Rapid detection and purification of sequence specific DNA binding proteins using magnetic separation

In this paper, a method for the rapid identification and purification of sequence specific DNA binding proteins based on magnetic separation is presented. This method was applied to confirm the binding of the human recombinant USF1 protein to its putative binding site (E-box) within the human SOX3 protomer. It has been shown that biotinylated DNA attached to streptavidin magnetic particles specifically binds the USF1 protein in the presence of competitor DNA. It has also been demonstrated that the protein could be successfully eluted from the beads, in high yield and with restored DNA binding activity. The advantage of these procedures is that they could be applied for the identification and purification of any high-affinity sequence-specific DNA binding protein with only minor modifications

Rapid detection and purification of sequence specific DNA binding proteins using magnetic separation

In this paper, a method for the rapid identification and purification of sequence specific DNA binding proteins based on magnetic separation is presented. This method was applied to confirm the binding of the human recombinant USF1 protein to its putative binding site (E-box) within the human SOX3 protomer. It has been shown that biotinylated DNA attached to streptavidin magnetic particles specifically binds the USF1 protein in the presence of competitor DNA. It has also been demonstrated that the protein could be successfully eluted from the beads, in high yield and with restored DNA binding activity. The advantage of these procedures is that they could be applied for the identification and purification of any high-affinity sequence-specific DNA binding protein with only minor modifications

Rethinking conversion : beyond the religious and the secular

This article seeks to pinpoint some of the consequences of the secularization process – conceived as the dissociation of religion from both state politics and culture – with the aim of broadening the conceptualization of conversion. Conversion is therefore considered to be a social fact beyond the religious and the secular, for which the concept of 'trans-formation' is employed in order to grasp this phenomenon. The concept of trans-formation posits processes of conversion as shifts from intimate convictions to public values. From this perspective, based on a pragmatic sociology of values, religious conversions are put forth as one possible way to qualify trajectories that the concept of trans-formation aims to comprehend. The article ends by considering the status of religious convictions in comparison with convictions otherwise qualified