Study on Inlet Flow Field Structure and End-Wall Effect of Axial Flow Pump Impeller under Design Condition

The existing experimental technology cannot accurately and quantitatively measure the flow field structure and the wall boundary layer displacement effect in the axial flow pump. Based on SST k-ω turbulence model, a three-dimensional unsteady numerical simulation of the whole flow field of an axial flow pump was presented at the designed operating point to overcome the weakness of traditional measurement methods in measuring the flow field of the axial flow pump. The flow field structure of the axial flow pump inlet was studied quantitatively and the result was compared with the theoretical design value. It was found that there is an obvious impeller rotation effect and end-wall effect in the flow field of the axial flow pump inlet. The distribution law of the impeller inlet flow field and the crowding coefficient caused by the wall boundary layer were obtained. The pump inlet measurement point in the experiment and calculation domain inlet in the simulation should be kept at a distance of more than 0.5 Ds away from the impeller inlet to eliminate the influence of the impeller rotation effect. Through contrastive analysis, it was found that there is an obvious difference between the calculated value and the design value of the flow field structure due to the end-wall effect. The crowding coefficient should be taken into account when designing an axial flow pump. This study has certain reference significance for further understanding the flow field structure at the inlet of the axial flow pump impeller and improving the design theory of the axial flow pump

Study on Inlet Flow Field Structure and End-Wall Effect of Axial Flow Pump Impeller under Design Condition

The existing experimental technology cannot accurately and quantitatively measure the flow field structure and the wall boundary layer displacement effect in the axial flow pump. Based on SST k-ω turbulence model, a three-dimensional unsteady numerical simulation of the whole flow field of an axial flow pump was presented at the designed operating point to overcome the weakness of traditional measurement methods in measuring the flow field of the axial flow pump. The flow field structure of the axial flow pump inlet was studied quantitatively and the result was compared with the theoretical design value. It was found that there is an obvious impeller rotation effect and end-wall effect in the flow field of the axial flow pump inlet. The distribution law of the impeller inlet flow field and the crowding coefficient caused by the wall boundary layer were obtained. The pump inlet measurement point in the experiment and calculation domain inlet in the simulation should be kept at a distance of more than 0.5 Ds away from the impeller inlet to eliminate the influence of the impeller rotation effect. Through contrastive analysis, it was found that there is an obvious difference between the calculated value and the design value of the flow field structure due to the end-wall effect. The crowding coefficient should be taken into account when designing an axial flow pump. This study has certain reference significance for further understanding the flow field structure at the inlet of the axial flow pump impeller and improving the design theory of the axial flow pump

Formation and Microbial Composition of Biofilms in Drip Irrigation System under Three Reclaimed Water Conditions

As the second source of water for cities, reclaimed water (RW) has become an effective solution to the problem of water scarcity in modern agriculture. However, the formation of biofilm in an RW distribution system seriously affects the performance of the system and has become a technical challenge in RW utilization. In this study, we first showed that several water quality parameters, including five-day biochemical oxygen demand (BOD5), total bacteria count (TB), total nitrogen (TN), and Cl− were the main factors affecting biofilm accumulation in the drip irrigation system (DIS), with the correlation coefficient averaging above 0.85. Second, after 392 to 490 h of system operation, the total biomass and extracellular polymer (EPS) accumulation rate of biofilms increased to a maximum of 0.72 g/m2·h and 0.027g/m2·h, respectively, making this time point a critical point for controlling biofilm accumulation and clogging of the system. Third, we examined changes in biofilm microbial composition over time on Illumina’s MiSeq platform. High throughput sequencing data showed that bacterial community structure and microbial network interaction and modularity changed significantly between 392 and 490 h, resulting in maximum microbial diversity and community richness at 490 h. Spearman correlation analyses between genera revealed that Sphingomonas and Rhodococcus promote biofilm formation due to their hydrophobicity, while Bacillus, Mariniradius, and Arthronema may inhibit biofilm formation due to their antagonistic effects on other genera. In conclusion, this work has clarified the accumulation process and compositional changes of biofilms in agriculture DIS under different RW conditions, which provides a basis for improving RW utilization efficiency and reducing system maintenance costs

