Glutathione as a suitable biomarker in hepatopancreas, gills and muscle of three freshwater crayfish species

We determined the contents of total glutathione (tGSH), reduced glutathione (GSH), and oxidized glutathione (GSSG) and values of the glutathione redox index (GSH RI) in hepatopancreas, gills, and muscle of three freshwater crayfish species: noble crayfish (Astacus astacus) from the Southern Morava River, stone crayfish (Austropotamobius torrentium) from the Krajkovačka River, and spinycheek crayfish (Orconectes limosus) from the Danube River. The obtained data show strong tissue and species specifity of investigated parameters: tGSH, GSH, GSSG, and GSH RI in the hepatopancreas, gills, and muscle of the indicated crayfish species. Our work represents the first study of its kind and showed that the investigated parameters can be considered suitable biomarkers of the cellular glutathione redox status in of freshwater crayfish species.Određivan je sadržaj ukupnog glutationa (tGSH),redukovanog glutationa (GSH),oksidovanog glutationa (GSSG)i glutation redoks indeksa (GSH RI)u hepatopankreasu, škrgama i mišićima tri vrste slatkovodnih rakova: rečnog raka (Astacus astacus) iz reke Južna Morava, raka kamenjara (Austropotamobius torrentium) iz Krajkovačke reke i američkog raka (Orconecteslimosus) iz reke Dunav. Dobijeni podaci pokazuju značajnu tkivnu i specijes specifičnost ispitivanih parametara: GSH, GSSG i GSHRI u hepatopankreasu, škrgama i mišićima kod rakova. Naš rad predstavlja prvu studiju ove vrste i pokazuje da ispitivani parametri mogu biti pogodni biomarkeri ćelijskog redoks statusa glutationa kod slatkovodnih vrsta rakova.Projekat ministarstva br. 143035

Superoxide dismutase and catalase activities in the digestive gland and gills of the freshwater bivalve Unio pictorum from the Sava river

We investigated the potential use of the antioxidant defense enzymes in freshwater mussel (Unio pictorum) as biomarkers of oxidative stress. The enzymatic activities of superoxide dismutase (SOD, EC 1.15.1.1) and catalase (CAT, EC 1.11.1.6), total protein concentration in addition to protein and SOD electrophoretic profiles were examined in the digestive gland and gills of the freshwater bivalve Unio pictorum at two localities on the River Sava. The differences between SOD and CAT activities in examined tissues of freshwater bivalve Unio pictorum reflect dissimilar metabolic and antioxidative activities and this can be the result of both tissue or locality specificities and diverse ecophysiological influences on the organism.Projekat ministarstva br. 173041

Glutathione as a suitable biomarker in hepatopancreas, gills and muscle of three freshwater crayfish species

We determined the contents of total glutathione (tGSH), reduced glutathione (GSH), and oxidized glutathione (GSSG) and values of the glutathione redox index (GSH RI) in hepatopancreas, gills, and muscle of three freshwater crayfish species: noble crayfish (Astacus astacus) from the Southern Morava River, stone crayfish (Austropotamobius torrentium) from the Krajkovačka River, and spinycheek crayfish (Orconectes limosus) from the Danube River. The obtained data show strong tissue and species specifity of investigated parameters: tGSH, GSH, GSSG, and GSH RI in the hepatopancreas, gills, and muscle of the indicated crayfish species. Our work represents the first study of its kind and showed that the investigated parameters can be considered suitable biomarkers of the cellular glutathione redox status in of freshwater crayfish species.Određivan je sadržaj ukupnog glutationa (tGSH),redukovanog glutationa (GSH),oksidovanog glutationa (GSSG)i glutation redoks indeksa (GSH RI)u hepatopankreasu, škrgama i mišićima tri vrste slatkovodnih rakova: rečnog raka (Astacus astacus) iz reke Južna Morava, raka kamenjara (Austropotamobius torrentium) iz Krajkovačke reke i američkog raka (Orconecteslimosus) iz reke Dunav. Dobijeni podaci pokazuju značajnu tkivnu i specijes specifičnost ispitivanih parametara: GSH, GSSG i GSHRI u hepatopankreasu, škrgama i mišićima kod rakova. Naš rad predstavlja prvu studiju ove vrste i pokazuje da ispitivani parametri mogu biti pogodni biomarkeri ćelijskog redoks statusa glutationa kod slatkovodnih vrsta rakova.Projekat ministarstva br. 143035

Superoxide dismutase and catalase activities in the digestive gland and gills of the freshwater bivalve Unio pictorum from the Sava river

We investigated the potential use of the antioxidant defense enzymes in freshwater mussel (Unio pictorum) as biomarkers of oxidative stress. The enzymatic activities of superoxide dismutase (SOD, EC 1.15.1.1) and catalase (CAT, EC 1.11.1.6), total protein concentration in addition to protein and SOD electrophoretic profiles were examined in the digestive gland and gills of the freshwater bivalve Unio pictorum at two localities on the River Sava. The differences between SOD and CAT activities in examined tissues of freshwater bivalve Unio pictorum reflect dissimilar metabolic and antioxidative activities and this can be the result of both tissue or locality specificities and diverse ecophysiological influences on the organism.Projekat ministarstva br. 173041

