Evaluation of distribution of the scorpion Mesobuthus Eupeus in Kashan

History and Objectives: Considering the high frequency of the scorpion Mesobuthus eupeus in Iran and the critical significance of its biting, this study was carried out to determine its distribution pattern using rock-rolling and black light trapping methods in Kashan from 2000 to 2001. Materials and Methods: The descriptive strategy of this study was carried out on 29 places in Kashan and with 44 samples using rock-rolling and black light trapping methods in Kashan from 2000 to 2001. Results: Out of 29 locations, 44 sampling were done and a total of 239 scorpions were trapped. In this respect, 227 (95) out of them were trapped using rock-rolling method and 18 samples (5) were collected using black light method. The average rate of sampling was 5.43. The average was 7.56 for rock-rolling method and 1.28 for black light method. Conclusion and Recommendations: It was found out that in mountainous locations, more scorpion is trapped using rock-rolling method and the scorpion Mesobuthus eupeus is mainly lithophilic and psammophilic

Surveying the fractions of Mesobuthus Eupeus in Kashan

Background: Mesobuthus eupeus is the most common type of mesobuthus belongs to the buthidae family. It is reported all over Iran and is a common cause of biting. Identifying its fractions could be helpful to draw better therapeutic approach, thus the present study was conducted in Kashan to determine different fractions of mesobuthus eupeus.Materials and Methods: It was a descriptive study. Having prepared Tricine SDS-PAGE and heated the toyin with a buffer containing mercaptoethanol, 25 microgram, of toxin protein was injected to each plate adjusting the electrophoresis on 80V. Following the electrophoresis, 15 hours later, the produced gel was washed and fixed by methanol. Sigma ultra low MW was used to compare molecular weight of toxin protein.Results: Mesobuthus eupeus' toxin contains 6 protein bands. The highest one had the molecular weight of 26.96 KD whereas the lowest band had the molecular weight of 1KD. It has further proteins with lower molecular weight as compared to sigma ultra low MW.Conclusion: Since mesobuthus eupeus could be found all over Iran, further studies are strongly recommended to determine better antivenom

Study of the Effect of Hemiscorpius Lepturus Venom on Levels of WBC, RBCc and Hematocrit in Rats

Introduction: Hemiscorpius lepturus scorpion present in the southern provinces of the country, especially in Khoozestan stings a lot of people that results in long-standing dangerous side effects and sometimes even mortality. As the study of the effects of this scorpion venom on some laboratory animals can determine its mechanism of action and help us to cure people stung by scorpions, this study has been done to study the effects of Hemiscorpius lepturus venom on levels of WBC, RBC and hematocrit of rats. Methods: An experimental study has been done on 51 rats of the same race with approximate age of 2-3 months, and weight of 200-250 grams. . All the hematological features including WBC (White Blood Cell), RBC (Red Blood Cell) and HT (Hematocrit) were measured prior to venom injection. The same procedures were followed after injection of 1 microlitre of venom. Results before and after injection have been analyzed by Wilcoxon Matched, Pairs signed and Ranks statistical tests. Results: The results of the study have shown that the venom caused changes in the levels of WBC, RBC and Hematocrit. The mean level of WBC at the start was 10234, whereas following the venom injection it reached to the level of 11757( P<0.0007). The mean number for RBC before the treatment was 7509130 and after injection, the number declined to 7065098( P<0.0001). The average amount of Hematocrit before and after injection was 40.087% and 39.0588 %, respectively (P<0.001). Conclusion: Hemiscorpius lepturus venom has some effects on the levels of WBC, RBC and hematocrit of rats. The study of the hematological changes in humans can lead to better study of the effect of this venom and consequently suitable cure for the injured