Cryopreservation of sheathed third-stage larvae of Nematodirus spathiger

A technique for the cryopreservation of sheathed third-stage larvae of Nematodirus spathiger is described. It consists of incubating larvae in 5% (v/v) ethylene glycol for 20 min at room temperature, followed by 1 min of incubation in 40% (v/v) ethylene glycol at 0°C. The average recovery of adult worms was 28,5% for two adult sheep infected with these larvae.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201

An improved technique for the cryopreservation of Gaigeria pachyscelis (Sandveld hookworm)

A technique for the cryopreservation of third-stage larvae of Gaigeria pachyscelis is described. It consists of incubating sheathed infective larvae at 37°C in 40% (v/v) ethylene glycol for 7 min, followed by 2 min at 0°C in 82,8% (v/v) cryoprotectant mixture, prior to transferring the larvae to liquid nitrogen. The survival rate obtained with this technique is consistently high: 69,1% as assessed by motility.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201

Cryopreservation of sheathed third-stage larvae of Ancylostoma caninum (hookworm of dogs) and Ancylostoma tubaeforme (hookworm of cats)

Sheathed infective third-stage larvae of Ancylostoma caninum and Ancylostoma tubaeforme were successfully cryopreserved in liquid nitrogen. The survival rates, as assessed by motility, were A. caninum 49,2% and A. tubaeforme 51 ,5% after 30 d of cryopreservation .The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201

Bile enhances the infectivity of third stage larvae of Dictyocaulus filaria

Incubating metacyclic larvae of Dictyocaulus filaria in ovine bile solution prior to per os infection significantly enhanced their infectivity to sheep.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201

Cryopreservation of sheathed third-stage larvae of Gaigeria pachyscelis (Sandveld hookworm)

A technique for the cryopreservation of third stage larvae of Gaigeria pachyscelis is described. It consists of incubating sheathed third-stage larvae in 80% (v/v) ethylene glycol as a cryoprotectant for 30 s at 0°C, prior to transfer into liquid nitrogen. The survival rate, as assessed by motility, was 37,6% after 30 d cryopreservation. A sheep infected percutaneously with 700 live cryopreserved third-stage larvae, harboured 41 adult worms (infectivity rate: 5,8%) when necropsied 78 d later.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201

Cryopreservation of third-stage larvae of Strongylus vulgaris (large strongyle of horses)

A technique for the cryopreservation of third-stage larvae of Strongylus vulgaris is described. Infective larvae of S. vulgaris were exsheathed in a 0,16% sodium hypochlorite solution and then transferred into cryotubes containing 0,09% saline. The samples were stored in the gas phase of liquid nitrogen.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format

Radiolabelled adult Haemonchus contortus obtained from eggs voided in the faeces of a sheep injected with ⁵⁹Fe

The infective larvae of Haemonchus contortus (L3) obtained from an artificially infected sheep injected with 125 µCi of ⁵⁹Fe, incorporated the isotope and retained it in sufficient quantities to be detectable by autoradiography in the adult worms which developed. This is apparently the first report of incorporation of ⁵⁹Fe into adult helminths, of which the L3 originated from an animal that was injected with the isotope.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201

Cryopreservation of sheathed third-stage larvae of Oesophagostomum radiatum (nodular worm of cattle)

Sheathed infective larvae of Oesophagostomum radiatum were successfully cryopreserved by the use of a procedure developed for hookworms. The survival rate, as assessed by motility, was 57,9% after 42 d of cryopreservation.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat X Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format