Management of hospital wastes

Management of hospital waste

Use of >100,000 NHLBI Trans-Omics for Precision Medicine (TOPMed) Consortium whole genome sequences improves imputation quality and detection of rare variant associations in admixed African and Hispanic/Latino populations

Most genome-wide association and fine-mapping studies to date have been conducted in individuals of European descent, and genetic studies of populations of Hispanic/Latino and African ancestry are limited. In addition, these populations have more complex linkage disequilibrium structure. In order to better define the genetic architecture of these understudied populations, we leveraged >100,000 phased sequences available from deep-coverage whole genome sequencing through the multi-ethnic NHLBI Trans-Omics for Precision Medicine (TOPMed) program to impute genotypes into admixed African and Hispanic/Latino samples with genome-wide genotyping array data. We demonstrated that using TOPMed sequencing data as the imputation reference panel improves genotype imputation quality in these populations, which subsequently enhanced gene-mapping power for complex traits. For rare variants with minor allele frequency (MAF) 86%. Subsequent association analyses of TOPMed reference panel-imputed genotype data with hematological traits (hemoglobin (HGB), hematocrit (HCT), and white blood cell count (WBC)) in ~21,600 African-ancestry and ~21,700 Hispanic/Latino individuals identified associations with two rare variants in the HBB gene (rs33930165 with higher WBC [p = 8.8x10-15] in African populations, rs11549407 with lower HGB [p = 1.5x10-12] and HCT [p = 8.8x10-10] in Hispanics/Latinos). By comparison, neither variant would have been genome-wide significant if either 1000 Genomes Project Phase 3 or Haplotype Reference Consortium reference panels had been used for imputation. Our findings highlight the utility of the TOPMed imputation reference panel for identification of novel rare variant associations not previously detected in similarly sized genome-wide studies of under-represented African and Hispanic/Latino populations

Mendelian randomization supports bidirectional causality between telomere length and clonal hematopoiesis of indeterminate potential

Human genetic studies support an inverse causal relationship between leukocyte telomere length (LTL) and coronary artery disease (CAD), but directionally mixed effects for LTL and diverse malignancies. Clonal hematopoiesis of indeterminate potential (CHIP), characterized by expansion of hematopoietic cells bearing leukemogenic mutations, predisposes both hematologic malignancy and CAD. TERT (which encodes telomerase reverse transcriptase) is the most significantly associated germline locus for CHIP in genome-wide association studies. Here, we investigated the relationship between CHIP, LTL, and CAD in the Trans-Omics for Precision Medicine (TOPMed) program (n = 63,302) and UK Biobank (n = 47,080). Bidirectional Mendelian randomization studies were consistent with longer genetically imputed LTL increasing propensity to develop CHIP, but CHIP then, in turn, hastens to shorten measured LTL (mLTL). We also demonstrated evidence of modest mediation between CHIP and CAD by mLTL. Our data promote an understanding of potential causal relationships across CHIP and LTL toward prevention of CAD

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Not AvailableCynodon dactylon Pers. is highly valued warm season turf grass having global adaptability, robustness and resistance to trampling. In the present study, the ISSR protocol was standardized and quantities of template DNA, dNTPs, MgCl2, Taq DNA polymerase, primer concentration and annealing temperature for each primer were worked out. The reproducible amplifiable products were obtained in all PCR reactions. Analysis of molecular variance (AMOVA), Genetic diversity, Nei’s gene diversity, Shannon’s index, and Nei’s unbiased genetic distance, partition, within- and among-group, of all parameters was analyzed. Levels of genetic divergence between samples were calculated with the fixation index PhiPT. Statistics with AMOVA revealed 1 and 99 % variance among and within various mutants, respectively. Cluster analysis based on the Unweighted-Pair Group Method arithmetic Average (UPGMA), principal coordinate analysis (PCA) and Spatial correlation is a measured that looks at the relationship (genetic distance) amongst mutants. PCOA analysis of ISSR data showed that the first three factors comprised about 75.20% of total variance when the first, second and third axis comprised about 36.64, 23.96 and 14.63% of total variance, respectively. Variation within mutants was the maximum in DFR-C-448 followed by DFR-C-446(10.357). In DFR-C-448 unique number of bands to a single population was observed. Correlogram plot shows that there is significant positive genetic structure at distance class of mutants DFR-C-448Not Availabl

Time and frequency domain connectedness and spill-over among fintech, green bonds and cryptocurrencies in the age of the fourth industrial revolution

The study in the age of the 4th industrial revolution examines the time and frequency domain connectedness and spill-over among Fintech, green bonds, and cryptocurrencies. Using daily data from November 2018 to June 2020, we use both DY (Diebold & Yilmaz, 2012) and BK (Baruník et al., 2017) to examine the volatility connectedness of returns series. The results of DY suggest that, first, the total connectedness of 21st century technology assets and traditional common stocks is very high, and hence in the turbulent economy, there is a high probability of contemporaneous losses. Second, Bitcoin, MSCIW, MSCI US, and KFTX are net contributors of volatility shocks whereas US dollar, oil, gold, VIX, green bond and green bond select are net receivers. Therefore, Fintech and common equities are not good hedging instruments in the same portfolio. Third, the short-term witnesses higher volatility transmission than the long-term. That is, holding assets for a long-term is likely to mitigate risks whereas trading financial assets in the short-term can increase risk because of higher volatility. Fourth, the traditional assets, gold and oil, as well as modern assets, green bonds, are useful as good hedgers compared with other assets because shock transmissions from them to Fintech, KFTX are below 0.1% and, more importantly, the total volatility spill-over of all assets in the sample is moderately average, accounting for 44.39%