Formation and Microbial Composition of Biofilms in Drip Irrigation System under Three Reclaimed Water Conditions

As the second source of water for cities, reclaimed water (RW) has become an effective solution to the problem of water scarcity in modern agriculture. However, the formation of biofilm in an RW distribution system seriously affects the performance of the system and has become a technical challenge in RW utilization. In this study, we first showed that several water quality parameters, including five-day biochemical oxygen demand (BOD5), total bacteria count (TB), total nitrogen (TN), and Cl− were the main factors affecting biofilm accumulation in the drip irrigation system (DIS), with the correlation coefficient averaging above 0.85. Second, after 392 to 490 h of system operation, the total biomass and extracellular polymer (EPS) accumulation rate of biofilms increased to a maximum of 0.72 g/m2·h and 0.027g/m2·h, respectively, making this time point a critical point for controlling biofilm accumulation and clogging of the system. Third, we examined changes in biofilm microbial composition over time on Illumina’s MiSeq platform. High throughput sequencing data showed that bacterial community structure and microbial network interaction and modularity changed significantly between 392 and 490 h, resulting in maximum microbial diversity and community richness at 490 h. Spearman correlation analyses between genera revealed that Sphingomonas and Rhodococcus promote biofilm formation due to their hydrophobicity, while Bacillus, Mariniradius, and Arthronema may inhibit biofilm formation due to their antagonistic effects on other genera. In conclusion, this work has clarified the accumulation process and compositional changes of biofilms in agriculture DIS under different RW conditions, which provides a basis for improving RW utilization efficiency and reducing system maintenance costs

Long-Distance Crosswell EM Logging of Copper Ore Using Borehole-Surface Current Injection in Slim Holes

Crosswell electromagnetic (EM) methods are widely used in subsurface geophysical prospecting because they can achieve more effective long-distance detection than single-well methods. However, a large-diameter borehole is required to increase the magnetic moment of the magnetic dipole source. For the long-distance detection of copper ores, which is usually performed in slim holes, we present a borehole-surface current-injection-based crosswell EM logging method. Considering the cost of deploying casing, we inject a low-frequency ac directly into the ground, and converging current is formed around low-resistance anomalies in the formation. Then, the distribution of the anomalies can be inferred by detecting the low-frequency alternating magnetic field of the converging current in the receiver well. Moreover, to further improve the detection performance, we design a placement scheme for the grounding electrode for multianomaly crosswell detection based on the Gauss–Newton inversion algorithm, where the EM responses for different grounding electrode locations are analyzed. Field experiments are conducted using two slim open holes spaced approximately 1000 m apart for the detection of two copper ores. Through the processing and interpretation of measured EM signals, the conductivity imaging results of the crosswell EM method indicate that the measured distribution of anomalies is consistent with prior knowledge obtained from numerous single-well loggings, demonstrating the feasibility of the proposed application for long-distance crosswell EM logging in slim open holes

FNDC5 Promotes Adipogenic Differentiation of Primary Preadipocytes in Mashen Pigs

Fibronectin type III domain-containing protein 5 (FNDC5) plays an important role in fat deposition, which can be cut to form Irisin to promote fat thermogenesis, resulting in a decrease in fat content. However, the mechanism of FNDC5 related to fat deposition in pigs is still unclear. In this research, we studied the expression of FNDC5 on different adiposes and its function in the adipogenic differentiation of primary preadipocytes in Mashen pigs. The expression pattern of FNDC5 was detected by qRT-PCR and Western blotting in Mashen pigs. FNDC5 overexpression and interference vectors were constructed and transfected into porcine primary preadipocytes by lentivirus. Then, the expression of key adipogenic genes was detected by qRT-PCR and the content of lipid droplets was detected by Oil Red O staining. The results showed that the expression of FNDC5 in abdominal fat was higher than that in back subcutaneous fat in Mashen pigs, whereas the expression in back subcutaneous fat of Mashen pigs was significantly higher than that of Large White pigs. In vitro, FNDC5 promoted the adipogenic differentiation of primary preadipocytes of Mashen pigs and upregulated the expression of genes related to adipogenesis, but did not activate the extracellular signal-regulated kinase (ERK) signaling pathway. This study can provide a theoretical basis for FNDC5 in adipogenic differentiation in pigs