11 research outputs found

    How Microbiome Composition Correlates with Biochemical Changes during Sauerkraut Fermentation: a Focus on Neglected Bacterial Players and Functionalities

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    This study provided a new perspective on the bacterial community succession during sauerkraut fermentation, and on resulting metabolic functions. While culture-dependent methods confirmed the key role of the well-known core microbiome species, metagenomic approach (shotgun) revealed Secundilactobacillus malefermentans as a species of the core microbiome, especially during the last weeks of fermentation. Although the potentiality of S. malefermentans has not yet fully explored, it held core functional genes usually attributed to others lactic acid bacteria driving sauerkraut fermentation. Based on our results it is arguable that S. malefermentans might have a key a role during sauerkraut fermentation carried out at low temperature. Under our experimental conditions, the profile of phenolic compounds changed throughout sauerkraut fermentation. The amount of free phenolics, including free phenolic acids, increased at the beginning of the fermentation, whereas the conversion of phenolic acids into microbial derivatives was consistent during the last part of the sauerkraut fermentation. We pioneered correlating changes in the phenolics profile to changes in the microbiome, although the framework presented is still fragmentary. Annotated genes linked to the phenolic compounds metabolism (VprA and padA) were found in many core species during the whole process. A high metabolic potential for phenolics bioconversion emerged for lactobacilli and Pediococcus spp. through correlation analysis between microbiome composition and phenolics profile

    Comparative study between supported bimetallic catalysts for nitrate remediation in water

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    As the population grows and the demand for water rises, the development of efficient and sustainable water purification techniques is becoming increasingly important to ensure access to clean and safe water in the future. The pollution of surface and groundwater by nitrate ( NO 3 - {\text{NO}}_{3}{-} ) is a growing global concern due to the rise in nitrogen-rich waste released from agriculture and industry. The removal of nitrate ions from aqueous media using bimetallic catalysts loaded on several supports was studied. Multiwalled carbon nanotubes, activated carbon, titanium dioxide, titanium dioxide/multiwalled carbon nanotubes, and Santa Barbara Amorphous-15 were used as supports to synthesize these bimetallic catalysts. The effects of the support type, supported metal, and catalyst reduction method on the nitrate reduction activity in water were investigated. The catalysts were characterized by X-ray diffraction, fourier-transform infrared spectroscopy, Brunauer-Emmett-Teller isotherm, inductively coupled plasma spectroscopy, and field emission gun scanning transmission electron microscope. In terms of nitrate conversion, high-temperature hydrogen reduction of the catalysts was a more effective method of catalyst preparation than NaBH4 reduction. Except for the carbon nanotube-TiO2 composite, pH fixation using CO2 flow improved the efficiency of supported catalysts. The catalysts 1Pd-1Cu/TiO2 and 1Pd-Cu/SBA-15 presented the highest catalytic activity, but the latter was the most selective to nitrogen

    Synthesis of 5,6- and 6,6-Spirocyclic Compounds

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    Cycloisomerization of π‐Coupled Heteroatom Nucleophiles by Gold Catalysis: En Route to Regiochemically defined Heterocycles

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