Rapid Response Command and Control (R2C2): a systems engineering analysis of scaleable communications for Regional Combatant Commanders

Includes supplementary materialDisaster relief operations, such as the 2005 Tsunami and Hurricane Katrina, and wartime operations, such as Operation Enduring Freedom and Operation Iraqi Freedom, have identified the need for a standardized command and control system interoperable among Joint, Coalition, and Interagency entities. The Systems Engineering Analysis Cohort 9 (SEA-9) Rapid Response Command and Control (R2C2) integrated project team completed a systems engineering (SE) process to address the military’s command and control capability gap. During the process, the R2C2 team conducted mission analysis, generated requirements, developed and modeled architectures, and analyzed and compared current operational systems versus the team’s R2C2 system. The R2C2 system provided a reachback capability to the Regional Combatant Commander’s (RCC) headquarters, a local communications network for situational assessments, and Internet access for civilian counterparts participating in Humanitarian Assistance/Disaster Relief operations. Because the team designed the R2C2 system to be modular, analysis concluded that the R2C2 system was the preferred method to provide the RCC with the required flexibility and scalability to deliver a rapidly deployable command and control capability to perform the range of military operations

Crystal structure of AmyB, an alpha-amylase from Halothermothrix orenii, and comparison with its homologs

Ph.DDOCTOR OF PHILOSOPH

A gel elution apparatus with high sample recovery

10.1006/abio.2000.4590Analytical Biochemistry2822258-259ANBC

Bacillus stearothermophilus DNA polymerase I (klenow) clones including those with reduced 3'-to-5' exonuclease activity

EP0875576A2Published Applicatio

Bacillus stearothermophilus DNA polymerase I (klenow) clones including those with reduced 3'-to-5' exonuclease activity

EP0875576A3Published Applicatio

Bacillus stearothermophilus DNA Polymerase I (klenow) clones including those with reduced 3'- to -5' exonuclease activity

US6013451Granted Paten

Expression, purification and preliminary crystallographic analysis of sucrose phosphate synthase (SPS) from Halothermothrix orenii

The first crystallographic study of a sucrose phosphate synthase from H. orenii, an organism that is both thermophilic and halophilic, is reported. The protein crystal diffracts X-rays to 3.01 Å

Shoulder Impingement Syndrome, a Common Affliction of the Shoulder: A Comprehensive Review

The shoulder joint is the most mobile joint in the human body. It comprises a ball and socket structure that enables a series of functional movements to be carried out. These range from simple movements such as reaching for the top shelf to highly explosive overhead activities which involve throwing a baseball or serving with a tennis racquet as seen in athletes. Shoulder impingement syndrome (SIS) is one of the most common shoulder disorders seen in general practice. It is caused by a multitude of factors. Pathology can arise either from the rotator cuff tendon itself, structures external to this tendon or both in combination. The resultant shoulder pain with SIS not only causes distress but also limits the quality of life of patients. This article aims to provide a comprehensive review of the pathogenesis, aetiologies, clinical tests, investigations and management options for SIS based on current literature and research

A novel cytosolic NADH:quinone oxidoreductase from Methanothermobacter marburgensis

Methanothermobacter marburgensis is a strictly anaerobic, thermophilic methanogenic archaeon that uses methanogenesis to convert H2 and CO2 to energy. M. marburgensis is one of the best-studied methanogens, and all genes required for methanogenic metabolism have been identified. Nonetheless, the present study describes a gene (Gene ID 9704440) coding for a putative NAD(P)H:quinone oxidoreductase that has not yet been identified as part of the metabolic machinery. The gene product, MmNQO, was successfully expressed, purified and characterized biochemically, as well as structurally. MmNQO was identified as a flavin-dependent NADH:quinone oxidoreductase with the capacity to oxidize NADH in the presence of a wide range of electron acceptors, whereas NADPH was oxidized with only three acceptors. The 1.50 Å crystal structure of MmNQO features a homodimeric enzyme where each monomer comprises 196 residues folding into flavodoxin-like α/β domains with non-covalently bound FMN (flavin mononucleotide). The closest structural homologue is the modulator of drug activity B from Streptococcus mutans with 1.6 Å root-mean-square deviation on 161 Cα atoms and 28% amino-acid sequence identity. The low similarity at sequence and structural level suggests that MmNQO is unique among NADH:quinone oxidoreductases characterized to date. Based on preliminary bioreactor experiments, MmNQO could provide a useful tool to prevent overflow metabolism in applications that require cells with high energy demand