Prepartal platelet count and fibrinogen concentration in dairy cows with and without retained placenta

Congress Proceeding

The concentrations of antioxidant compounds in the hepatopancreas, the gills and muscle of some freshwater crayfish species

We examined the concentrations of vitamin E ( vit E), sulphydryl groups ( - SH), total protein and protein electrophoretic profiles in the hepatopancreas, the gills and muscle of three freshwater crayfish species: Noble crayfish ( Astacus astacus), stone crayfish ( Austropotamobius torrentium) and spiny cheek crayfish ( Orconectes limosus). Vit E concentration in the hepatopancreas of O. limosus was lower compared to A. astacus, while in the gills of O. limosus it was lower compared to both A. astacus and A. torrentium. The concentration of - SH groups was lower in the hepatopancreas of A. astacus compared to A. torrentium and O. limosus. In the gills of A. astacus and A. torrentium the concentration of - SH groups was higher compared to O. limosus. Protein concentration was higher in the hepatopancreas of A. torrentium compared to A. astacus and O. limosus. A lower protein concentration in muscle of A. torrentium was found compared to O. limosus and A. astacus. Electrophoretic analysis of proteins indicated species and tissue specifities between investigated crayfish species. Our results represent the first study of its kind and provide the basis for future studies that will consider our reported parameters as potential biomarkers for biomonitoring of basic environmental conditions and some anthropogenic impacts.nul

The concentrations of antioxidant compounds in the hepatopancreas, the gills and muscle of some freshwater crayfish species

We examined the concentrations of vitamin E ( vit E), sulphydryl groups ( - SH), total protein and protein electrophoretic profiles in the hepatopancreas, the gills and muscle of three freshwater crayfish species: Noble crayfish ( Astacus astacus), stone crayfish ( Austropotamobius torrentium) and spiny cheek crayfish ( Orconectes limosus). Vit E concentration in the hepatopancreas of O. limosus was lower compared to A. astacus, while in the gills of O. limosus it was lower compared to both A. astacus and A. torrentium. The concentration of - SH groups was lower in the hepatopancreas of A. astacus compared to A. torrentium and O. limosus. In the gills of A. astacus and A. torrentium the concentration of - SH groups was higher compared to O. limosus. Protein concentration was higher in the hepatopancreas of A. torrentium compared to A. astacus and O. limosus. A lower protein concentration in muscle of A. torrentium was found compared to O. limosus and A. astacus. Electrophoretic analysis of proteins indicated species and tissue specifities between investigated crayfish species. Our results represent the first study of its kind and provide the basis for future studies that will consider our reported parameters as potential biomarkers for biomonitoring of basic environmental conditions and some anthropogenic impacts.nul

DOI:10.2298/ABS0703169P GLUTATHIONE DEPENDENT ENZYME ACTIVITIES IN THE FOOT OF THREE FRESHWATER MUSSEL SPECIES IN THE SAVA RIVER, SERBIA

Abstract – We investigated activities of glutathione peroxidase (GSH-Px), glutathione reductase (GR), and the phase II biotransformation enzyme glutathione-S-transferase (GST) in the foot of three freshwater mussel species: Unio pictorum (Up), Unio tumidus (Ut), and Sinanodonta woodiana (Sw) from the Sava River. Specific and total GSH-Px activity was lower in Sw than in Up and Ut. Total GR activity was higher in Up than in Sw. Specific GST activity was higher in Up than in Ut. Total GST activity was higher in Up than in Ut and Sw. Electrophoretic analysis of proteins shows species specifities between the investigated mussel species. Our study represents the first comprehensive report of the investigated glutathione-dependent enzyme activities in the foot of three freshwater mussel species from the Sava River, Serbia

GLUTATHIONE AS А SUITABLE BIOMARKER IN HEPATOPANCREAS, GILLS AND MUSCLE OF THREE FRESHWATER CRAYFISH SPECIES

Abstract — We determined the contents of total glutathione (tGSH), reduced glutathione (GSH), and oxidized glutathione (GSSG) and values of the glutathione redox index (GSH RI) in hepatopancreas, gills, and muscle of three freshwater crayfish species: noble crayfish (Astacus astacus) from the Southern Morava River, stone crayfish (Austropotamobius torrentium) from the Krajkovačka River, and spinycheek crayfish (Orconectes limosus) from the Danube River. The obtained data show strong tissue and species specifity of investigated parameters: tGSH, GSH, GSSG, and GSH RI in the hepatopancreas, gills, and muscle of the indicated crayfish species. Our work represents the first study of its kind and showed that the investigated parameters can be considered suitable biomarkers of the cellular glutathione redox status in of freshwater crayfish species