Identifikacija i klasifikacija izolata virusa mozaika soje nađenih na Kosovu

Three virus isolates of soybean mosaic virus (SBMV) have been isolated from soybean cultivated in the Province of Kosovo (southeastern part of Yugoslavia). It has been established on the basis of test-plant reactions, serology, virus stability in sap and type of virus particle. The isolates are identical with each other and belong to Gl-strain group of SBMV. It has been found by ELISA-test that the isolates studied occur in the same concentration in endosperm and embryo of soybean seed produced in infected plants.Iz primjeraka soje gajene na Kosovu izdvojena su tri virusna izoalta koji pripadaju virusu mozaika soje (SBMV). Dva su izolata potjecala iz biljaka gajenih u polju (izolati SK i SV), a treći je izolat (SP) nađen u merkantilnom sjemenu soje. Da izolati pripadaju SBMV virusu, utvrđeno je na osnovi reakcija na pokusnim biljkama, seroloških osobina, njihova vladanja in vitro te na osnovi tipa virusnih čestica. Nađeni su izolati međusobno identični i najvjerojatnije pripadaju Gl-skupini sojeva SBMV-a. S pomoću testa ELISA utvrđeno je da studiram izolati dolaze u istim koncentracijama u endospermu i embriju sjemenaka inficiranih biljaka

Research Notes : United States : Genetics of reaction to soybean mosaic virus (SMV) in the cultivars \u27Kawnggyo\u27, \u27Marshall\u27, and PI 96983

Several genes conditioning resistance to SMV have been found and some have been assigned gene symbols. In addition, a series of SMV strain groups has been differentiated by their interactions with a selected group of culti-vars (Cho and Goodman, 1979; Lim, 1985). We have undertaken a study of the genes conditioning the reactions of certain differential cultivars to SMV in an attempt to establish their relationships with symbolized genes

Research Notes : United States : Genetics of reaction to soybean mosaic virus (SMV) in cultivars exhibiting differential reaction to SMV strains.

A series of seven differential strain groups of SMV, labelled Gl to G7, were reported by Cho and Goodman (1979). Buzzell and Tu (1984) added another strain, G7A, and Lim (1985) added an additional strain, Cl4. We have been studying the genetic relationships among the cultivars that define the strain groups. Our intial studies involved the cultivars \u27Marshall\u27, \u27Kwanggyo\u27, \u27York\u27, \u27Ogden\u27, and PI 96.983, which differentiate the seven strain groups of Cho and Goodman

Identifikacija i klasifikacija izolata virusa mozaika soje nađenih na Kosovu

Three virus isolates of soybean mosaic virus (SBMV) have been isolated from soybean cultivated in the Province of Kosovo (southeastern part of Yugoslavia). It has been established on the basis of test-plant reactions, serology, virus stability in sap and type of virus particle. The isolates are identical with each other and belong to Gl-strain group of SBMV. It has been found by ELISA-test that the isolates studied occur in the same concentration in endosperm and embryo of soybean seed produced in infected plants.Iz primjeraka soje gajene na Kosovu izdvojena su tri virusna izoalta koji pripadaju virusu mozaika soje (SBMV). Dva su izolata potjecala iz biljaka gajenih u polju (izolati SK i SV), a treći je izolat (SP) nađen u merkantilnom sjemenu soje. Da izolati pripadaju SBMV virusu, utvrđeno je na osnovi reakcija na pokusnim biljkama, seroloških osobina, njihova vladanja in vitro te na osnovi tipa virusnih čestica. Nađeni su izolati međusobno identični i najvjerojatnije pripadaju Gl-skupini sojeva SBMV-a. S pomoću testa ELISA utvrđeno je da studiram izolati dolaze u istim koncentracijama u endospermu i embriju sjemenaka inficiranih biljaka

A Transcriptional Regulatory Network of \u3cem\u3eRsv3\u3c/em\u3e-Mediated Extreme Resistance against \u3cem\u3eSoybean Mosaic Virus\u3c/em\u3e

