Complete mitochondrial genome of Corydoras duplicareus (Teleostei, Siluriformes, Callichthyidae)

In this work, we reported the complete mitochondrial genome of Corydoras duplicareus. The total length of the mitochondrial genome is 16,667 bp, with the base composition of 32.90% A, 26.55% T, 25.94% C, and 14.61% G. It contains two ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes, and a major non-coding control region (D-loop region). The arrangement of these genes is the same as that found in the Siluriformes. All the protein initiation codons are ATG, except for cox1 that begins with GTG. The cytochrome c oxidase subunit I (COX1) amino acid sequence of C. duplicareus and other 27 species from 8 genera were used for phylogenetic analysis by maximum likelihood method. The topology demonstrated that all species are divided into two groups (Callichthyidae and Cyprinidae), and the C. duplicareus was clustered with C.nattereri

Ci-AMBP: a highly conserved member of the microglobulin superfamily of proteinase inhibitors in grass carp, Ctenopharyngodon idellus

A full-length cDNA clone encoding grass carp (Ctenopharyngodon idellus) α1-microglobulin/bikunin precursor (Ci-AMBP) was isolated by subtracted differential hybridization screening from a liver cDNA library. The deduced amino acid sequence shared approximately 50% sequence identity with its mammalian counterparts, but more than 90% identity with another fish species. AMBPs are the precursors of the plasma glycoproteins α1-microglobulin (α1m) and bikunin. Both peptide structures and their chromosomal organization were well conserved in Ci-AMBP. The α1m and bikunin polypeptides are separated by the typical tetrapeptide R-A-R-R that provides an endoproteolytic cleavage site for maturation. The genetic organization of domains and functional motifs indicated that Ci-AMBP is a typical member of the lipocalin and Kunitz-type protease inhibitor superfamilies. Expression of the Ci-AMBP gene in different tissues/organs was evaluated using semi-quantitative RT-PCR and, in contrast to the restricted expression in other species, transcripts were detected in a wide range of tissues. The most abundant expression occurred in the secretory organs, which supports the roles of α1m and bikunin in the immune response to diseases and in the stress response.This work was supported by the Young Scientist Research Foundation of Hunan Educational Committee (Grant No. 10B046) and by the Hunan Committee of Science and Technology, China (Grant No. 2012NK3078)

Next-generation sequencing yields the complete mitochondrial genome of Corydoras sterbai (Teleostei, Siluriformes, Callichthyidae, Corydoradinae)

In this work, we reported the complete mitochondrial genome yielded from next-generation sequencing of Corydoras sterbai. The total length of the mitochondrial genome is 16,636 bp, with the base composition of 32.70% A, 26.40% T, 26.00% C, and 14.90% G. It contains two ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes, and a major non-coding control region (D-loop region). The arrangement of these genes is the same as that found in the Siluriformes. All the protein initiation codons are ATG, except for cox1 that begins with GTG. The Neighbour-Joining tree built using Geneious showed that C. sterbai was clustered with other species from the genus Corydoras. It means that its external morphological feature classification is consistent with the molecular classification results

Characterization and phylogenetic analysis of Corydoras arcuatus mitochondrial genome

Corydoras arcuatus is a kind of famous and precious ornamental fish. In this study, the complete mitochondrial genome yielded by next-generation sequencing of Corydoras arcuatus was assembled and analyzed. The total length of the mitochondrial genome is 16,822 bp, with the base composition of 32.40% A, 26.10% T, 26.30% C, and 15.20% G. It contains two ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes, and a major non-coding control region (D-loop region). The arrangement of these genes is the same as that found in the Siluriformes. All the protein initiation codons are ATG, except for cox1 that begins with GTG. The complete mitogenomes of C. arcuatus and other 17 species from nine genera were used for phylogenetic analysis by Neighbour-Joining method. The topology demonstrated that all species are divided into three groups (Siluridae, Loricariidae, and Callichthyidae), and the C. arcuatus was clustered with C. rabauti and C. nattereri

Characterization and phylogenetic analysis of Corydoras trilineatus mitochondrial genome

In this study, the complete mitochondrial genome yielded by next-generation sequencing of C. trilineatus was assembled and analyzed. The total length of the mitochondrial genome is 16,526 bp. It contains two ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes, and a major non-coding control region . The arrangement of these genes is the same as that found in the Siluriformes. The complete mitogenomes of C. trilineatus and other 17 species from nine genera were used for phylogenetic analysis by UPGMA method. The topology demonstrated that all species are divided into three groups , and the C. trilineatus was clustered with C. rabauti and C. nattereri

Sexual Dimorphism and Discrimination of Barbel Steed (<i>Hemibarbus labeo</i>) in the Jinhe River, China: An Indicator of Habitat Status

Sex identification is linked to sexual dimorphism and is an important study issue in fish biology and aquaculture. However, owing to the unmarked sexual heteromorphism between adult Hemibarbus labeo, it is often difficult to distinguish their sex by visual observation. This study aimed to find a simple and reliable morphometric criterion for the sex identification of H. labeo using discriminant models. Forty-two morphometric traits of sixty-eight H. labeo individuals collected from the Jinhe River were measured, and 41 standardized features were calculated and analyzed. Eight trait variables from 41 standardized attributes were screened using stepwise discriminant analysis. The total classification accuracy of the model was 95.59%. Twelve standardized features significantly differed between male and female H. labeo individuals (p p H. labeo in the Jinhe River were plumper than males, with a larger body size, but a smaller caudal peduncle. These results implied that the sex identification of H. labeo can be performed using the discriminant equation established in this study. This study provides a theoretical basis for endangered fish species protection and their artificial propagation

Complete mitochondrail genome of Corydoras panda (Teleostei, Siluriformes, Callichthyidae, Corydoradinae)

We reported the complete mitochondrial genome yielded by next-generation sequencing of Corydoras panda in this study. The total length of the mitochondrial genome is 16,611 bp, with the base composition of 32.50% A, 26.30% T, 26.10% C, and 15.10% G, respectively. It contains two ribosomal RNA genes, 13 protein-coding genes, 22 transfer RNA genes, and a major non-coding control region (D-loop region). The arrangement of these genes is the same as that found in the Siluriformes. The complete mitogenomes of C. panda and other 18 species from Siluriformes were used for phylogenetic analysis using Neighbor-Joining method. The topology demonstrated that all species belong to nine genera are divided into three groups (Siluridae, Loricariidae, and Callichthyidae), and the C. panda was clustered with other species from genus Corydoras. Corydoras panda external morphological feature classification is consistent with the molecular classification results, so the information of the mitogenome could be used for future identification of Corydoras species

Complete mitochondrail genome of Corydoras agassizii

We reported the complete mitochondrial genome yielded using next-generation sequencing of Corydoras agassizii in this study. The total length of the mitochondrial genome is 16,562 bp, with the base composition of 32.6% A, 25.9% T, 26.8% C, and 14.7% G, in several. It contains two rRNA genes, 22 tRNA genes, 13 protein-coding genes, and a 945 bp non-coding control region (D-loop region). The sequence of these genes is consistent with that found in the Siluriformes. The complete mitogenomes of C. agassizii and other 17 species of fish were constructed by phylogenetic analysis using Neighbour-Joining method. The topological structure indicated that species participating in the analysis belong to three groups (Siluridae, Loricariidae, and Callichthyidae) of nine genera, and the C. agassizii was clustered with other species from genus Corydoras. The external morphological characteristics of C. agassizii are consistent with the results of molecular classification, so the mitogenome can be used to identify Corydoras species in the future