gri$-band follow-up observations of FRB~151230 (estimated $z \lesssim 0.8$) with Subaru/Hyper Suprime-Cam at $8$, $11$, and $14$~days after discovery. The follow-up observation reaches a $50\%$ completeness magnitude of $26.5$~mag for point sources, which is the deepest optical follow-up of FRBs to date. We find $13$ counterpart candidates with variabilities during the observation. We investigate their properties with multicolor and multi-wavelength observations and archival catalogs. Two candidates are excluded by the non-detection of FRB~151230 in the other radio feed horns that operated simultaneously to the detection, as well as the inconsistency between the photometric redshift and that derived from the dispersion measure of FRB~151230. Eight further candidates are consistent with optical variability seen in AGNs. Two more candidates are well fitted with transient templates (Type IIn supernovae), and the final candidate is poorly fitted with all of our transient templates and is located off-center of an extended source. It can only be reproduced with rapid transients with a faint peak and rapid decline and the probability of chance coincidence is $\sim3.6\%$. We also find that none of our candidates are consistent with Type Ia supernovae, which rules out the association of Type Ia supernovae to FRB~151230 at $z\leq0.6$ and limits the dispersion measure of the host galaxy to $\lesssim300$~pc~cm$^{-3}$ in a Type Ia supernova scenario.Comment: 22 pages, 11 figures. Accepted for publication in PASJ (Publications of the Astronomical Society of Japan