Resistance genes are an effective means for disease control in plants. They predominantly function by inducing a hypersensitive reaction, which results in localized cell death restricting pathogen spread. Some resistance genes elicit an atypical response, termed extreme resistance, where resistance is not associated with a hypersensitive reaction and its standard defense responses. Unlike hypersensitive reaction, the molecular regulatory mechanism(s) underlying extreme resistance is largely unexplored. One of the few known, naturally occurring, instances of extreme resistance is resistance derived from the soybean Rsv3 gene, which confers resistance against the most virulent Soybean mosaic virus strains. To discern the regulatory mechanism underlying Rsv3-mediated extreme resistance, we generated a gene regulatory network using transcriptomic data from time course comparisons of Soybean mosaic virus-G7-inoculated resistant (L29, Rsv3-genotype) and susceptible (Williams82, rsv3-genotype) soybean cultivars. Our results show Rsv3 begins mounting a defense by 6 hpi via a complex phytohormone network, where abscisic acid, cytokinin, jasmonic acid, and salicylic acid pathways are suppressed. We identified putative regulatory interactions between transcription factors and genes in phytohormone regulatory pathways, which is consistent with the demonstrated involvement of these pathways in Rsv3-mediated resistance. One such transcription factor identified as a putative transcriptional regulator was MYC2 encoded by Glyma.07G051500. Known as a master regulator of abscisic acid and jasmonic acid signaling, MYC2 specifically recognizes the G-box motif (“CACGTG”), which was significantly enriched in our data among differentially expressed genes implicated in abscisic acid- and jasmonic acid-related activities. This suggests an important role for Glyma.07G051500 in abscisic acid- and jasmonic acid-derived defense signaling in Rsv3. Resultantly, the findings from our network offer insights into genes and biological pathways underlying the molecular defense mechanism of Rsv3-mediated extreme resistance against Soybean mosaic virus. The computational pipeline used to reconstruct the gene regulatory network in this study is freely available at https://github.com/LiLabAtVT/rsv3-network

A transcriptional regulatory network of Rsv3-mediated extreme resistance against Soybean mosaic virus.

Resistance genes are an effective means for disease control in plants. They predominantly function by inducing a hypersensitive reaction, which results in localized cell death restricting pathogen spread. Some resistance genes elicit an atypical response, termed extreme resistance, where resistance is not associated with a hypersensitive reaction and its standard defense responses. Unlike hypersensitive reaction, the molecular regulatory mechanism(s) underlying extreme resistance is largely unexplored. One of the few known, naturally occurring, instances of extreme resistance is resistance derived from the soybean Rsv3 gene, which confers resistance against the most virulent Soybean mosaic virus strains. To discern the regulatory mechanism underlying Rsv3-mediated extreme resistance, we generated a gene regulatory network using transcriptomic data from time course comparisons of Soybean mosaic virus-G7-inoculated resistant (L29, Rsv3-genotype) and susceptible (Williams82, rsv3-genotype) soybean cultivars. Our results show Rsv3 begins mounting a defense by 6 hpi via a complex phytohormone network, where abscisic acid, cytokinin, jasmonic acid, and salicylic acid pathways are suppressed. We identified putative regulatory interactions between transcription factors and genes in phytohormone regulatory pathways, which is consistent with the demonstrated involvement of these pathways in Rsv3-mediated resistance. One such transcription factor identified as a putative transcriptional regulator was MYC2 encoded by Glyma.07G051500. Known as a master regulator of abscisic acid and jasmonic acid signaling, MYC2 specifically recognizes the G-box motif ("CACGTG"), which was significantly enriched in our data among differentially expressed genes implicated in abscisic acid- and jasmonic acid-related activities. This suggests an important role for Glyma.07G051500 in abscisic acid- and jasmonic acid-derived defense signaling in Rsv3. Resultantly, the findings from our network offer insights into genes and biological pathways underlying the molecular defense mechanism of Rsv3-mediated extreme resistance against Soybean mosaic virus. The computational pipeline used to reconstruct the gene regulatory network in this study is freely available at https://github.com/LiLabAtVT/rsv3-network