Phenotypic characterization of <i>Synechocystis</i> sp. PCC 6803 substrains reveals differences in sensitivity to abiotic stress - Fig 6

<p>Batch growth of <i>Synechocystis</i> sp. PCC 6803 substrains GT-L (white circles), GT-B (red triangles) and PCC-B (blue squares) in BG11 medium (A) and BG11 medium supplemented with 0.5 M NaCl at the beginning of the batch cultivation (B). The plotted values represent averages of at least three independent experiments, the error bars represent standard errors.</p

Growth rates of <i>Synechocystis</i> sp. PCC 6803 substrains GT-L (white circles), GT-B (red triangles) and PCC-B (blue squares) under increasing red, blue and white light.

<p>A, B–Red light was set to intensities of 25–660 μmol(photons) m^-2 s^-1 and it was supplemented with 25 μmol(photons) m^-2 s^-1 of blue light. The experiments were performed at 32°C (A) and 35°C (B). C–Red light of 220 μmol(photons) m^-2 s^-1 was supplemented with 25–220 μmol(photons) m^-2 s^-1 of blue light under 32°C. D–Red light of 220–8 μmol(photons) m^-2 s^-1 was supplemented with white light of 0–790 μmol(photons) m^-2 s^-1 (at 32°C) in order to keep the red photons (as a combination of red light and part of the white spectra) constant at 220 μmol(photons) m^-2 s^-1 (according to [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0189130#pone.0189130.ref019" target="_blank">19</a>]). All experiments were carried out in a quasi-continuous regime as described in the main text under growth saturating CO₂ concentration of 5 000 ppm. Each point represents average of at least four independent experiments, error bars represent standard errors. The dashed lines in panels A and B represent fitting of the data points by the function derived by Platt et al. (1980) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0189130#pone.0189130.ref051" target="_blank">51</a>]. The dashed lines in panels C and D represent linear fitting of the data points by the least squares method. Legend in panel D represents particular combinations of white light (W) and red light (R) in units of μmol(photons) m^-2 s^-1.g.</p

Phenotypic characterization of <i>Synechocystis</i> sp. PCC 6803 substrains reveals differences in sensitivity to abiotic stress - Fig 4

<p>Cell size (A), dry weight (B), and glycogen content (C) of <i>Synechocystis</i> substrains GT-L (white bars), GT-B (red bars) and PCC-B (blue bars) under 25, 220 and 660 μmol(photons) m^-2 s^-1 of red light complemented with 25 μmol(photons) m^-2 s^-1 of blue light. The cells were cultivated at 32°C under input CO₂ concentration of 5 000 ppm in a quasi-continuous regime as described in the main text. Each value represents average from at least three independent experiments, error bars represent standard errors. Differences in cell size, dry weight and glycogen content among <i>Synechocystis</i> substrains are marked by letters above the particular columns (Tukey’s HSD post-hoc test following one-way ANOVA: p<0.05).</p