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Not AvailableIn this study, leaves sample of eight turf grass genera were used to standardized the ISSR protocol and quantities of template DNA, dNTPs, MgCl2, Taq DNA polymerase, primer concentration and annealing temperature for each primer. Analysis of molecular variance (AMOVA), Genetic diversity, Nei’s gene diversity, Shannon’s index, and Nei’s unbiased genetic distance, partition, within- and among-group, of all parameters was analyzed. Levels of genetic divergence between samples were calculated with the fixation index PhiPT. Statistics with AMOVA revealed 84 and 16 % variance among and within various mutants, respectively. Cluster analysis based on the Unweighted-pair group method arithmetic average (UPGMA), Principal coordinate analysis (PCA) and Spatial correlation is a measured that looks at the relationship (genetic distance) amongst mutants. PCOA analysis of ISSR data showed that the first three factors comprised about 94.5% of total variance when the first, second and third axis comprised about 49.85, 29.29 and 15.36% of total variance, respectively. Statistically non-significant genetic similarity exists among population which varies from -1.0000 to 0.2419. Maximum similarity was recorded between the two samples of Lolium perenne and Agrostis stolonifera (0.0682) followed by Stenotaphrum secundatum, Poa pratensis (0.0419). Cluster analysis was conducted to generate a dendogram elucidating for relationships among turf grass. The first cluster divided into three sub-clusters comprising: (i) Poa pratensis and Agrostis stolonifera (ii) DFR-NS-1 and Lolium perenne and (iii) the DFR-NS-1 Festuca arundinacea.The second cluster consisted of Stenotaphrum secundatum, Zyosia junsia and Paspalum vaginatum. Within the second cluster Paspalum vaginatum was separated from the zoysiagrass. As revealed by ISSR analysis, Stenotaphrum secundatum, and Paspalm were quite distinct from bermudagrass (DFR-NS-1) and rest of the turf grasses.Not Availabl

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Not AvailableCynodon dactylon Pers. is highly valued warm season turf grass having global adaptability, robustness and resistance to trampling. In the present study, the ISSR protocol was standardized and quantities of template DNA, dNTPs, MgCl2, Taq DNA polymerase, primer concentration and annealing temperature for each primer were worked out. The reproducible amplifiable products were obtained in all PCR reactions. Analysis of molecular variance (AMOVA), Genetic diversity, Nei’s gene diversity, Shannon’s index, and Nei’s unbiased genetic distance, partition, within- and among-group, of all parameters was analyzed. Levels of genetic divergence between samples were calculated with the fixation index PhiPT. Statistics with AMOVA revealed 1 and 99 % variance among and within various mutants, respectively. Cluster analysis based on the Unweighted-Pair Group Method arithmetic Average (UPGMA), principal coordinate analysis (PCA) and Spatial correlation is a measured that looks at the relationship (genetic distance) amongst mutants. PCOA analysis of ISSR data showed that the first three factors comprised about 75.20% of total variance when the first, second and third axis comprised about 36.64, 23.96 and 14.63% of total variance, respectively. Variation within mutants was the maximum in DFR-C-448 followed by DFR-C-446(10.357). In DFR-C-448 unique number of bands to a single population was observed. Correlogram plot shows that there is significant positive genetic structure at distance class of mutants DFR-C-448.Not Availabl

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Not AvailableAn experiment was conducted with 26 gladiolus cultivars at Research Farm of Directorate of Floricultural Research, New Delhi. The minimum days required for sprouting was recorded in cultivar Peter Pears (3.40 days), whereas earliest spike initiation (86.33 days) and first floret opening (100.33 days) were observed in cultivar Hunting Song. Maximum plant height (115.87 cm) and spike length (101.20 cm) were found in cultivar Amsterdam, whereas maximum rachis length (73.39 cm) was recorded in Snow Princess. Maximum number of florets per spike (18.60) and bigger florets size (97.72 mm) were recorded in Snow Princess and Priscilla, respectively. The cultivar Yellow Stone was found with maximum number of corms per plant (2.68), whereas highest average corm weight (71.60 g) and corm size (64.87 mm) were recorded in Forta Rosa, and highest cormels weight (17.50 g) in Argentina. Higher values of phenotypic coefficient of variation (PCV) and genotypic coefficient of variation (GCV) were recorded for cormel weight, i.e. 104.36 and 67.26 and number of cormels per plant, i.e. 99.82 and 71.58, respectively. Maximum heritability was recorded for days to first floret opening (87.23%) followed by rachis length (82.07%) and days to spike initiation (78.26%). Highest values for genetic advance were recorded for plant height (26.35) and spike length (23.36). However, low genetic advance was observed for days to sprouting (0.04) followed by number of corms (0.32) and number of leaves per plant (0.59).Not Availabl