6 research outputs found
(+)-N-(2-Hydroxypropyl)lindcarpine: A New Cytotoxic Aporphine Isolated from Actinodaphne pruinosa Nees
No full textOnenewalkaloid; (+)-N-(2-hydroxypropyl)lindcarpine (1),together with four known aporphine alkaloids, (+)-boldine (2) (+)-norboldine (3), (+)-lindcarpine (4) and (+)-methyllindcarpine (5) were isolated from the stem bark of Actinodaphne pruinosa Nees (Lauraceae). (+)-N-(2-Hydroxypropyl)lindcarpine (1) exhibited cytotoxic activity against P-388 murine leukemia cells with an IC50 value of 3.9 μg/mL. Structural elucidation of all the compounds were performed by spectral methods such as 1D- and 2D- NMR, IR, UV, and HRESIMS
LC-HRMS-based metabolomics to evaluate the phytochemical profile and antioxidant capacity of Cosmos caudatus with different extraction methods and solvents
No full textCosmos caudatus, a medicinal plant used in traditional medicine, is also consumed as a vegetable. This study aimed to determine the total phenolics, total flavonoids, antioxidant activity, and metabolite profiles of C. caudatus extracts obtained using different extraction methods (direct and successive macerations) and solvents. In addition, metabolite profiles were analyzed using principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) to distinguish samples and correlate them with their antioxidant activity. The total phenolic and flavonoid content in C. caudatus varied from 97.03 to 472.60 mg gallic acid equivalent/g dried sample and 24.56–68.00 mg quercetin equivalent/g dried sample. Ethanol extracts obtained through direct maceration method showed the highest antioxidant capacity. PCA using LC-HRMS chromatogram data distinguished the samples with a total PC of 95.1% (positive ionization mode) and 98.5% (negative ionization mode). Using PLS-DA, we identified several compounds, such as isoquercitrin, quercetin-3-O-rutinoside, avicularin, rutin, quercitrin, and vitexin, which likely contribute to the antioxidant capacity of C. caudatus
